Secretory Lysozyme of the Human Middle Ear Mucosa: Immunocytochemical Localization

1976 ◽  
Vol 85 (1) ◽  
pp. 50-60 ◽  
Author(s):  
David J. Lim ◽  
Yea S. Liu ◽  
Herbert Birck
Keyword(s):  
Middle Ear ◽  
Secretory Cells ◽  
Immunocytochemical Localization ◽  
Biochemical Finding ◽  
Middle Ear Mucosa ◽  
Specific Granules ◽  
Neutrophilic Leukocytes ◽  
Morphological Finding ◽  
Human Middle Ear ◽  
Serous Otitis Media

Lysozyme was demonstrated by an immunocytochemical technique in the biopsied mucosa obtained from the promontory of the fifteen patients who had chronic middle ear effusions. Lysozyme was localized in the mucigen granules of the secretory cells, as well as in the specific granules of the polymorphonuclear neutrophilic leukocytes (PMN) and macrophages. The specimens obtained from patients with mucous effusions showed numerous secretory cells that contained lysozyme, in sharp contrast to the serous type in which only a few secretory cells could be found. The present morphological finding was in agreement with the biochemical finding which demonstrated higher lysozyme level in mucous effusions than that of the serous type. It was concluded that human middle ear mucosa provided lysozyme and that its secretion was active in serous otitis media, particularly of mucoid type.

Are mucin genes yet to be cloned expressed by human middle ear mucosa?

1998 ◽  
Vol 23 (3) ◽  
pp. 265-265 ◽  
Author(s):  
Hutton ◽  
Birchall ◽  
French ◽  
Kubba ◽  
Severn ◽  
...  
Keyword(s):  
Middle Ear ◽  
Middle Ear Mucosa ◽  
Mucin Genes ◽  
Human Middle Ear

scholarly journals Immunohistochemical and qPCR Detection of SARS-CoV-2 in the Human Middle Ear Versus the Nasal Cavity: Case Series

2021 ◽  
Author(s):  
Arwa Kurabi ◽  
Kwang Pak ◽  
Adam S. DeConde ◽  
Allen F. Ryan ◽  
Carol H. Yan
Keyword(s):  
Nasal Cavity ◽  
Middle Ear ◽  
Viral Infections ◽  
Case Series ◽  
Middle Ear Mucosa ◽  
Viral Receptor ◽  
Tissue Samples ◽  
Angiotensin Converting Enzyme 2 ◽  
Nasal Tissue ◽  
Human Middle Ear

AbstractViral infections have already been implicated with otitis media and sudden sensorineural hearing loss. However, the pathophysiology of COVID-19 as it relates to otologic disorders is not well-defined. With the spread of SARS-CoV-2, it is important to evaluate its colonization of middle ear mucosa. Middle ear and nasal tissue samples for quantitative RT-PCR and histologic evaluations were obtained from post-mortem COVID-19 patients and non-diseased control patients. Here we present evidence that SARS-CoV-2 colonizes the middle ear epithelium and co-localizes with the primary viral receptor, angiotensin-converting enzyme 2 (ACE2). Both middle ear and nasal epithelial cells show relatively high expression of ACE2, required for SARS-CoV-2 entry. The epithelial cell adhesion molecule (EpCAM) was use as a biomarker of epithelia. Furthermore, we found that the viral load in the middle ear is lower than that present in the nasal cavity.

Serous Otitis Media

1973 ◽  
Vol 82 (3) ◽  
pp. 297-301 ◽  
Author(s):  
Robert W. Veltri ◽  
Philip M. Sprinkle
Keyword(s):  
Otitis Media ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Secretory Iga ◽  
Secretory Cells ◽  
Sterile Condition ◽  
Local Synthesis ◽  
Serous Otitis Media ◽  
Inflammatory Insult ◽  
Partial Vacuum

The middle ear fluids of seven patients with bilateral, and five patients with unilateral serous otitis media (SOM), were demonstrated to be microbiologically sterile when assessed for the presence of bacteria, mycoplasma, viruses, and fungi. The concentrations of immunoglobulins G, M, A, D, and lysozyme (muramidase) were determined in the serum and middle ear fluids. Lysozyme levels of middle ear aspirates were found to be elevated in SOM patients. The elevated levels of lysozyme in combination with the antibody-containing classes of immunoglobulins may explain the microbiologically sterile condition of the middle ear fluids of SOM patients. Also, the elevated lysozyme concentrations in middle ear fluids may indicate the previous presence of neutrophils and hence a previous inflammatory insult. The increased levels of IgA demonstrated in middle ear fluids may indicate local synthesis of secretory IgA by secretory cells of Eustachian tube and middle ear. The closed Eustachian tube, partial vacuum conditions and absence of a portal of exit for accumulated serous fluids are offered as a possible explanation for SOM.

