Spatial Accuracy of Predictive Saccades Determines the Performance of Continuous Visuomotor Action

In a table tennis rally, players perform interceptive actions on a moving ball continuously in a short time, such that the acquisition process of visual information is an important determinant of the performance of the action. However, because it is technically hard to measure gaze movement in a real game, little is known about how gaze behavior is conducted during the continuous visuomotor actions and contributes to the performance. To examine these points, we constructed a novel psychophysical experiment model enabling a continuous visuomotor task without spatial movement of any body parts, including the arm and head, and recorded the movement of the gaze and effector simultaneously at high spatiotemporal resolution. In the task, Gabor patches (target) moved one after another at a constant speed from right to left at random vertical positions on an LC display. Participants hit the target with a cursor moving vertically on the left side of the display by controlling their prehensile force on a force sensor. Participants hit the target with the cursor using a rapid-approaching movement (rapid cursor approach, RCA). Their gaze also showed rapid saccadic approaching movement (saccadic eye approach, SEA), reaching the predicted arrival point of the target earlier than the cursor. The RCA reached in or near the Hit zone in the successful (Hit) trial, but ended up away from it in the unsuccessful (Miss) trial, suggesting the spatial accuracy of the RCA determines the task's success. The SEA in the Hit trial ended nearer the target than the Miss trial. The spatial accuracy of the RCA diminished when the target disappeared 100 ms just after the end of the SEA, suggesting that visual information acquired after the saccade acted as feedback information to correct the cursor movement online for the cursor to reach the target. There was a target speed condition that the target disappearance did not compromise RCA's spatial accuracy, implying the possible RCA correction based on the post-saccadic gaze location information. These experiments clarified that gaze behavior conducted during fast continuous visuomotor actions enables online correction of the ongoing interceptive movement of an effector, improving visuomotor performance.

3D nanomechanical mapping of subcellular and sub-nuclear structures of living cells by multi-harmonic AFM with long-tip microcantilevers

Recent developments such as multi-harmonic Atomic Force Microscopy (AFM) techniques have enabled fast, quantitative mapping of nanomechanical properties of living cells. Due to their high spatiotemporal resolution, these methods provide new insights into changes of mechanical properties of subcellular structures due to disease or drug response. Here, we propose three new improvements to significantly improve the resolution, identification, and mechanical property quantification of sub-cellular and sub-nuclear structures using multi-harmonic AFM on living cells. First, microcantilever tips are streamlined using long-carbon tips to minimize long-range hydrodynamic interactions with the cell surface, to enhance the spatial resolution of nanomechanical maps and minimize hydrodynamic artifacts. Second, simultaneous Spinning Disk Confocal Microscopy (SDC) with live-cell fluorescent markers enables the unambiguous correlation between observed heterogeneities in nanomechanical maps with subcellular structures. Third, computational approaches are then used to estimate the mechanical properties of sub-nuclear structures. Results are demonstrated on living NIH 3T3 fibroblasts and breast cancer MDA-MB-231 cells, where properties of nucleoli, a deep intracellular structure, were assessed. The integrated approach opens the door to study the mechanobiology of sub-cellular structures during disease or drug response.

Structure Analysis of the Sea Breeze Based on Doppler Lidar and Its Impact on Pollutants

The Doppler lidar system can accurately obtain wind profiles with high spatiotemporal resolution, which plays an increasingly important role in the research of atmospheric boundary layers and sea–land breeze. In September 2019, Doppler lidars were used to carry out observation experiments of the atmospheric wind field and pollutants in Shenzhen. Weather Research and Forecasting showed that the topography of Hongkong affected the sea breeze to produce the circumfluence flow at low altitudes. Two sea breezes from the Pearl River Estuary and the northeast of Hong Kong arrived at the observation site in succession, changing the wind direction from northeast to southeast. Based on the wind profiles, the structural and turbulent characteristics of the sea breeze were analyzed. The sea breeze front was accurately captured by the algorithm based on fuzzy logic, and its arrival time was 17:30 on 25 September. The boundary between the sea breeze and the return flow was separated by the edge enhancement algorithm. From this, the height of the sea breeze head (about 1100 m) and the thickness of the sea breeze layer (about 700 m) can be obtained. The fluctuated height of the boundary and the spiral airflow nearby revealed the Kelvin–Helmholtz instability. The influence of the Kelvin–Helmholtz instability could be delivered to the near-surface, which was verified by the spatiotemporal change of the horizontal wind speed and momentum flux. The intensity of the turbulence under the control of the sea breeze was significantly lower than that under the land breeze. The turbulent intensity was almost 0.1, and the dissipation rate was between 10−4 and 10−2 m2·s−3 under the land breeze. The turbulent intensity was below 0.05, and the dissipation rate was between 10−5 and 10−3 m2·s−3 under the sea breeze. The turbulent parameters showed peaks and large gradients at the boundary and the sea breeze front. The peak value of the turbulent intensity was around 0.3, and the dissipation rate was around 0.1 m2·s−3. The round-trip effect of sea–land breeze caused circulate pollutants. The recirculation factor was maintained at 0.5–0.6 at heights where the sea and land breeze alternately controlled (below 600 m), as well as increasing with a decreasing duration of the sea breeze. The factor exceeded 0.9 under the control of the high-altitude breeze (above 750 m). The convergence and rise of the airflow at the front led to collect pollutants, causing a sharp decrease in air quality when the sea breeze front passed.

