scholarly journals Performance of microbial community dominated by Bacillus spp. in acid mine drainage remediation systems: A focus on the high removal efficiency of SO42-, Al3+, Cd2+, Cu2+, Mn2+, Pb2+, and Sr2+

Heliyon ◽  
2021 ◽  
pp. e07241
Author(s):  
Enoch A. Akinpelu ◽  
Seteno K.O. Ntwampe ◽  
Elvis Fosso-Kankeu ◽  
Felix Nchu ◽  
Justine O. Angadam
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Removal Efficiency ◽  
Mine Drainage ◽  
Acid Mine ◽  
Bacillus Spp

Effect of hydraulic retention time on microbial community in biochemical passive reactors during treatment of acid mine drainage

2018 ◽  
Vol 247 ◽  
pp. 624-632 ◽  
Author(s):  
Yaneth Vasquez ◽  
Maria C. Escobar ◽  
Johan S. Saenz ◽  
Maria F. Quiceno-Vallejo ◽  
Carmen M. Neculita ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Mine Drainage ◽  
Acid Mine

scholarly journals Enhanced Monovalent Cation Biomineralization Ability by Quartz Sand for Effective Removal of Soluble Iron in Simulated Acid Mine Drainage

Water ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 732
Author(s):  
Heru Wang ◽  
Mengying Li ◽  
Yongwei Song
Keyword(s):  
Heavy Metals ◽  
Acid Mine Drainage ◽  
Removal Efficiency ◽  
Quartz Sand ◽  
Mine Drainage ◽  
Monovalent Cation ◽  
Iron Minerals ◽  
Precipitation Efficiency ◽  
Acid Mine ◽  
Effective Removal

Acid mine drainage (AMD) is characterized by low pH, high soluble Fe, and heavy metal concentrations. Conventional lime neutralization produces large amounts of Fe(OH)2 and Fe(OH)3, which complicate subsequent disposal. Secondary iron minerals synthesized by biomineralization can reduce the concentration of soluble Fe in addition to adsorbing and removing heavy metals in AMD. Therefore, an appropriate method for improving the precipitation efficiency of Fe is urgently needed for AMD treatment. Using simulated AMD, this work analyzes the influence of quartz sand (40 g/L) on the Fe2+ oxidation and total Fe deposition efficiencies, as well as the phases of secondary iron minerals in an Acidithiobacillus ferrooxidans system including K+, Na+, or NH4+ (53.3 mmol/L). Quartz sand had no significant effect on Fe2+ oxidation and 160 mmol/L Fe2+ was completely oxidized by A. ferrooxidans in 168 h, but contributed to the oxidized product (Fe3+) mineralization, improving the total Fe removal efficiency in simulated AMD. Compared with treatments involving K+ or Na+ alone, quartz sand improved the total Fe precipitation efficiency by 26.6% or 30.2%, respectively. X-ray diffraction showed that quartz sand can promote the transformation of the biomineralization pathway from schwertmannite to jarosite with higher yields, which is important for improving the removal efficiency of heavy metals in AMD.

Assessing arsenic bioremediation potential of epilithic biofilms affected by acid-mine drainage

2020 ◽  
Author(s):  
Sarah Zecchin ◽  
Nicoletta Guerrieri ◽  
Evelien Jongepier ◽  
Leonardo Scaglioni ◽  
Gigliola Borgonovo ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Heterotrophic Bacteria ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Sampling Point ◽  
Epilithic Biofilm ◽  
Acid Mine ◽  
Epilithic Biofilms

