Experimental Protocol for Study and Evaluation of Post-Surgical Perineural Scar

2014 ◽  
Vol 30 (S 01) ◽  
Author(s):  
Crosio Alessandro ◽  
Pierluigi Tos ◽  
Mario Cherubino ◽  
Matteo Izzo ◽  
Stefano Geuna ◽  
...  
Keyword(s):  
Experimental Protocol

Effect of Integral Proteins on Frozen Membrane Fracture

1980 ◽  
Vol 38 ◽  
pp. 756-759 ◽  
Author(s):  
S. Kirchanski ◽  
D. Branton
Keyword(s):  
Lipid Bilayers ◽  
Freeze Fracture ◽  
Covalent Bond ◽  
Erythrocyte Membranes ◽  
Glycophorin A ◽  
Experimental Protocol ◽  
Transmembrane Glycoprotein ◽  
Bond Breakage ◽  
Human Erythrocyte Membranes ◽  
Integral Proteins

We have investigated the effect of integral membrane proteins upon the fracturing of frozen lipid bilayers. This investigation has been part of an effort to develop freeze fracture labeling techniques and to assess the possible breakage of covalent protein bonds during the freeze fracture process. We have developed an experimental protocol utilizing lectin affinity columns which should detect small amounts of covalent bond breakage during the fracture of liposomes containing purified (1) glycophorin (a transmembrane glycoprotein of human erythrocyte membranes). To fracture liposomes in bulk, frozen liposomes are ground repeatedly under liquid nitrogen. Failure to detect any significant covalent bond breakage (contrary to (2)) led us to question the effectiveness of our grinding procedure in fracturing and splitting lipid bilayers.

On the Reliability of the Use of Heparin Immobilized on Agarose for the Study of the Interactions among Heparin, Thrombin and Antithrombin

1982 ◽  
Vol 48 (01) ◽  
pp. 084-086
Author(s):  
Wayne W Fish ◽  
Ingemar Björk
Keyword(s):  
Experimental Approach ◽  
Antithrombin Iii ◽  
Chromogenic Substrate ◽  
Experimental Protocol ◽  
Appreciable Difference ◽  
Thrombin Inhibition ◽  
Highly Sensitive ◽  
Inhibition Of Thrombin

SummaryThe extent of inhibition of thrombin was re-examined as a consequence of the sequence of addition of thrombin and antithrombin III to a column of heparin immobilized on agarose. With the use of pure enzyme, pure inhibitor, and a highly sensitive chromogenic substrate, no appreciable difference in the extent of thrombin inhibition was observed between the two sequences of addition. These observations, together with a demonstrated sensitivity of the method to variations in experimental protocol, challenge the conclusions reached in an earlier work (Hatton and Regoeczi, Thromb. Res. 1977; 10:645) which utilized this experimental approach but which employed larger quantities of reactants and a less sensitive substrate.

scholarly journals Topological isoconductance signatures in Majorana nanowires

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
L. S. Ricco ◽  
J. E. Sanches ◽  
Y. Marques ◽  
M. de Souza ◽  
M. S. Figueira ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Transport Properties ◽  
Spin Polarization ◽  
Bound States ◽  
Spin Asymmetry ◽  
Experimental Protocol ◽  
Zero Bias ◽  
Topological Superconductor ◽  
Hybrid Device ◽  
Majorana Bound States

AbstractWe consider transport properties of a hybrid device composed by a quantum dot placed between normal and superconducting reservoirs, and coupled to a Majorana nanowire: a topological superconducting segment hosting Majorana bound states (MBSs) at the opposite ends. It is demonstrated that if highly nonlocal and nonoverlapping MBSs are formed in the system, the zero-bias Andreev conductance through the dot exhibits characteristic isoconductance profiles with the shape depending on the spin asymmetry of the coupling between the dot and the topological superconductor. Otherwise, for overlapping MBSs with less degree of nonlocality, the conductance is insensitive to the spin polarization and the isoconductance signatures disappear. This allows to propose an alternative experimental protocol for probing the nonlocality of the MBSs in Majorana nanowires.

scholarly journals Synthesis and characterization of analcime (ANA) zeolite using a kaolinitic rock

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Daniela Novembre ◽  
Domingo Gimeno
Keyword(s):  
Ambient Pressure ◽  
Added Value ◽  
Emission Spectrometry ◽  
Quantitative Phase Analysis ◽  
Crystallization Time ◽  
Experimental Protocol ◽  
Economic Point ◽  
Cell Parameters ◽  
Synthesis Protocol

AbstractAnalcime is nowadays an important component in dental porcelain systems, in heterogeneous catalysis, in the nanoelectronic field, in selective adsorption and in stomatology (dental filling and prosthesis). Analcime synthesis from an impure, silica-rich kaolinite rock coming from Romana (Sassari, Italy) is here presented. A synthesis protocol is proposed that aims to make an improvement of synthesis conditions compared to the past. The hydrothermal treatment is in fact here achieved without aging times and without the use of sodium silicate or other additional silica source reported in the literature. Lower calcination temperature, synthesis temperature and crystallization time are verified in this work. The kaolin is subjected to calcination at the temperature of 650 °C and then mixed with NaOH. The experiment is performed at ambient pressure and 170 ± 0.1 °C. The degree of purity of analcime is calculated in 97.57% at 10 h. Analcime is characterized by X-ray diffraction, infrared spectroscopy, scanning electron microscopy, inductively coupled plasma optical emission spectrometry and thermal analysis. Density is also calculated. Cell parameters and the amount of amorphous phase in the synthesis powders is estimated with quantitative phase analysis using the combined Rietveld and reference intensity ratio methods. The experimental conditions make the synthesis protocol particularly attractive from an economic point of view. Also this work does not use a commercial kaolin but silica-rich impure kaolinitic rock from a disused quarry. This further reduces the costs of the experimental protocol. It also gives the protocol an added value, as the synthesis of a useful mineral is obtained through the valorization of an otherwise unused georesource. Both chemical and physical characterization of analcime is satisfactory making the experimental protocol very promising for an industrial transfer.