Regenerated Middle Ear Mucosa after Tympanoplasty. Part I. Transmission Electron Microscopy

1986 ◽  
Vol 94 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Roberto Gamoletti ◽  
Paola Poggi ◽  
Mario Sanna ◽  
Carlo Zini
Keyword(s):  
Electron Microscopy ◽  
Middle Ear ◽  
Goblet Cells ◽  
Secretory Cells ◽  
Canal Wall ◽  
Ciliated Cells ◽  
Middle Ear Mucosa ◽  
Morphologic Characteristics ◽  
Transmission Electron ◽  
Ultrastructural Appearance

The ultrastructural appearance of the regenerated middle ear mucosa—found at the second operation of staged intact canal wall tympanoplasty (ICWT) with mastoidectomy—has been evaluated with the transmission electron microscope. The regenerated epithelium showed all the morphologic characteristics of the normal middle ear mucosa: ciliated cells, noncillated cells, and secretory cells. All of these (Including goblet cells) have been found in the specimens. It is concluded that a normal middle ear mucosa regenerates to cover all denuded bone surfaces after the first operation of staged ICWT with mastoidectomy, when silicone rubber sheeting has been used to prevent adhesions and maintain an air-containing middle ear space.

scholarly journals Regeneratad Human Middle Ear Mucosa in Tympanoplasty

1979 ◽  
Vol 72 (12) ◽  
pp. 1647-1654
Author(s):  
Eiji Yumoto ◽  
Naoaki Yanagihara
Keyword(s):  
Middle Ear ◽  
Middle Ear Mucosa ◽  
Human Middle Ear

Lactate Dehydrogenase Activity and Isoenzyme Patterns in Serous Middle Ear Effusions

1973 ◽  
Vol 82 (2) ◽  
pp. 192-195 ◽  
Author(s):  
S. K. Juhn ◽  
John S. Huff ◽  
Michael M. Paparella
Keyword(s):  
Lactate Dehydrogenase ◽  
Otitis Media ◽  
Middle Ear ◽  
Biochemical Characterization ◽  
Middle Ear Mucosa ◽  
Lactate Dehydrogenase Activity ◽  
Intracellular Enzyme ◽  
Ldh Activity ◽  
Serous Otitis Media ◽  
Isoenzyme Patterns

Biochemical characteristics of middle ear effusions (MEE) should provide a better understanding of the etiopathogenesis of serous otitis media. In order to develop another parameter for the biochemical characterization of the MEE, lactate dehydrogenase (LDH) and LDH isoenzyme patterns in the serous middle ear effusion and serum from 20 patients with serous otitis media were compared. The LDH activity was measured by the usual spectrophotometric method. The isoenzyme patterns were compared on electropherograms using cellulose polyacetate strips. The LDH activity in MEE was significantly higher (P < 0.001) than it was in serum. Fractions of isoenzymes 1 and 2 were each smaller in MEE than in serum. Isoenzymes 4 and 5 have a significantly higher (P < 0.001) fraction in MEE than in serum. Since LDH is an intracellular enzyme and middle ear mucosa is reported to have high content of isoenzymes 4 and 5, the inflammatory changes in the middle ear mucosa which may release intracellular LDH, are suggested as the cause of both higher activity of LDH and the higher fractions of isoenzymes 4 and 5 in MEE than those in the serum.

Localization of Prostaglandin-Forming Cyclooxygenase in Middle Ear and External Canal Tissue

1983 ◽  
Vol 91 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Timothy T.K. Jung ◽  
S.K. Juhn ◽  
Alfred F. Michael
Keyword(s):  
Endothelial Cells ◽  
Epithelial Cells ◽  
Middle Ear ◽  
Mononuclear Leukocytes ◽  
Middle Ear Effusion ◽  
Middle Ear Mucosa ◽  
Immunohistochemical Technique ◽  
External Canal ◽  
Human Middle Ear ◽  
Ear Effusion

Prostaglandins were found in the middle ear effusion and may play an important role in inflammation and bone resorption occurring in otitis media. With the use of an immunohistochemical technique, prostaglandin cyclooxygenase was localized in epithelial cells of human skin, endothelial cells of arterioles, mononuclear leukocytes and fibroblasts of human middle ear mucosa and granulation tissue, epithelial cells of chinchilla middle ear mucosa, and the external auditory canal skin.

Basement Membrane in Middle Ear Cholesteatoma Immunohistochemical and Ultrastructural Observations

1996 ◽  
Vol 105 (10) ◽  
pp. 804-810 ◽  
Author(s):  
Jesus Bujía ◽  
Anja Holly ◽  
Holger Sudhoff ◽  
Gerd Borkowski ◽  
Henning Hildmann ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Middle Ear ◽  
Collagen Type ◽  
Collagen Type Iv ◽  
Middle Ear Mucosa ◽  
Type Iv ◽  
Middle Ear Cholesteatoma ◽  
Human Middle Ear ◽  
Cell Matrix Interactions ◽  
Collagen Type Vii

We investigated the distribution of basement membrane zone (BMZ) components collagen type IV, collagen type VII, and fibronectin in human middle ear cholesteatoma, auditory meatal skin, and middle ear mucosa using both immunohistochemical and ultrastructural methods. Collagen type IV inununoreactivity of skin and middle ear mucosa is continuous in the BMZ, whereas cholesteatoma frequently showed absent immunoreactivity or focal discontinuities. Collagen type VII inununoreactivity is detected similarly within the BMZ of cholesteatoma and skin. Fibronectin immunoreactivity is observed within the dermoepithelial junction of skin and middle ear mucosa. In cholesteatoma, however, fibronectin immunoreactivity is markedly increased within the extrinsic BMZ and the subepithelial connective tissue. The ultrastructural arrangement of the BMZ of cholesteatoma is like that of skin; however, it exhibits distinct alterations of the lamina fibroreticularis and lamina densa. Our results outline cholesteatoma as a disease with disturbed cell matrix interactions analogous to those of wound reepithelialization.

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