<p>Arsenic is a toxic but naturally abundant metalloid that globally leads to contamination in groundwater and soil, exposing millions of people to cancer and other arsenic-related diseases. In several areas in Northern Italy arsenic in soil and water exceeds law limits (20 mg kg<sup>-1</sup> and 10 mg L<sup>-1</sup>, respectively), due to both the mineralogy of bedrock and former mining activities. The Rio Rosso stream, located in the Anzasca Valley (Piedmont) is heavily affected by an acid mine drainage originated from an abandoned gold mine. Arsenic, together with other heavy metals, is transferred by the stream to the surrounding area. The stream is characterized by the presence of an extensive reddish epilithic biofilm at the opening of the mine and on the whole contaminated waterbed.</p> <p>The aim of this study was to characterize the mechanisms allowing the biotic fraction of this biofilm to cope with extreme arsenic concentrations. The composition and functionality of the microbial communities constituting the epilithic biofilms sampled in the close proximity and downstream the mine were unraveled by 16S rRNA genes and shotgun Illumina sequencing in relation to the extreme physico-chemical parameters. In parallel, autotrophic and heterotrophic microbial populations were characterized <em>in vivo</em> by enrichment cultivation and isolated strains were tested for their ability to perform arsenic redox transformation.</p> <p>Preliminary analyses indicated that the biofilm accumulated arsenic in the order of 6 · 10<sup>3</sup> mg kg<sup>-1</sup>, in contrast to 0.14 mg L<sup>-1</sup>, measured in the surrounding water. The main chemical parameter affecting the composition of the microbial community was the pH, being 2 next to the mine and 6.7 in the downstream sampling point. In both sampling sites iron- and sulfur-cycling microorganisms were retrieved by both cultivation and molecular methods. However, the diversity of the microbial community living next to the mine was significantly lower with respect to the community developed downstream. In the latter, autotrophic <em>Cyanobacteria</em> belonging to the species <em>Tychonema</em> were the dominant taxa. A complete arsenic cycle was shown to occur, with heterotrophic bacteria mainly responsible for arsenate reduction and autotrophic bacteria performing arsenite  oxidation.</p> <p>These observations indicate that the epilithic biofilm living in the Rio Rosso stream represents a peculiar ecosystem where microorganisms cope with metalloid toxicity likely using diverse mechanisms. Such microbial metabolic properties might be exploited in bioremediation strategies applied in arsenic-contaminated environments.</p>

A novel uncultured bacterium of the family Gallionellaceae: Description and genome reconstruction based on metagenomic analysis of microbial community in acid mine drainage

Microbiology ◽  
2016 ◽  
Vol 85 (4) ◽  
pp. 449-461 ◽  
Author(s):  
V. V. Kadnikov ◽  
D. A. Ivasenko ◽  
A. V. Beletskii ◽  
A. V. Mardanov ◽  
E. V. Danilova ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Metagenomic Analysis ◽  
Uncultured Bacterium ◽  
Genome Reconstruction ◽  
Acid Mine ◽  
The Family

Characterization of the microbial acid mine drainage microbial community using culturing and direct sequencing techniques

2013 ◽  
Vol 93 (2) ◽  
pp. 108-115 ◽  
Author(s):  
Ryan R. Auld ◽  
Maxine Myre ◽  
Nadia C.S. Mykytczuk ◽  
Leo G. Leduc ◽  
Thomas J.S. Merritt
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Direct Sequencing ◽  
Acid Mine

scholarly journals Fine-scale spatial patterns in microbial community composition in an acid mine drainage

2017 ◽  
Vol 93 (10) ◽  
Author(s):  
Jie-Liang Liang ◽  
Xiao-Jing Li ◽  
Hao-Yue Shu ◽  
Pandeng Wang ◽  
Jia-Liang Kuang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Community Composition ◽  
Spatial Patterns ◽  
Mine Drainage ◽  
Microbial Community Composition ◽  
Fine Scale ◽  
Acid Mine

scholarly journals 16S rRNA Gene-Based Profiling of the Microbial Community in an Acid Mine Drainage Fe Precipitate at Libiola Mine (Liguria, Italy)

Minerals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1064
Author(s):  
Sirio Consani ◽  
Stefano Ghignone ◽  
Marina Pozzolini ◽  
Marco Giovine ◽  
Luigi Vezzulli ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Bacterial Species ◽  
Ecological Niches ◽  
Rrna Gene ◽  
Acid Mine ◽  
Starting Point ◽  
Wide Range ◽  
Libiola Mine

