Theory and Application of Pulse Amplitude Modulated Chlorophyll Fluorometry in Coral Health Assessment

2015 ◽  
pp. 506-523 ◽  
Author(s):  
Peter J. Ralph ◽  
Ross Hill ◽  
Martina A. Doblin ◽  
Simon K. Davy
Botany ◽  
2018 ◽  
Vol 96 (11) ◽  
pp. 805-811 ◽  
Author(s):  
Paula Tummon Flynn ◽  
David Garbary ◽  
Irene Novaczek ◽  
Anthony Miller ◽  
Pedro A. Quijón

Irish moss (Chondrus crispus Stackhouse) is a red alga that is common in Atlantic Canada. A unique strain of this species, the giant Irish moss, grows in a single location and is under strict protection. Unlike the common coastal form, the giant Irish moss reproduces solely by fragmentation and is found in gametophyte form. A 99.9% decline in giant Irish moss abundance (1999–2012) prompted this study to address two questions: whether the giant Irish moss remains 100% vegetative and gametophytic, and whether such decline is related to factors leading to a reduction in photosynthetic health. Six populations of the common Irish moss strain were compared with two populations of giant Irish moss, and their life history phases determined using a resorcinol method. The common Irish moss populations exhibited a 65%–86% ratio of gametophytic:tetrasporophytic fronds, while both giant strain populations were 100% gametophytic. Photosynthetic efficiency was measured with Pulse-Amplitude-Modulation (PAM) fluorometry and neither giant moss population had significantly different quantum yield values from the littoral populations. Hence, these analyses provided no evidence of giant Irish moss being exposed to particular sources of stress linked to water or habitat quality and alternative factors explaining the decline of this unique strain are proposed.


2017 ◽  
Vol 3 (4) ◽  
pp. 14
Author(s):  
Akbar Reza ◽  
Retno Peni Sancayaningsih

<p class="Els-Abstract-text">Despite its status as District-Based Marine Protected Area, studies pertaining coral reef biodiversity never been conducted in Olele that located in the Gulf of Tomini, biodiversity hotspot in the Coral Triangle. Hence, we aims to investigate diversity, distribution and abundance of scleractinian coral, underpinning the health of reef ecosystems. This study was conducted using Line Intercept Transect on three research stations which were divided into three main zonations, reef flat (3 m depth), upper reef slope (10 m depth) and reef slope (18 m to 20 m depth). Fifty meters long transect were laid in each zonation, thus total transect were nine. The results showed that there were 35 species of scleractinian coral from 12 families. Each zonation has a different pattern of abundance and dominant life form. Based on coral health assessment using Coral Mortality Index (CMI) and the ratio between living and dead coral coverage, coral reef ecosystems in Olele were in healthy condition with the range value 0.29 ± 0.1 and 3.60 ± 1.14. This preliminary study should be used as a basic reference for database and novel direction of conservation management strategy in District-Based Marine Protected Area Olele.</p><p><strong>Keywords: </strong>district-based marine protected area; olele; lit; sclecartinia;life form; zonation.</p>


2017 ◽  
Vol 48 (6) ◽  
pp. 453-460 ◽  
Author(s):  
Michelle A. Silva ◽  
Manuel Paris ◽  
Luis M. Añez

Author(s):  
Yu. E. Moskalenko ◽  
T. I. Kravchenko ◽  
Yu. V. Novozhilova

Introduction. Slow fl uctuations in the volume and pressure of liquids in the cranial cavity have been known for a long time and have been studied for more than 100 years. However, their quantitative indicators and their practical signifi cance remain unclear until now due to the diffi culties of research. Nevertheless, it was found that they were connected with the brain activity, which made it possible to use them as one of the physiological indicators in studying the problems of manned space fl ights. Goal of research — to study the possibility of using spectral analysis of slow fl uctuations of the volume of liquids inside the cranium in order to realize the quantitative assessment of their indicators with the use of modern microelectronics and computer technology.Materials and methods. In order to solve this problem we created a complex, in which rheoencephalograph-RG-01 («Mizar») was used as a converter-modulator of physiological signals into electrical oscillations. The device was connected with the ADC (Firm «ADIstrument»), Its software allows to calculate the spectrogram with a sampling rate of 128 kHz. Studies were conducted on volunteers of younger, middle and older age groups. The respiratory rate and the electrocardiography were registered together with the rheoencephalography. Electrodes were fi xed on the volonteers′ fronto-mastoid area.Results. Slow fl uctuations the cranium representan independent physiological phenomenon. The most considerable and valuable were fl uctuations in 0,1–0,3 Hz. It was found that current frequency of 100 or 200 kHz and frequency for quantization of 80–100 kHz was optimal for performing their spectrograms. The structure of such diagram consists of 4–7 peaks with amplitude of 0,4–0,7 units compared with REG pulse amplitude. They depend on age and are characterized by hemispheric asymmetry. Spectral diagrams of slow fl ucation inside cranium are representing inpendent physiological phenomenon. These fl uctuations are not connected by common origin, with heart activity and respiration. They are connected by nature with brain activity and PRM.Conclusion. Can be an informative method for diagnostic and assessment of general status of osteopathic patients well as for the assessment of mechanisms of action of some osteopathic techniques.


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