Promoting Long‐Term Cultivation of Motor Neurons for 3D Neuromuscular Junction Formation of 3D In Vitro Using Central‐Nervous‐Tissue‐Derived Bioink

2021 ◽  
pp. 2100581
Author(s):  
Jeong Sik Kong ◽  
Xuan Huang ◽  
Yeong‐Jin Choi ◽  
Hee‐Gyeong Yi ◽  
Junsu Kang ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Motor Neurons ◽  
Nervous Tissue ◽  
Central Nervous Tissue ◽  
Junction Formation

scholarly journals Electrical stimulation accelerates neuromuscular junction formation through ADAM19/neuregulin/ErbB signaling in vitro

2013 ◽  
Vol 545 ◽  
pp. 29-34 ◽  
Author(s):  
Takahiro Fukazawa ◽  
Masaya Matsumoto ◽  
Takeshi Imura ◽  
Elham Khalesi ◽  
Teruyuki Kajiume ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Neuromuscular Junction ◽  
Junction Formation ◽  
Erbb Signaling

scholarly journals Long-term in vitro maintenance of neuromuscular junction activity of Drosophila larvae

2003 ◽  
Vol 134 (2) ◽  
pp. 247-255 ◽  
Author(s):  
Ryan Ball ◽  
Bin Xing ◽  
Philip Bonner ◽  
Joseph Shearer ◽  
Robin L Cooper
Keyword(s):  
Neuromuscular Junction ◽  
Drosophila Larvae ◽  
In Vitro Maintenance

Long-Term Expression of Two Interacting Motor Pattern-Generating Networks in the Stomatogastric System of Freely Behaving Lobster

1998 ◽  
Vol 79 (3) ◽  
pp. 1396-1408 ◽  
Author(s):  
Stefan Clemens ◽  
Denis Combes ◽  
Pierre Meyrand ◽  
John Simmers
Keyword(s):  
Motor Neurons ◽  
Motor Pattern ◽  
Burst Duration ◽  
Cycle Period ◽  
Gastric Mill ◽  
Pyloric Network ◽  
Freely Behaving ◽  
Stomatogastric System

Clemens, Stefan, Denis Combes, Pierre Meyrand, and John Simmers. Long-term expression of two interacting motor pattern-generating networks in the stomatogastric system of freely behaving lobster. J. Neurophysiol. 79: 1396–1408, 1998. Rhythmic movements of the gastric mill and pyloric regions of the crustacean foregut are controlled by two stomatogastric neuronal networks that have been intensively studied in vitro. By using electromyographic recordings from the European lobster, Homarus gammarus, we have monitored simultaneously the motor activity of pyloric and gastric mill muscles for ≤3 mo in intact and freely behaving animals. Both pyloric and gastric mill networks are almost continuously active in vivo regardless of the presence of food. In unfed resting animals kept under “natural-like” conditions, the pyloric network expresses the typical triphasic pattern seen in vitro but at considerably slower cycle periods (2.5–3.5 s instead of 1–1.5 s). Gastric mill activity occurs at mean cycle periods of 20–50 s compared with 5–10 s in vitro but may suddenly stop for up to tens of minutes, then restart without any apparent behavioral reason. When conjointly active, the two networks express a strict coupling that involves certain but not all motor neurons of the pyloric network. The posterior pyloric constrictor muscles, innervated by a total of 8 pyloric (PY) motor neurons, are influenced by the onset of each gastric mill medial gastric/lateral gastric(MG/LG) neuron powerstroke burst, and for one cycle, PY neuron bursts may attain >300% of their mean duration. However, the duration of activity in the lateral pyloric constrictor muscle, innervated by the unique lateral pyloric (LP) motor neuron, remains unaffected by this perturbation. During this period after gastric perturbation, LP neuron and PY neurons thus express opposite burst-to-period relationships in that LP neuron burst duration is independent of the ongoing cycle period, whereas PY neuron burst duration changes with period length. In vitro the same type of gastro-pyloric interaction is observed, indicating that it is not dependent on sensory inputs. Moreover, this interaction is intrinsic to the stomatogastric ganglion itself because the relationship between the two networks persists after suppression of descending inputs to the ganglion. Intracellular recordings reveal that thisgastro-pyloric interaction originates from the gastric MG and LG neurons of the gastric network, which inhibit the pyloric pacemaker ensemble. As a consequence, the pyloric PY neurons, which are inhibited by the pyloric dilator (PD) neurons of the pyloric pacemaker group, extend their activity during the time that PD neuron is held silent. Moreover, there is evidence for a pyloro-gastric interaction, apparently rectifying, from the pyloric pacemakers back to the gastric MG/LG neuron group.