Acid mine drainage (AMD) is a common environmental problem in many sulphide mines worldwide, and it is widely accepted that the microbial community plays a major role in keeping the process of acid generation active. The aim of this work is to describe, for the first time, the microbial community thriving in goethite and jarosite Fe precipitates from the AMD of the Libiola mine. The observed association is dominated by Proteobacteria (>50%), followed by Bacteroidetes (22.75%), Actinobacteria (7.13%), Acidobacteria (5.79%), Firmicutes (2.56%), and Nitrospirae (1.88%). Primary producers seem to be limited to macroalgae, with chemiolithotrophic strains being almost absent. A phylogenetic analysis of bacterial sequences highlighted the presence of heterotrophic bacteria, including genera actively involved in the AMD Fe cycle and genera (such as Cytophaga and Flavobacterium) that are able to reduce cellulose. The Fe precipitates constitute a microaerobic and complex environment in which many ecological niches are present, as proved by the wide range of bacterial species observed. This study is the first attempt to quantitatively characterize the microbial community of the studied area and constitutes a starting point to learn more about the microorganisms thriving in the AMD of the Libiola mine, as well as their potential applications.

scholarly journals Bioremediation of acid mine drainage and crude contaminated soils

2020 ◽  
Author(s):  
◽  
Yusuf Makarfi Isa
Keyword(s):  
Acid Mine Drainage ◽  
Crude Oil ◽  
Removal Efficiency ◽  
Contaminated Soils ◽  
Mine Drainage ◽  
Metal Removal ◽  
Air Injection ◽  
Treatment System ◽  
Acid Mine ◽  
Removal Efficiencies

Pollution is one of the greatest ills plaguing the existence of the ecosystem which could lead to the annihilation of terrestrial and aquatic habitat if not remedied. Acid mine drainage (AMD) and crude oil are among the major land and water pollutants cause by industrial and human activities. The constant exploration, mining, and processing of mineral resources and prevalent use of petroleum products for economic purposes have contributed to contamination of soil and proximate water bodies which results in environmental degradation; thus, remediation becomes necessary. The treatment of AMD contaminated soils using the conventional methods has some room for improvement to meet the remediation purpose. Bioremediation technology provides a sustainable and eco-friendly approach to the treatment of contaminants. This study aims to evaluate the performance of different potential bioremediation techniques and conduct a comparative analysis of these methods for the treatment of AMD and crude oil-contaminated soils. The treatment approach for both pollutants comprises of soils separately contaminated with AMD and crude oil before the application of bioremediation techniques. For the biostimulation study, contaminated soils were amended with varying ratios of the brewery or municipal wastewaters (BWW and MWW), while the bioventing (BVT) treatment involved wastewater amendment and supply of atmospheric air from the vadose zone at 3L/min at 30 minutes intervals every 48 hours. The bacteria strain Pseudomonas aeruginosa ATCC 15442 used for the study which was inoculated at 5%(w/w) was cultured in two different media for respective treatments and wastewater was amended as an extra energy source for bioaugmentation (BAU) study while Bioattenuation (BAT) which received no amendment was used as a control treatment for the study. The treatments were conducted in plastic bioreactors under mesophilic conditions for 28 days and samples were collected from each treatment system on weekly basis to analyse for sulfate, heavy metals, and total petroleum hydrocarbon (TPH) reduction. The result of the study showed that the amendment of contaminated soils with wastewater increased alkalinity in the system which enhanced microbial activities for effective remediation which recorded 52.43 and 51.23% average TPH and metal removal efficiency for the BSTc treatment. Also, the combined application of bioremediation techniques was more effective than single application as the introduction of oxygen into the treatment system with wastewater amendment increased the TPH and metal removal efficiency by an average of 12.98 and 13.17% respectively but efforts to enhance sulfate removal by air-injection (BVTa) proved abortive with 17.20 and 14.67% removal efficiencies less than BSTa and BAUa respectively as sulfate-reducing bacteria thrive in an anaerobic environment. However, P. aeruginosa ATCC 15442 adopts the sorption process in the reduction of hydrocarbon and metal toxicity with 42.02 and 41.81% average removal efficiencies respectively and the amendment extra nutrient (wastewater) increased the removal efficiency of these pollutants by 25.24 and 16.23% respectively. The results of the study inferred that wastewater (BWW and MWW), air-injection and P. aeruginosa ATCC 15442 showed great potentials in the degradation and removal of TPH, metals and sulfate contaminants, hence, can serve as a viable strategy for the remediation of AMD and crude oil polluted soils while improving waste management and amelioration of pollution aftermath faced by communities involved in mining and oil production and/or processing. There is a need for optimization to ensure effective remediation while further study is required to validate large scale application.

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