Experimental Protocol and Testing Environment

We have investigated the effect of integral membrane proteins upon the fracturing of frozen lipid bilayers. This investigation has been part of an effort to develop freeze fracture labeling techniques and to assess the possible breakage of covalent protein bonds during the freeze fracture process. We have developed an experimental protocol utilizing lectin affinity columns which should detect small amounts of covalent bond breakage during the fracture of liposomes containing purified (1) glycophorin (a transmembrane glycoprotein of human erythrocyte membranes). To fracture liposomes in bulk, frozen liposomes are ground repeatedly under liquid nitrogen. Failure to detect any significant covalent bond breakage (contrary to (2)) led us to question the effectiveness of our grinding procedure in fracturing and splitting lipid bilayers.

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Experimental Protocol for Study and Evaluation of Post-Surgical Perineural Scar

Journal of Reconstructive Microsurgery ◽

10.1055/s-0034-1373907 ◽

2014 ◽

Vol 30 (S 01) ◽

Author(s):

Crosio Alessandro ◽

Pierluigi Tos ◽

Mario Cherubino ◽

Matteo Izzo ◽

Stefano Geuna ◽

...

Keyword(s):

Experimental Protocol

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On the Reliability of the Use of Heparin Immobilized on Agarose for the Study of the Interactions among Heparin, Thrombin and Antithrombin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657222 ◽

1982 ◽

Vol 48 (01) ◽

pp. 084-086

Author(s):

Wayne W Fish ◽

Ingemar Björk

Keyword(s):

Experimental Approach ◽

Antithrombin Iii ◽

Chromogenic Substrate ◽

Experimental Protocol ◽

Appreciable Difference ◽

Thrombin Inhibition ◽

Highly Sensitive ◽

Inhibition Of Thrombin

SummaryThe extent of inhibition of thrombin was re-examined as a consequence of the sequence of addition of thrombin and antithrombin III to a column of heparin immobilized on agarose. With the use of pure enzyme, pure inhibitor, and a highly sensitive chromogenic substrate, no appreciable difference in the extent of thrombin inhibition was observed between the two sequences of addition. These observations, together with a demonstrated sensitivity of the method to variations in experimental protocol, challenge the conclusions reached in an earlier work (Hatton and Regoeczi, Thromb. Res. 1977; 10:645) which utilized this experimental approach but which employed larger quantities of reactants and a less sensitive substrate.

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An Experimental Protocol for the Study of Brain Functional Magnetic Resonance Imaging in Female With Urgent Urinary Incontinence

Case Medical Research ◽

10.31525/ct1-nct04271852 ◽

2020 ◽

Author(s):

Keyword(s):

Magnetic Resonance Imaging ◽

Urinary Incontinence ◽

Magnetic Resonance ◽

Functional Magnetic Resonance Imaging ◽

Functional Magnetic Resonance ◽

Resonance Imaging ◽

Experimental Protocol

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Topological isoconductance signatures in Majorana nanowires

Scientific Reports ◽

10.1038/s41598-021-96415-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

L. S. Ricco ◽

J. E. Sanches ◽

Y. Marques ◽

M. de Souza ◽

M. S. Figueira ◽

...

Keyword(s):

Quantum Dot ◽

Transport Properties ◽

Spin Polarization ◽

Bound States ◽

Spin Asymmetry ◽

Experimental Protocol ◽

Zero Bias ◽

Topological Superconductor ◽

Hybrid Device ◽

Majorana Bound States

AbstractWe consider transport properties of a hybrid device composed by a quantum dot placed between normal and superconducting reservoirs, and coupled to a Majorana nanowire: a topological superconducting segment hosting Majorana bound states (MBSs) at the opposite ends. It is demonstrated that if highly nonlocal and nonoverlapping MBSs are formed in the system, the zero-bias Andreev conductance through the dot exhibits characteristic isoconductance profiles with the shape depending on the spin asymmetry of the coupling between the dot and the topological superconductor. Otherwise, for overlapping MBSs with less degree of nonlocality, the conductance is insensitive to the spin polarization and the isoconductance signatures disappear. This allows to propose an alternative experimental protocol for probing the nonlocality of the MBSs in Majorana nanowires.

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Synthesis and characterization of analcime (ANA) zeolite using a kaolinitic rock

Scientific Reports ◽

10.1038/s41598-021-92862-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Daniela Novembre ◽

Domingo Gimeno

Keyword(s):

