Secretory Cells in the Middle Ear Mucosa of the Guinea Pig: Cytochemical and Ultrastructural Study

1969 ◽  
Vol 89 (5) ◽  
pp. 691-699 ◽  
Author(s):  
B. Hussl ◽  
D. J. Lim
Keyword(s):  
Guinea Pig ◽  
Middle Ear ◽  
Ultrastructural Study ◽  
Secretory Cells ◽  
Middle Ear Mucosa

Regenerated Middle Ear Mucosa after Tympanoplasty. Part I. Transmission Electron Microscopy

1986 ◽  
Vol 94 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Roberto Gamoletti ◽  
Paola Poggi ◽  
Mario Sanna ◽  
Carlo Zini
Keyword(s):  
Electron Microscopy ◽  
Middle Ear ◽  
Goblet Cells ◽  
Secretory Cells ◽  
Canal Wall ◽  
Ciliated Cells ◽  
Middle Ear Mucosa ◽  
Morphologic Characteristics ◽  
Transmission Electron ◽  
Ultrastructural Appearance

The ultrastructural appearance of the regenerated middle ear mucosa—found at the second operation of staged intact canal wall tympanoplasty (ICWT) with mastoidectomy—has been evaluated with the transmission electron microscope. The regenerated epithelium showed all the morphologic characteristics of the normal middle ear mucosa: ciliated cells, noncillated cells, and secretory cells. All of these (Including goblet cells) have been found in the specimens. It is concluded that a normal middle ear mucosa regenerates to cover all denuded bone surfaces after the first operation of staged ICWT with mastoidectomy, when silicone rubber sheeting has been used to prevent adhesions and maintain an air-containing middle ear space.

Ultrastructure of the Middle Ear Mucosa

1976 ◽  
Vol 85 (2_suppl) ◽  
pp. 30-35 ◽  
Author(s):  
Erwin Hentzer
Keyword(s):  
Middle Ear ◽  
Secretory Granules ◽  
Goblet Cells ◽  
Respiratory Epithelium ◽  
Secretory Cells ◽  
Secretory Product ◽  
Ciliated Cells ◽  
Loose Connective Tissue ◽  
Middle Ear Mucosa ◽  
Subepithelial Layer

Histologic studies of recent years have definitely concluded that the epithelium of the middle ear is a modified respiratory epithelium with ciliated and secretory cells, including goblet cells. These cells, like the ciliated cells, are developd from the basal cell and are just ordinary secretory cells completely filled with secretory granules. For different reasons it is postulated, that the dark secretory granules are prestages of the actual secretory product presenting as light granules and that dark and light granules do not represent different secretory capacities of the cell. The subepithelial layer which consists of loose connective tissue is a structure of just as great importance as the epithelial layer.

The capillaries of the middle ear mucosa in the guinea pig

1973 ◽  
Vol 143 (4) ◽  
pp. 517-526 ◽  
Author(s):  
Rolf Haye ◽  
Torgeir Vegge
Keyword(s):  
Guinea Pig ◽  
Middle Ear ◽  
Middle Ear Mucosa

Fine Structures of the Normal Mucosa in Developing Rat Middle Ear

1976 ◽  
Vol 85 (5_suppl2) ◽  
pp. 1-19 ◽  
Author(s):  
Shoichi Maeda ◽  
Goro Mogi ◽  
Motow Oh
Keyword(s):  
Middle Ear ◽  
Secretory Granules ◽  
Transitional Zone ◽  
Normal Mucosa ◽  
Secretory Cells ◽  
Ciliated Cells ◽  
Middle Ear Mucosa ◽  
Fine Structures ◽  
Developing Rat ◽  
Fetal Stage

Forty-two fetal and 90 developing young rats were studied electron microscopically to obtain a normal cellular distribution pattern of middle ear mucosa. The results showed that while ciliated columnar or cuboidal cells interspersed with secretory cells were predominantly distributed around the tympanic orifice and transitional zone, cuboidal or squamous nonciliated cells without secretory granules were numerous in the remaining part of the bulla. Development of ciliated cells started at the 17th or 18th fetal day, paralleling that of secretory cells. The number of ciliated and secretory cells increased rapidly after birth. Glands were not found in the osseous tube, tympanic orifice, or other portions of the middle ear cavity in fetal, newborn, or suckling rats, while they are abundant in the cartilaginous portion of the tube. The findings of this study indicate that both secretory and ciliated cells are normal components of middle ear mucosa although these cells are restricted to the tympanic orifice and transitional zone, and that the mucociliary defense system starts to develop during the fetal stage.

