Use of Chromogenic In Situ Hybridization to Identify MYCN Gene Copy Number in Neuroblastoma Using Routine Tissue Sections

2006 ◽  
Vol 30 (5) ◽  
pp. 635-642 ◽  
Author(s):  
Paul S. Thorner ◽  
Michael Ho ◽  
Susan Chilton-MacNeill ◽  
Maria Zielenska
Keyword(s):  
In Situ Hybridization ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Tissue Sections ◽  
Mycn Gene ◽  
Chromogenic In Situ Hybridization

scholarly journals In situ analysis of FGFR2 mRNA and comparison with FGFR2 gene copy number by dual-color in situ hybridization in a large cohort of gastric cancer patients

2017 ◽  
Vol 21 (3) ◽  
pp. 401-412 ◽  
Author(s):  
Yasutoshi Kuboki ◽  
Christoph A. Schatz ◽  
Karl Koechert ◽  
Sabine Schubert ◽  
Janine Feng ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
In Situ Hybridization ◽  
Cancer Patients ◽  
Copy Number ◽  
Gene Copy Number ◽  
In Situ Analysis ◽  
Gene Copy ◽  
Fgfr2 Gene ◽  
Gastric Cancer Patients

Gene copy number of epidermal growth factor receptor (EGFR) by fluorescent in situ hybridization (FISH) in head and neck squamous cell carcinomas (HNSCC) is closely correlated with EGFR protein levels assessed by automated quantitative protein analysis (AQUA)

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 6023-6023
Author(s):  
P. Weinberger ◽  
A. Psyrri ◽  
P. Kountourakis ◽  
T. Rampias ◽  
C. Sasaki ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Protein Expression ◽  
Protein Content ◽  
Gene Amplification ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Egfr Gene ◽  
Protein Levels

6023 Background: EGFR overexpression correlates with recurrence and with treatment resistance in HNSCC. The mechanisms of EGFR protein overexpression are poorly understood. Nonetheless, previous investigators have not demonstrated a correlation between EGFR gene copy number and protein content, using conventional immunohistochemistry (IHC). The aim of this study was to evaluate the relationship of EGFR gene copy number and protein expression utilizing fluorescence in situ hybridization (FISH) and AQUA, a novel, immunohistochemical method of automated quantitative in situ proteomic analysis which permits subcellular localization. Methods: A tissue microarray composed of 137 HNSCC treated with (chemo)radiation was constructed and analyzed for EGFR copy number by FISH (Vysis/Abbot) and EGFR protein expression (DAKO antibody) using AQUA analysis of EGFR staining scored on a scale of 0–255 and by conventional IHC. Agreement was assessed using kappa. Results: Sixteen (15%) of one-hundred six tumors with FISH results demonstrated EGFR high polysomy and/or gene amplification (FISH+). AQUA demonstrated a range of 3.6–102.2; protein levels assessed by AQUA in the FISH amplified cases were significantly higher (p =0.008) than in the FISH non- amplified ones. Using the EGFR 75th percentile as a cut-off, AQUA and FISH showed significant agreement (percentage of overall agreement 82%, kappa=0.458, p=0.003). To the contrary there was no concordance between FISH and conventional IHC results in this series. Conclusions: The discrepancy between EGFR gene amplification rate and protein expression by IHC reported previously may be due to the limitations and nonquantitative nature of conventional IHC. EGFR protein content correlates with gene copy number if protein content is quantitated and automatically analyzed, as with AQUA. No significant financial relationships to disclose.

scholarly journals TERT and AURKA Gene Copy Number Gains Enhance the Detection of Acral Lentiginous Melanomas by Fluorescence in Situ Hybridization

2014 ◽  
Vol 16 (2) ◽  
pp. 198-206 ◽  
Author(s):  
Alba Diaz ◽  
Joan Anton Puig-Butillé ◽  
Alexandra Valera ◽  
Concha Muñoz ◽  
Dolors Costa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy

scholarly journals Correlation between MET Gene Copy Number by Silver In Situ Hybridization and Protein Expression by Immunohistochemistry in Non-small Cell Lung Cancer

2012 ◽  
Vol 7 (2) ◽  
pp. 340-347 ◽  
Author(s):  
Rafal Dziadziuszko ◽  
Murry W. Wynes ◽  
Shalini Singh ◽  
Bernadette Reyna Asuncion ◽  
James Ranger-Moore ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Protein Expression ◽  
Small Cell Lung Cancer ◽  
Copy Number ◽  
Gene Copy Number ◽  
Small Cell ◽  
Gene Copy ◽  
Small Cell Lung

HER2 Amplification and Overexpression Is Not Present in Pediatric Osteosarcoma: A Tissue Microarray Study

2005 ◽  
Vol 8 (5) ◽  
pp. 525-532 ◽  
Author(s):  
Gino R. Somers ◽  
Michael Ho ◽  
Maria Zielenska ◽  
Jeremy A. Squire ◽  
Paul S. Thorner
Keyword(s):  
In Situ Hybridization ◽  
Gene Copy Number ◽  
Prognostic Indicator ◽  
Gene Copy ◽  
Tyrosine Kinase Receptor ◽  
Her2 Expression ◽  
Her2 Gene ◽  
Her2 Protein ◽  
Chromogenic In Situ Hybridization

The HER2 gene, located on 17q, encodes a 185-kD transmembrane tyrosine kinase receptor. Amplification of this gene with overexpression of the gene product occurs in about 30% of cases of breast cancer and is considered to be a poor prognostic indicator for this tumor. Results for HER2 expression in osteosarcoma are controversial, with some studies reporting up to 61% of positive cases and others reporting only negative results. Further, expression of HER2 is reported to be a favorable prognostic indicator by some groups and unfavorable by others. The present study used tissue microarrays containing 34 samples of osteosarcoma from 18 patients to analyze HER2 expression by immunohistochemistry and gene copy number by chromogenic in situ hybridization. The microarray included 13 pretreatment biopsies, 11 posttreatment resection specimens, and 10 resected metastases and comprised 18 osteoblastic, 6 chondroblastic, 5 fibroblastic, and 5 mixed subtypes. HER2 protein expression was seen in 4 of 34 (12%) tumor samples that originated from 2 of 18 patients (11%). The staining pattern was consistently weak and focal, and immunohistochemical overexpression of the HER2 protein, defined as complete membrane positivity, was never observed. Further, the presence of HER2 gene amplification was not detected in any osteosarcoma by chromogenic in situ hybridization. Therefore, therapies based on antibodies directed against the HER2 protein are unlikely to have much value in the treatment of pediatric osteosarcomas. From a technical standpoint, this study also demonstrates the value of tissue micro-arrays in screening tumors at the protein and gene levels using conventional light microscopy.

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