Ambient Pressure ◽

Added Value ◽

Emission Spectrometry ◽

Quantitative Phase Analysis ◽

Crystallization Time ◽

Experimental Protocol ◽

Economic Point ◽

Cell Parameters ◽

Synthesis Protocol

AbstractAnalcime is nowadays an important component in dental porcelain systems, in heterogeneous catalysis, in the nanoelectronic field, in selective adsorption and in stomatology (dental filling and prosthesis). Analcime synthesis from an impure, silica-rich kaolinite rock coming from Romana (Sassari, Italy) is here presented. A synthesis protocol is proposed that aims to make an improvement of synthesis conditions compared to the past. The hydrothermal treatment is in fact here achieved without aging times and without the use of sodium silicate or other additional silica source reported in the literature. Lower calcination temperature, synthesis temperature and crystallization time are verified in this work. The kaolin is subjected to calcination at the temperature of 650 °C and then mixed with NaOH. The experiment is performed at ambient pressure and 170 ± 0.1 °C. The degree of purity of analcime is calculated in 97.57% at 10 h. Analcime is characterized by X-ray diffraction, infrared spectroscopy, scanning electron microscopy, inductively coupled plasma optical emission spectrometry and thermal analysis. Density is also calculated. Cell parameters and the amount of amorphous phase in the synthesis powders is estimated with quantitative phase analysis using the combined Rietveld and reference intensity ratio methods. The experimental conditions make the synthesis protocol particularly attractive from an economic point of view. Also this work does not use a commercial kaolin but silica-rich impure kaolinitic rock from a disused quarry. This further reduces the costs of the experimental protocol. It also gives the protocol an added value, as the synthesis of a useful mineral is obtained through the valorization of an otherwise unused georesource. Both chemical and physical characterization of analcime is satisfactory making the experimental protocol very promising for an industrial transfer.

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ESRFuzzer: an enhanced fuzzing framework for physical SOHO router devices to discover multi-Type vulnerabilities

Cybersecurity ◽

10.1186/s42400-021-00091-9 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Yu Zhang ◽

Wei Huo ◽

Kunpeng Jian ◽

Ji Shi ◽

Longquan Liu ◽

...

Keyword(s):

Web Server ◽

Test Cases ◽

Communication Model ◽

Home Office ◽

Security Vulnerabilities ◽

Testing Environment ◽

Semantic Models ◽

Recovery Mechanisms ◽

General Mode ◽

Soho Router

AbstractSOHO (small office/home office) routers provide services for end devices to connect to the Internet, playing an important role in cyberspace. Unfortunately, security vulnerabilities pervasively exist in these routers, especially in the web server modules, greatly endangering end users. To discover these vulnerabilities, fuzzing web server modules of SOHO routers is the most popular solution. However, its effectiveness is limited due to the lack of input specification, lack of routers’ internal running states, and lack of testing environment recovery mechanisms. Moreover, existing works for device fuzzing are more likely to detect memory corruption vulnerabilities.In this paper, we propose a solution ESRFuzzer to address these issues. It is a fully automated fuzzing framework for testing physical SOHO devices. It continuously and effectively generates test cases by leveraging two input semantic models, i.e., KEY-VALUE data model and CONF-READ communication model, and automatically recovers the testing environment with power management. It also coordinates diversified mutation rules with multiple monitoring mechanisms to trigger multi-type vulnerabilities. With the guidance of the two semantic models, ESRFuzzer can work in two ways: general mode fuzzing and D-CONF mode fuzzing. General mode fuzzing can discover both issues which occur in the CONF and READ operation, while D-CONF mode fuzzing focus on the READ-op issues especially missed by general mode fuzzing.We ran ESRFuzzer on 10 popular routers across five vendors. In total, it discovered 136 unique issues, 120 of which have been confirmed as 0-day vulnerabilities we found. As an improvement of SRFuzzer, ESRFuzzer have discovered 35 previous undiscovered READ-op issues that belong to three vulnerability types, and 23 of them have been confirmed as 0-day vulnerabilities by vendors. The experimental results show that ESRFuzzer outperforms state-of-the-art solutions in terms of types and number of vulnerabilities found.

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CORRELATING DESIGN PERFORMANCE TO EEG ACTIVATION: EARLY EVIDENCE FROM EXPERIMENTAL DATA

Proceedings of the Design Society ◽

10.1017/pds.2021.77 ◽

2021 ◽

Vol 1 ◽

pp. 771-780

Author(s):

Shumin Li ◽

Niccolò Becattini ◽

Gaetano Cascini

Keyword(s):

Idea Generation ◽

Occipital Area ◽

Experimental Protocol ◽

Brain Response ◽

Design Task ◽

Early Evidence ◽

Eeg Data ◽

Eeg Activation ◽

Level Design ◽

Eeg Alpha

AbstractThis paper presents an EEG (Electroencephalography) study that explores correlations between the neurophysiological activations, the nature of the design task and its outputs. We propose an experimental protocol that covers several design-related tasks: including fundamental activities (e.g. idea generation and problem-solving) as well as more comprehensive task requiring the complex higher-level reasoning of designing. We clustered the collected data according to the characteristics of the design outcome and measured EEG alpha band activation during elementary and higher-level design task, whereas just the former yielded statistically significant different behaviour in the left frontal and occipital area. We also found a significant correlation between the ratings for elementary sketching task outcomes and EEG activation at the higher-level design task. These results suggested that EEG activation enables distinguishing groups according to their performance only for elementary tasks. However, this also suggests a potential application of EEG data on the elementary tasks to distinguish the designers' brain response during higher-level of design task.

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