Text Error in: Neonatal Sepsis: Looking Beyond the Blood Culture: Evaluation of a Study of Universal Primer Polymerase Chain Reaction for Identification of Neonatal Sepsis

2009 ◽  
Vol 163 (4) ◽  
pp. 348
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Neonatal Sepsis ◽  
Chain Reaction ◽  
Universal Primer ◽  
Polymerase Chain ◽  
Primer Polymerase Chain Reaction

Diagnosis of neonatal sepsis using 16S rRNA polymerase chain reaction

2017 ◽  
Vol 47 (4) ◽  
pp. 336-339 ◽  
Author(s):  
Harish Punia ◽  
Geeta Gathwala ◽  
Dhara B Dhaulakhandi ◽  
Mohammed Aamir
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
16S Rrna ◽  
Neonatal Sepsis ◽  
Predictive Value ◽  
Tertiary Care ◽  
College Teaching ◽  
Turnaround Time ◽  
Chain Reaction ◽  
Polymerase Chain

The gold standard for detecting bacterial sepsis is blood culture. However, the sensitivity of blood culture is low and the results take 48–72 h. Molecular assays for the detection of bacterial DNA permit early detection of a bacterial cause as the turnaround time is 6–8 h. We undertook an evaluation of the performance of universal bacterial primer (16S rRNA) polymerase chain reaction (PCR) in the diagnosis of neonatal sepsis at a tertiary care medical college teaching hospital. 16S rRNA PCR was positive in all cases of blood culture proven sepsis. PCR revealed 95.6% sensitivity, 100% specificity, 100% positive predictive value and 91.2% negative predictive value and so appears to be a useful tool for the early diagnosis of bacterial neonatal sepsis.

scholarly journals Validation of polymerase chain reaction in experimental sepsis diagnosis beyond blood culture

Critical Care ◽  
2008 ◽  
Vol 12 (Suppl 5) ◽  
pp. P47
Author(s):  
Marcello Ruiz-Silva ◽  
Derci Sa-Filho ◽  
Marcos Caseiro ◽  
Ivan Koh
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Experimental Sepsis ◽  
Chain Reaction ◽  
Sepsis Diagnosis ◽  
Polymerase Chain

scholarly journals Polymerase Chain Reaction and blood culture for diagnosis of canine sepsis

2018 ◽  
Vol 48 (6) ◽  
Author(s):  
Marcelo Marques da Silveira ◽  
Stéfhano Luis Cândido ◽  
Karin Rinaldi dos Santos ◽  
Maerle Oliveira Maia ◽  
Roberto Lopes de Souza ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Diagnostic Value ◽  
Turnaround Time ◽  
Diagnostic Techniques ◽  
Pcr Analysis ◽  
Chain Reaction ◽  
Suspected Sepsis ◽  
Fungal Dna ◽  
Polymerase Chain

ABSTRACT: Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%) animals, whereas fungal DNA was present in one (1.1%) sample. Our results showed that PCR-based testing has important diagnostic value for canine blood infections because it has a shorter turnaround time and higher sensitivity than traditional blood culture.

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