Localization of Protein-bound Radioactive Iodine by Filter Paper Electrophoresis

Science ◽  
1952 ◽  
Vol 115 (2997) ◽  
pp. 626-627 ◽  
Author(s):  
F. Larson ◽  
W. P. Deiss ◽  
E. C. Albright
Keyword(s):  
Filter Paper ◽  
Radioactive Iodine ◽  
Paper Electrophoresis

Cholesterol in Serum and Lipoprotein Fractions

1956 ◽  
Vol 2 (3) ◽  
pp. 145-159 ◽  
Author(s):  
Joseph T Anderson ◽  
Ancel Keys
Keyword(s):  
Human Serum ◽  
Total Cholesterol ◽  
Filter Paper ◽  
Room Temperature ◽  
Paper Electrophoresis ◽  
Cholesterol Content ◽  
Intraindividual Variability ◽  
Detailed Data ◽  
The Stability ◽  
Source Of Error

Abstract 1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.

QUANTITATIVE ASPECTS OF FILTER PAPER ELECTROPHORESIS OF SERUM

1954 ◽  
Vol 3 (4) ◽  
pp. 263-269 ◽  
Author(s):  
IAN R. MACKAY ◽  
WADE VOLWILER
Keyword(s):  
Filter Paper ◽  
Paper Electrophoresis

A RAPID METHOD FOR FILTER PAPER ELECTROPHORESIS

1954 ◽  
Vol 32 (1) ◽  
pp. 567-570
Author(s):  
S. D. Vesselinovitch ◽  
H. S. Funnell
Keyword(s):  
Rapid Method ◽  
Filter Paper ◽  
Protein Separation ◽  
Paper Electrophoresis ◽  
Running Time ◽  
Electrophoretic Patterns ◽  
Several Variables

A technique for filter paper electrophoresis and strip staining that permits the running time to be reduced to two hours or less is described. Some typical two hour electrophoretic patterns are illustrated and the clinical advantages of the technique mentioned. Details of a simple and inexpensive apparatus for electrophoresis on filter paper are given. Several variables affecting rapid and distinct protein separation, which were modified in the development of this method, and the relationships between these changes and the shortened running time are discussed.

BIOCOLLOIDS IN NORMAL HUMAN URINE: I. AMOUNT AND ELECTROPHORETIC CHARACTERISTICS

1958 ◽  
Vol 36 (1) ◽  
pp. 1159-1166 ◽  
Author(s):  
T. Webb ◽  
B. Rose ◽  
A. H. Sehon
Keyword(s):  
Human Serum ◽  
Electrophoretic Mobility ◽  
Human Urine ◽  
Filter Paper ◽  
Radioactive Iodine ◽  
Normal Serum ◽  
Γ Globulins ◽  
Normal Human ◽  
Normal Urine ◽  
Serum Components

The biocolloids of normal urine have been isolated and characterized by free electrophoresis and electrophoresis on filter paper. An average of 133 mg of material was recovered from 24-hour aliquots of normal urine. This material was composed of at least seven components as revealed by free electrophoresis at pH 8.6. Five of these components were similar in electrophoretic mobility to the five serum components. A relatively large amount of material was present which behaved like the acid mucoproteins of normal serum. No lipoproteins were detected. Some of the components of the urinary biocolloids were shown to be derived from human serum γ-globulins by labelling the latter with radioactive iodine.

Quantitative Determination of A2 Hemoglobin by Filter Paper Electrophoresis

1966 ◽  
Vol 46 (4_ts) ◽  
pp. 483-485 ◽  
Author(s):  
Melvin B. Black ◽  
Hugh Miller ◽  
Jacqueline Wan
Keyword(s):  
Quantitative Determination ◽  
Filter Paper ◽  
Paper Electrophoresis
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