Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions

2012 ◽  
Vol 124 (29) ◽  
pp. 7297-7301 ◽  
Author(s):  
Anwei Zhu ◽  
Qiang Qu ◽  
Xiangling Shao ◽  
Biao Kong ◽  
Yang Tian
Keyword(s):  
In Vivo Imaging ◽  
Copper Ions ◽  
Fluorescent Sensor ◽  
Dual Emission ◽  
Carbon Dot ◽  
Cellular Copper

Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions

2012 ◽  
Vol 51 (29) ◽  
pp. 7185-7189 ◽  
Author(s):  
Anwei Zhu ◽  
Qiang Qu ◽  
Xiangling Shao ◽  
Biao Kong ◽  
Yang Tian
Keyword(s):  
In Vivo Imaging ◽  
Copper Ions ◽  
Fluorescent Sensor ◽  
Dual Emission ◽  
Carbon Dot ◽  
Cellular Copper

A carbon-dot-based dual-emission probe for ultrasensitive visual detection of copper ions

2018 ◽  
Vol 42 (24) ◽  
pp. 19771-19778 ◽  
Author(s):  
Yunsuo Kuang ◽  
Linfeng Chen ◽  
Jiahui Lu ◽  
Xike Tian ◽  
Chao Yang ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Carbon Dots ◽  
Visual Detection ◽  
Copper Ions ◽  
Fluorescent Sensor ◽  
Dual Emission ◽  
Carbon Dot

In this work, we successfully developed an ultrasensitive dual-emission fluorescent sensor (CRB) for instant visual determination of Cu2+ by modifying a Cu-sensitive rhodamine B derivative (RB) onto photostable carboxyl modified carbon dots (CDs).

scholarly journals Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Nami Kitajima ◽  
Kenji Takikawa ◽  
Hiroshi Sekiya ◽  
Kaname Satoh ◽  
Daisuke Asanuma ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Sensor ◽  
Atp Release ◽  
Extracellular Atp ◽  
Cysteine Residue ◽  
Spatiotemporal Resolution ◽  
Extracellular Signaling ◽  
Ratiometric Measurement ◽  
Atp Binding Protein

Adenosine 5’ triphosphate (ATP) is a ubiquitous extracellular signaling messenger. Here, we describe a method for in-vivo imaging of extracellular ATP with high spatiotemporal resolution. We prepared a comprehensive set of cysteine-substitution mutants of ATP-binding protein, Bacillus FoF1-ATP synthase ε subunit, labeled with small-molecule fluorophores at the introduced cysteine residue. Screening revealed that the Cy3-labeled glutamine-105 mutant (Q105C-Cy3; designated ATPOS) shows a large fluorescence change in the presence of ATP, with submicromolar affinity, pH-independence, and high selectivity for ATP over ATP metabolites and other nucleotides. To enable in-vivo validation, we introduced BoNT/C-Hc for binding to neuronal plasma membrane and Alexa Fluor 488 for ratiometric measurement. The resulting ATPOS complex binds to neurons in cerebral cortex of living mice, and clearly visualized a concentrically propagating wave of extracellular ATP release in response to electrical stimulation. ATPOS should be useful to probe the extracellular ATP dynamics of diverse biological processes in vivo.

scholarly journals A genetically encoded fluorescent sensor for in vivo imaging of GABA

2019 ◽  
Vol 16 (8) ◽  
pp. 763-770 ◽  
Author(s):  
Jonathan S. Marvin ◽  
Yoshiteru Shimoda ◽  
Vincent Magloire ◽  
Marco Leite ◽  
Takashi Kawashima ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Sensor

In vitro and in vivo imaging application of a 1,8-naphthalimide-derived Zn2+ fluorescent sensor with nuclear envelope penetrability

2013 ◽  
Vol 49 (97) ◽  
pp. 11430 ◽  
Author(s):  
Changli Zhang ◽  
Zhipeng Liu ◽  
Yunling Li ◽  
Weijiang He ◽  
Xiang Gao ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
In Vivo Imaging ◽  
Fluorescent Sensor ◽  
Imaging Application

1,8-Naphthalimide-based colorimetric and fluorescent sensor for recognition of GMP, TMP, and UMP and its application in in vivo imaging

2014 ◽  
Vol 55 (44) ◽  
pp. 6131-6136 ◽  
Author(s):  
Li Mei Zhang ◽  
Lin E. Guo ◽  
Xue Mei Li ◽  
Yong Gang Shi ◽  
Gao Fen Wu ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Sensor

In vivo imaging of hepatocellular nitric oxide using a hepatocyte-targeting fluorescent sensor

2018 ◽  
Vol 54 (52) ◽  
pp. 7231-7234 ◽  
Author(s):  
Peisheng Zhang ◽  
Yong Tian ◽  
Hui Liu ◽  
Junyu Ren ◽  
Hong Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
In Vivo Imaging ◽  
Water Solubility ◽  
Fluorescent Sensor ◽  
High Sensitivity ◽  
No Sensor ◽  
Excellent Selectivity

A hepatocyte-targeting fluorescent NO sensor has been fabricated with good water solubility, excellent selectivity, and high sensitivity (∼1.62 nM).

scholarly journals Author response: Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

2020 ◽  
Author(s):  
Nami Kitajima ◽  
Kenji Takikawa ◽  
Hiroshi Sekiya ◽  
Kaname Satoh ◽  
Daisuke Asanuma ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Fluorescent Sensor ◽  
Extracellular Atp ◽  
Author Response ◽  
Hybrid Type ◽  
The Brain
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