Rhizoma Paridis of Paris polyphylla var. y unnanensis genotype classification and fluorescence visual identification using SNPs of ITS sequence

2021 ◽  
Author(s):  
Hongxiang Yin ◽  
Kaiyuan Zhang ◽  
Zixuan Ren ◽  
Jiawen Zhao ◽  
Chi Gao ◽  
...  
Keyword(s):  
Its Sequence ◽  
Visual Identification ◽  
Paris Polyphylla

scholarly journals Genotype classification and fluorescence visual identification of the Rhizoma Paridis of Paris polyphylla var. yunnanensis based on the SNPs of ITS sequence 

2020 ◽  
Author(s):  
Chi Gao ◽  
Kaiyuan Zhang ◽  
Qinghe Wang ◽  
Ling Zhao ◽  
Rong Chen ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
False Negative ◽  
Identification System ◽  
Accurate Identification ◽  
Negative Results ◽  
Visual Identification ◽  
False Negative Results ◽  
Paris Polyphylla ◽  
Green Fluorescent ◽  
First Time

Abstract A fluorescent visual identification system of Paris polyphylla var. yunnanensis was established based on internal transcribed spacer barcoding. It is proposed for the first time that P. polyphylla var. yunnanensis should be divided into two types of genotypes: YN-I and YN-II according to single nucleotide polymorphism of internal transcribed spacer. In order to avoid false-negative results, two pairs of specific primers for YN-I and YN-II were designed, respectively, and specific visual fluorescent identification systems was established by SYBR Green I fluorescent dye was directly introduced into the PCR system which can be observed directly with the naked eye for the green fluorescent color of PCR system. Therefrom, it has realized the rapid and directly visual identification of two genotypes of P. polyphylla var. yunnanensis from its common nine adulterants. This study proposed for the first time the existence of different genotypes on the legal basis of Rhizoma Paridis, and provided a model for the accurate identification of different genotypes.

Genotype Classification and Fluorescence Visual Identification of the Rhizoma Paridis of Paris Polyphylla Var. Yunnanensis based on the SNPs of ITS Sequence

2020 ◽  
Author(s):  
Chi Gao ◽  
Kaiyuan Zhang ◽  
Qinghe Wang ◽  
Ling Zhao ◽  
Rong Chen ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
False Negative ◽  
Identification System ◽  
Accurate Identification ◽  
Negative Results ◽  
Visual Identification ◽  
False Negative Results ◽  
Paris Polyphylla ◽  
Green Fluorescent ◽  
First Time

Abstract A fluorescent visual identification system of Paris polyphylla var. yunnanensis was established based on internal transcribed spacer barcoding. It is proposed for the first time that P. polyphylla var. yunnanensis should be divided into two types of genotypes: YN-I and YN-II according to single nucleotide polymorphism of internal transcribed spacer. In order to avoid false-negative results, two pairs of specific primers for YN-I and YN-II were designed, respectively, and specific visual fluorescent identification systems was established by SYBR Green I fluorescent dye was directly introduced into the PCR system which can be observed directly with the naked eye for the green fluorescent color of PCR system. Therefrom, it has realized the rapid and directly visual identification of two genotypes of P. polyphylla var. yunnanensis from its common nine adulterants. This study proposed for the first time the existence of different genotypes on the legal basis of Rhizoma Paridis, and provided a model for the accurate identification of different genotypes.

Genetic diversity of Peucedanum japonicum using Internal Transcribed Spacer (ITS) sequence

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
YH Kim ◽  
JA Ryuk ◽  
BS Ko ◽  
JW Lee ◽  
SE Oh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Internal Transcribed Spacer ◽  
Its Sequence

Visual Identification of Gilson Lapis

1982 ◽  
Vol 18 (2) ◽  
pp. 114-118
Author(s):  
R. Keith Mitchell
Keyword(s):  
Visual Identification

Phylogenetic assessment of five filamentous bacteria isolated from bulking activated sludges

1998 ◽  
Vol 37 (4-5) ◽  
pp. 303-306 ◽  
Author(s):  
R. Howarth ◽  
I. M. Head ◽  
R. F. Unz
Keyword(s):  
Ribosomal Rna ◽  
Oligonucleotide Probe ◽  
Strong Relationship ◽  
Bootstrap Support ◽  
Its Sequence ◽  
Target Sequence ◽  
Common Ancestry ◽  
Filamentous Bacteria ◽  
Axenic Strains ◽  
Rrna Sequences