Secretory Lysozyme of the Human Middle Ear Mucosa: Immunocytochemical Localization

1976 ◽  
Vol 85 (1) ◽  
pp. 50-60 ◽  
Author(s):  
David J. Lim ◽  
Yea S. Liu ◽  
Herbert Birck
Keyword(s):  
Middle Ear ◽  
Secretory Cells ◽  
Immunocytochemical Localization ◽  
Biochemical Finding ◽  
Middle Ear Mucosa ◽  
Specific Granules ◽  
Neutrophilic Leukocytes ◽  
Morphological Finding ◽  
Human Middle Ear ◽  
Serous Otitis Media

Lysozyme was demonstrated by an immunocytochemical technique in the biopsied mucosa obtained from the promontory of the fifteen patients who had chronic middle ear effusions. Lysozyme was localized in the mucigen granules of the secretory cells, as well as in the specific granules of the polymorphonuclear neutrophilic leukocytes (PMN) and macrophages. The specimens obtained from patients with mucous effusions showed numerous secretory cells that contained lysozyme, in sharp contrast to the serous type in which only a few secretory cells could be found. The present morphological finding was in agreement with the biochemical finding which demonstrated higher lysozyme level in mucous effusions than that of the serous type. It was concluded that human middle ear mucosa provided lysozyme and that its secretion was active in serous otitis media, particularly of mucoid type.

Functional Morphology of the Lining Membrane of the Middle Ear and Eustachian Tube

1974 ◽  
Vol 83 (11_suppl) ◽  
pp. 5-26 ◽  
Author(s):  
David J. Lim
Keyword(s):  
Acid Phosphatase ◽  
Epithelial Cells ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Close Association ◽  
Active Agent ◽  
Dynamic State ◽  
Secretory Cells ◽  
Defense System ◽  
Middle Ear Mucosa

A large part of normal middle ear cavity and Eustachian tube is provided with a mucociliary transportation system which is considered the first line of defense of the middle ear. Secretory cells of the normal mucosa of the middle ear and the tube can be classified into three main types: mucous, mixed and dark granulated (or serous). This may mean that the secreta of the mucosa is biochemically diverse and/or in a dynamic state. The presence of immunoglobulin-producing plasma cells (IgA, IgG and IgM) and macrophages in the Eustachian tube and the middle ear mucosa supports the concept that the middle ear is protected by an immunologic defense system. Normal mucosal epithelial cells of the middle ear and the tube possess the ability to transport macromolecules by intact epithelial cells toward connective tissue layer where the macromolecules are either phagocytized by histiocytes or enter into capillary system. The macrophages of regional lymph nodes also picked up the macromolecules. Demonstration of lamellar substances similar to phospholipids in the secreta of the tube and middle ear mucosa of the guinea pig suggests the presence of auditory surface-active agent(s) (surfactant). Close association between secretory cells of the mucosa and acid phosphatase is demonstrated by cytochemical method. It is suggested that secretory enzymes such as lysozyme and acid phosphatase may be either produced or transported by secretory cells of the middle ear mucosa. These enzyme secretions are considered a part of the enzymatic defense system of the middle ear.

Atypical Cilia in Normal Human and Guinea Pig Middle Ear Mucosa

1969 ◽  
Vol 67 (2-6) ◽  
pp. 511-515 ◽  
Author(s):  
I. Kawabata ◽  
M. Paparella
Keyword(s):  
Guinea Pig ◽  
Middle Ear ◽  
Middle Ear Mucosa ◽  
Normal Human
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