PROTEIN SYNTHESIS IN CENTRAL NERVOUS TISSUE: STUDIES ON RETINA IN VITRO

1976 ◽  
Vol 27 (5) ◽  
pp. 987-997 ◽  
Author(s):  
J. M. Parks ◽  
A. Ames ◽  
F. B. Nesbett
Keyword(s):  
Protein Synthesis ◽  
Nervous Tissue ◽  
Central Nervous Tissue

MEASUREMENT OF FUNCTION IN AN IN VITRO PREPARATION OF MAMMALIAN CENTRAL NERVOUS TISSUE

1960 ◽  
Vol 23 (6) ◽  
pp. 676-691 ◽  
Author(s):  
Adelbert Ames ◽  
Bennett S. Gurian
Keyword(s):  
Nervous Tissue ◽  
Central Nervous Tissue

Electrospun nanofibers facilitate better alignment, differentiation, and long-term culture in an in vitro model of the neuromuscular junction (NMJ)

2018 ◽  
Vol 6 (12) ◽  
pp. 3262-3272 ◽  
Author(s):  
Baiwen Luo ◽  
Lingling Tian ◽  
Nuan Chen ◽  
Seeram Ramakrishna ◽  
Nitish Thakor ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Culture System ◽  
In Vitro Model ◽  
Electrospun Nanofibers ◽  
Nanofibrous Scaffold ◽  
Long Term Culture ◽  
Vitro Model

An electrospun nanofibrous scaffold is used as a novel in vitro culture system to provide long-term support for NMJ formation.

TRANSPORT OF LEUCINE AND SODIUM IN CENTRAL NERVOUS TISSUE: STUDIES ON RETINA IN VITRO

1976 ◽  
Vol 27 (5) ◽  
pp. 999-1015 ◽  
Author(s):  
A. Ames ◽  
J. M. Parks ◽  
F. B. Nesbett
Keyword(s):  
Nervous Tissue ◽  
Central Nervous Tissue

scholarly journals ARIA is concentrated in the synaptic basal lamina of the developing chick neuromuscular junction.

1995 ◽  
Vol 130 (6) ◽  
pp. 1423-1434 ◽  
Author(s):  
A D Goodearl ◽  
A G Yee ◽  
A W Sandrock ◽  
G Corfas ◽  
G D Fischbach
Keyword(s):  
Neuromuscular Junction ◽  
Basal Lamina ◽  
Motor Neurons ◽  
Neuromuscular Junctions ◽  
Primary Cultures ◽  
Cholinergic Neurons ◽  
Synaptic Cleft ◽  
Motor Axons ◽  
Amino Terminal

ARIA is a member of a family of polypeptide growth and differentiation factors that also includes glial growth factor (GGF), neu differentiation factor, and heregulin. ARIA mRNA is expressed in all cholinergic neurons of the central nervous systems of rats and chicks, including spinal cord motor neurons. In vitro, ARIA elevates the rate of acetylcholine receptor incorporation into the plasma membrane of primary cultures of chick myotubes. To study whether ARIA may regulate the synthesis of junctional synaptic acetylcholine receptors in chick embryos, we have developed riboprobes and polyclonal antibody reagents that recognize isoforms of ARIA that include an amino-terminal immunoglobulin C2 domain and examined the expression and distribution of ARIA in motor neurons and at the neuromuscular junction. We detected significant ARIA mRNA expression in motor neurons as early as embryonic day 5, around the time that motor axons are making initial synaptic contacts with their target muscle cells. In older embryos and postnatal animals, we found ARIA protein concentrated in the synaptic cleft at neuromuscular junctions, consistent with transport down motor axons and release at nerve terminals. At high resolution using immunoelectron microscopy, we detected ARIA immunoreactivity exclusively in the synaptic basal lamina in a pattern consistent with binding to synapse specific components on the presynaptic side of the basal lamina. These results support a role for ARIA as a trophic factor released by motor neuron terminals that may regulate the formation of mature neuromuscular synapses.

A Method for Dissection and Electrical Study In Vitro of Mammalian Central Nervous Tissue

Science ◽  
1951 ◽  
Vol 114 (2960) ◽  
pp. 300-302 ◽  
Author(s):  
D. O. Rudin ◽  
G. Eisenman
Keyword(s):  
Nervous Tissue ◽  
Central Nervous Tissue ◽  
Electrical Study
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