scholarly journals CORRELATING DESIGN PERFORMANCE TO EEG ACTIVATION: EARLY EVIDENCE FROM EXPERIMENTAL DATA

2021 ◽  
Vol 1 ◽  
pp. 771-780
Author(s):  
Shumin Li ◽  
Niccolò Becattini ◽  
Gaetano Cascini
Keyword(s):  
Idea Generation ◽  
Occipital Area ◽  
Experimental Protocol ◽  
Brain Response ◽  
Design Task ◽  
Early Evidence ◽  
Eeg Data ◽  
Eeg Activation ◽  
Level Design ◽  
Eeg Alpha

AbstractThis paper presents an EEG (Electroencephalography) study that explores correlations between the neurophysiological activations, the nature of the design task and its outputs. We propose an experimental protocol that covers several design-related tasks: including fundamental activities (e.g. idea generation and problem-solving) as well as more comprehensive task requiring the complex higher-level reasoning of designing. We clustered the collected data according to the characteristics of the design outcome and measured EEG alpha band activation during elementary and higher-level design task, whereas just the former yielded statistically significant different behaviour in the left frontal and occipital area. We also found a significant correlation between the ratings for elementary sketching task outcomes and EEG activation at the higher-level design task. These results suggested that EEG activation enables distinguishing groups according to their performance only for elementary tasks. However, this also suggests a potential application of EEG data on the elementary tasks to distinguish the designers' brain response during higher-level of design task.

scholarly journals Experimental protocol for the study of One-pot amination of Cyclohexanone-to-Secondary amines over Carbon-supported Pd

MethodsX ◽  
2021 ◽  
pp. 101406
Author(s):  
Luis E. Arteaga-Pérez ◽  
Raydel Manrique ◽  
Francisca Castillo-Puchi ◽  
Maray Ortega ◽  
Camila Bertiola ◽  
...  
Keyword(s):  
Secondary Amines ◽  
Experimental Protocol ◽  
One Pot ◽  
Supported Pd

Evaluating the Usability of University Waste-Sorting Systems

2020 ◽  
Vol 64 (1) ◽  
pp. 2046-2050
Author(s):  
Nathan Dumessa ◽  
Katie Lucaites ◽  
Hannah Solini ◽  
Jeremy Lopez ◽  
Paige Lawton ◽  
...  
Keyword(s):  
User Testing ◽  
Experimental Protocol ◽  
Clemson University ◽  
Human Centered Design ◽  
Testing Methodology ◽  
Design Practices ◽  
Competitive Price ◽  
Large Populations ◽  
New System

Recycling is a widely adopted practice that can reduce waste in landfills and increase profits for companies, corporations, and universities, such as Clemson, that sell recyclable materials. For these materials to be sold at a competitive price, however, they must be uncontaminated. This is a considerable barrier for Clemson University because users’ knowledge and experience with recycling do not align with Clemson University’s expected recycling practices. In an effort to bridge this gap, we followed human-centered design practices to generate and test prototypes of new system signage. We found that users were more accurate and confident in their sorting behavior when interacting with the new signage. Users also perceived the new signage as more usable than the old signage. In addition to these findings, the results of this research include a user testing methodology and experimental protocol that can be implemented in other contexts where large populations sort waste.

scholarly journals Long-Term Cryostorage of Mesenchymal Stem Cell-Containing Hybrid Hydrogel Scaffolds Based on Fibrin and Collagen

Gels ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 44
Author(s):  
Marfa N. Egorikhina ◽  
Yulia P. Rubtsova ◽  
Diana Ya. Aleynik
Keyword(s):  
Tissue Engineering ◽  
Proliferative Activity ◽  
Experimental Protocol ◽  
Hydrogel Scaffold ◽  
Hybrid Hydrogel ◽  
Hydrogel Scaffolds ◽  
High Viability ◽  
Scaffold Structure ◽  
Difficult Issue

The most difficult issue when using tissue engineering products is enabling the ability to store them without losing their restorative capacity. The numbers and viability of mesenchymal stem cells encapsulated in a hydrogel scaffold after cryostorage at −80 °C (by using, individually, two kinds of cryoprotectors—Bambanker and 10% DMSO (Dimethyl sulfoxide) solution) for 3, 6, 9, and 12 months were determined, with subsequent assessment of cell proliferation after 96 h. The analysis of the cellular component was performed using fluorescence microscopy and the two fluorochromes—Hoechst 3334 and NucGreenTM Dead 488. The experimental protocol ensured the preservation of cells in the scaffold structure, retaining both high viability and proliferative activity during storage for 3 months. Longer storage of scaffolds led to their significant changes. Therefore, after 6 months, the proliferative activity of cells decreased. Cryostorage of scaffolds for 9 months led to a decrease in cells’ viability and proliferative activity. As a result of cryostorage of scaffolds for 12 months, a decrease in viability and proliferative activity of cells was observed, as well as pronounced changes in the structure of the hydrogel. The described scaffold cryostorage protocol could become the basis for the development of storage protocols for such tissue engineering products, and for helping to extend the possibilities of their clinical use while accelerating their commercialization.

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