Filter-Paper Electrophoresis of Tears

1956 ◽  
Vol 55 (6) ◽  
pp. 800 ◽  
Author(s):  
O. FEDDE ERICKSON
1956 ◽  
Vol 2 (3) ◽  
pp. 145-159 ◽  
Author(s):  
Joseph T Anderson ◽  
Ancel Keys

Abstract 1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.


Science ◽  
1952 ◽  
Vol 115 (2997) ◽  
pp. 626-627 ◽  
Author(s):  
F. Larson ◽  
W. P. Deiss ◽  
E. C. Albright

1954 ◽  
Vol 3 (4) ◽  
pp. 263-269 ◽  
Author(s):  
IAN R. MACKAY ◽  
WADE VOLWILER

1954 ◽  
Vol 32 (1) ◽  
pp. 567-570
Author(s):  
S. D. Vesselinovitch ◽  
H. S. Funnell

A technique for filter paper electrophoresis and strip staining that permits the running time to be reduced to two hours or less is described. Some typical two hour electrophoretic patterns are illustrated and the clinical advantages of the technique mentioned. Details of a simple and inexpensive apparatus for electrophoresis on filter paper are given. Several variables affecting rapid and distinct protein separation, which were modified in the development of this method, and the relationships between these changes and the shortened running time are discussed.


1966 ◽  
Vol 46 (4_ts) ◽  
pp. 483-485 ◽  
Author(s):  
Melvin B. Black ◽  
Hugh Miller ◽  
Jacqueline Wan

1981 ◽  
Vol 34 (2) ◽  
pp. 149 ◽  
Author(s):  
K Bell ◽  
KE Hopper ◽  
HA McKenzie

An electrophoretic examination is made of milk samples taken from eight Bali (banteng) cattle, Bos (Bibos) javanicus, at Beatrice Hills, Northern Territory, Australia. Starch-gel electrophoresis at pH 8� 5 (NaOH-H3B03 buffer) and filter-paper electrophoresis at pH 8� 6 (diethylbarbiturate buffer) indicate that all samples contain a new a-lactalbumin variant, designated a-lactalbumin C. The order of mobility for bovine variants is A > B > C. The C variant differs from the common B variant in having one more amide residue (substitution of Asn for Asp or GIn for Glu).


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