scholarly journals Geochemistry and microbial community composition across a range of acid mine drainage impact and implications for the Neoarchean-Paleoproterozoic transition

2017 ◽  
Vol 122 (6) ◽  
pp. 1404-1422 ◽  
Author(s):  
Jeff R. Havig ◽  
Christen Grettenberger ◽  
Trinity L. Hamilton
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Community Composition ◽  
Mine Drainage ◽  
Microbial Community Composition ◽  
Acid Mine

scholarly journals Fine-scale spatial patterns in microbial community composition in an acid mine drainage

2017 ◽  
Vol 93 (10) ◽  
Author(s):  
Jie-Liang Liang ◽  
Xiao-Jing Li ◽  
Hao-Yue Shu ◽  
Pandeng Wang ◽  
Jia-Liang Kuang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Community Composition ◽  
Spatial Patterns ◽  
Mine Drainage ◽  
Microbial Community Composition ◽  
Fine Scale ◽  
Acid Mine

scholarly journals Passive Treatment for Acid Mine Drainage Partially Restores Microbial Community Structure in Different Stream Habitats

Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3300
Author(s):  
Alexis N. Neff ◽  
Dean M. DeNicola ◽  
Chris Maltman
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Acid Mine Drainage ◽  
Microbial Communities ◽  
Community Composition ◽  
Microbial Community Structure ◽  
Mine Drainage ◽  
Microbial Community Composition ◽  
Taxonomic Composition ◽  
Passive Treatment

The assessment of the degree to which biological communities in streams impaired by acid mine drainage (AMD) are restored by passive treatment has focused primarily on eukaryotic-cell organisms and microbial processes. The responses of microbial community structure to passive treatment have received much less attention, even though functional processes such as nutrient cycling and organic matter decomposition depend on taxonomic composition. Our objective was to determine the degree to which passive treatment restored microbial communities in three types of habitats: aqueous, leaf, and sediment. To assess their recovery, we compared the community composition in these habitats based on 16S rRNA gene sequencing at three different stream sites: an untreated AMD site (U), a remediated site below AMD passive treatment (T), and an unimpaired reference site (R). The acidity, conductivity, and soluble metal concentrations at T were found to be elevated compared to R, but generally 1–2 orders of magnitude less than at U. Microbial community composition was found to be synergistically affected by habitat type and AMD impact, with the similarity among communities in the three habitats increasing with the severity of the AMD. Sediment- and leaf-associated microbial communities at U were characterized by taxa that are tolerant to severe AMD. The absence of the nitrogen oxidizing bacterium Nitrospira in sediment communities at T and U was found to correspond to higher NH4+ concentrations compared to R, possibly because of the presence of iron oxyhydroxide precipitate. In contrast, the microbial composition was found to be similar between the T and R sites for both aqueous and leaf communities, indicating that passive treatment was more able to restore these communities to the reference condition than sediment communities. The remediation of AMD streams should consider the habitat-specific responses of microbial community composition and be guided by future studies that empirically couple changes in taxonomic composition to measured functional processes.

Effect of hydraulic retention time on microbial community in biochemical passive reactors during treatment of acid mine drainage

2018 ◽  
Vol 247 ◽  
pp. 624-632 ◽  
Author(s):  
Yaneth Vasquez ◽  
Maria C. Escobar ◽  
Johan S. Saenz ◽  
Maria F. Quiceno-Vallejo ◽  
Carmen M. Neculita ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Mine Drainage ◽  
Acid Mine

Assessing arsenic bioremediation potential of epilithic biofilms affected by acid-mine drainage

2020 ◽  
Author(s):  
Sarah Zecchin ◽  
Nicoletta Guerrieri ◽  
Evelien Jongepier ◽  
Leonardo Scaglioni ◽  
Gigliola Borgonovo ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Heterotrophic Bacteria ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Sampling Point ◽  
Epilithic Biofilm ◽  
Acid Mine ◽  
Epilithic Biofilms

<p>Arsenic is a toxic but naturally abundant metalloid that globally leads to contamination in groundwater and soil, exposing millions of people to cancer and other arsenic-related diseases. In several areas in Northern Italy arsenic in soil and water exceeds law limits (20 mg kg<sup>-1</sup> and 10 mg L<sup>-1</sup>, respectively), due to both the mineralogy of bedrock and former mining activities. The Rio Rosso stream, located in the Anzasca Valley (Piedmont) is heavily affected by an acid mine drainage originated from an abandoned gold mine. Arsenic, together with other heavy metals, is transferred by the stream to the surrounding area. The stream is characterized by the presence of an extensive reddish epilithic biofilm at the opening of the mine and on the whole contaminated waterbed.</p> <p>The aim of this study was to characterize the mechanisms allowing the biotic fraction of this biofilm to cope with extreme arsenic concentrations. The composition and functionality of the microbial communities constituting the epilithic biofilms sampled in the close proximity and downstream the mine were unraveled by 16S rRNA genes and shotgun Illumina sequencing in relation to the extreme physico-chemical parameters. In parallel, autotrophic and heterotrophic microbial populations were characterized <em>in vivo</em> by enrichment cultivation and isolated strains were tested for their ability to perform arsenic redox transformation.</p> <p>Preliminary analyses indicated that the biofilm accumulated arsenic in the order of 6 · 10<sup>3</sup> mg kg<sup>-1</sup>, in contrast to 0.14 mg L<sup>-1</sup>, measured in the surrounding water. The main chemical parameter affecting the composition of the microbial community was the pH, being 2 next to the mine and 6.7 in the downstream sampling point. In both sampling sites iron- and sulfur-cycling microorganisms were retrieved by both cultivation and molecular methods. However, the diversity of the microbial community living next to the mine was significantly lower with respect to the community developed downstream. In the latter, autotrophic <em>Cyanobacteria</em> belonging to the species <em>Tychonema</em> were the dominant taxa. A complete arsenic cycle was shown to occur, with heterotrophic bacteria mainly responsible for arsenate reduction and autotrophic bacteria performing arsenite  oxidation.</p> <p>These observations indicate that the epilithic biofilm living in the Rio Rosso stream represents a peculiar ecosystem where microorganisms cope with metalloid toxicity likely using diverse mechanisms. Such microbial metabolic properties might be exploited in bioremediation strategies applied in arsenic-contaminated environments.</p>

A novel uncultured bacterium of the family Gallionellaceae: Description and genome reconstruction based on metagenomic analysis of microbial community in acid mine drainage

Microbiology ◽  
2016 ◽  
Vol 85 (4) ◽  
pp. 449-461 ◽  
Author(s):  
V. V. Kadnikov ◽  
D. A. Ivasenko ◽  
A. V. Beletskii ◽  
A. V. Mardanov ◽  
E. V. Danilova ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Metagenomic Analysis ◽  
Uncultured Bacterium ◽  
Genome Reconstruction ◽  
Acid Mine ◽  
The Family

Characterization of the microbial acid mine drainage microbial community using culturing and direct sequencing techniques

2013 ◽  
Vol 93 (2) ◽  
pp. 108-115 ◽  
Author(s):  
Ryan R. Auld ◽  
Maxine Myre ◽  
Nadia C.S. Mykytczuk ◽  
Leo G. Leduc ◽  
Thomas J.S. Merritt
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Direct Sequencing ◽  
Acid Mine
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