Nearly complete 16S ribosomal RNA (rRNA) sequences were determined for fully characterised axenic strains of Thiothrix, Eikelboom type O21N, and Eikelboom type 1701 originally isolated from bulking activated sludges. Thiothrix strains formed a monophyletic group (100% bootstrap support) with previously described Thiothrix nivea strain JP2 and Thiothrix ramosa. Eikelboom type O21N strain AP3 revealed a sufficiently strong relationship to the Thiothrix group to suggest a common ancestry for the two organism although it was not possible to designate type 021N as a species of Thiothrix. Eikelboom type 1701 contained within its sequence the target sequence of an oligonucleotide probe for the detection of Sphaerotilus natans.

scholarly journals Constraints on the ecomorphological convergence of zooplanktivorous butterflyfishes

2021 ◽  
Author(s):  
Jennifer R Hodge ◽  
Yutong Song ◽  
Molly A Wightman ◽  
Analisa Milkey ◽  
Binh Tran ◽  
...  
Keyword(s):  
Evolutionary History ◽  
Swimming Performance ◽  
Morphological Evolution ◽  
Distinct Type ◽  
Ancestral State ◽  
Multiple Traits ◽  
Visual Identification ◽  
Form Function ◽  
Morphological Distinction ◽  
Foraging Modes

Abstract Whether distantly related organisms evolve similar strategies to meet the demands of a shared ecological niche depends on their evolutionary history and the nature of form-function relationships. In fishes, the visual identification and consumption of microscopic zooplankters, selective zooplanktivory, is a distinct type of foraging often associated with a suite of morphological specialisations. Previous work has identified inconsistencies in the trajectory and magnitude of morphological change following transitions to selective zooplanktivory, alluding to the diversity and importance of ancestral effects. Here we investigate whether transitions to selective zooplanktivory have influenced the morphological evolution of marine butterflyfishes (family Chaetodontidae), a group of small-prey specialists well known for several types of high-precision benthivory. Using Bayesian ancestral state estimation, we inferred the recent evolution of zooplanktivory among benthivorous ancestors that hunted small invertebrates and browsed by picking or scraping coral polyps. Traits related to the capture of prey appear to be functionally versatile with little morphological distinction between species with benthivorous and planktivorous foraging modes. In contrast, multiple traits related to prey detection or swimming performance are evolving toward novel, zooplanktivore-specific optima. Despite a relatively short evolutionary history, general morphological indistinctiveness, and evidence of constraint on the evolution of body size, convergent evolution has closed a near significant amount of the morphological distance between zooplanktivorous species. Overall, our findings describe the extent to which the functional demands associated with selective zooplanktivory have led to generalisable morphological features among butterflyfishes and highlight the importance of ancestral effects in shaping patterns of morphological convergence.

scholarly journals Visual identification of individual Holstein-Friesian cattle via deep metric learning

2021 ◽  
Vol 185 ◽  
pp. 106133
Author(s):  
William Andrew ◽  
Jing Gao ◽  
Siobhan Mullan ◽  
Neill Campbell ◽  
Andrew W. Dowsey ◽  
...  
Keyword(s):  
Metric Learning ◽  
Visual Identification ◽  
Holstein Friesian ◽  
Deep Metric Learning ◽  
Friesian Cattle

scholarly journals Identifying Heated Rocks Through Feldspar Luminescence Analysis (pIRIR290) and a Critical Evaluation of Macroscopic Assessment

2021 ◽  
Vol 4 (2) ◽  
Author(s):  
E. Pop ◽  
F. H. Reidsma ◽  
T. Reimann ◽  
M. J. Sier ◽  
C. E. S. Arps ◽  
...  
Keyword(s):  
Quantitative Method ◽  
Critical Evaluation ◽  
Middle Palaeolithic ◽  
Proof Of Concept ◽  
Last Interglacial ◽  
Luminescence Analysis ◽  
Thermal Alteration ◽  
Concept Study ◽  
Visual Identification ◽  
Visual Assessments

AbstractThroughout (pre)history, non-flint rocks have been used to structure fireplaces, to retain heat, to boil liquids, and to cook food. Thus far, the identification of heated non-flint rocks in archaeological contexts largely depends on a visual (macroscopic) assessment using criteria thought to be diagnostic for thermal alteration. However, visual identification can be subject to observer bias, and some heat-induced traces can be quite difficult to distinguish from other types of weathering or discolouration. In this paper, we present feldspar luminescence analysis as an independent, objective way to identify heated non-flint rocks and to evaluate the results against the established visual macroscopic method for the identification of such pieces. This is done by submitting manuported rocks with and without inferred macroscopic characteristics of heating, originating from the Last Interglacial, Middle Palaeolithic site Neumark-Nord 2/2 (Germany), to feldspar luminescence analysis (pIRIR290). Results of the feldspar luminescence analysis are compared with the visual assessments. This proof of concept study demonstrates the potential of luminescence analyses as an independent, quantitative method for the identification of heated rocks—and their prehistoric applications like hot-stone cooking, specifically for cases where macroscopic assessment cannot provide reliable determinations.

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