Longitudinal River Monitoring and Modelling Substantiate the Impact of Weirs on Nitrogen Dynamics

The fluvial nitrogen dynamics at locations around weirs are still rarely studied in detail. Eulerian data, often used by conventional river monitoring and modelling approaches, lags the spatial resolution for an unambiguous representation. With the aim to address this knowledge gap, the present study applies a coupled 1D hydrodynamic–water quality model to a 26.9 km stretch of an upland river. Tailored simulations were performed for river sections with water retention and free-flow conditions to quantify the weirs’ influences on nitrogen dynamics. The water quality data were sampled with Eulerian and Lagrangian strategies. Despite the limitations in terms of required spatial discretization and simulation time, refined model calibrations with high spatiotemporal resolution corroborated the high ammonification rates (0.015 d−1) on river sections without weirs and high nitrification rates (0.17 d−1 ammonium to nitrate, 0.78 d−1 nitrate to nitrite) on river sections with weirs. Additionally, using estimations of denitrification based on typical values for riverbed sediment as a reference, we could demonstrate that in our case study, weirs can improve denitrification substantially. The produced backwater lengths can induce a means of additional nitrogen removal of 0.2-ton d−1 (10.9%) during warm and low-flow periods.

Retrograde Analysis of Calcium Signaling by CaMPARI2 Shows Cytosolic Calcium in Chondrocytes Is Unaffected by Parabolic Flights

Calcium (Ca2+) elevation is an essential secondary messenger in many cellular processes, including disease progression and adaptation to external stimuli, e.g., gravitational load. Therefore, mapping and quantifying Ca2+ signaling with a high spatiotemporal resolution is a key challenge. However, particularly on microgravity platforms, experiment time is limited, allowing only a small number of replicates. Furthermore, experiment hardware is exposed to changes in gravity levels, causing experimental artifacts unless appropriately controlled. We introduce a new experimental setup based on the fluorescent Ca2+ reporter CaMPARI2, onboard LED arrays, and subsequent microscopic analysis on the ground. This setup allows for higher throughput and accuracy due to its retrograde nature. The excellent performance of CaMPARI2 was demonstrated with human chondrocytes during the 75th ESA parabolic flight campaign. CaMPARI2 revealed a strong Ca2+ response triggered by histamine but was not affected by the alternating gravitational load of a parabolic flight.

Fixed and Mobile Low-Cost Sensing Approaches for Microclimate Monitoring in Urban Areas: A Preliminary Study in the City of Bolzano (Italy)

In the current scenario of massive urbanization and global climate change, an intelligent monitoring of the environmental variables is becoming fundamental to ensure good living conditions in cities. Indeed, the acquisition of data with high spatiotemporal resolution can enable the assessment of environmental vulnerabilities in urban areas towards the definition of responsive adaptation and mitigation strategies. In this context, the current work presents a two-fold approach based on low-cost cloud-connected sensors for (i) fixed and (ii) mobile monitoring of several environmental parameters. This paper, which focuses on the measurement aspects of the urban micro-climate, describes in detail the hardware and software components of both approaches, and how to exploit them for setting up a field campaign. The methods were tested in the city of Bolzano (Italy), demonstrating their suitability for identifying the spatial variability of the microclimate in relation to the urban morphology, and for highlighting the presence of the urban heat island and estimating its intensity.

Canopy Solar-Induced Chlorophyll Fluorescence and Its Link to Transpiration in a Temperate Evergreen Needleleaf Forest during the Fall Transition

Northern hemisphere evergreen needleleaf forest (ENF) contributes a significant fraction of global water exchange but regional transpiration (T) observation in ENF ecosystems is still challenging. Traditional remote sensing techniques and terrestrial biosphere models reproduce the transpiration seasonality with difficulty, and with large uncertainties. Solar-induced chlorophyll fluorescence (SIF) emission from vegetation correlates to photosynthesis at multiple spatial and temporal scales. However, how SIF links to transpiration of evergreen forest during seasonal transition is unclear. Here, we explored the relationship between canopy SIF and T retrieved from ground observation towers in ENF. We also examined the role of meteorological and soil factors on the relationship between SIF and T. A slow decrease of SIF and T with a fast reduction in photosynthetically active radiation (PAR), air temperature, vapor pressure deficit (VPD), soil temperature and soil water content (SWC) were found in the ENF during the fall transition. The correlation between SIF and T at hourly and daily scales varied significantly among different months (Pearson correlation coefficient = 0.29–0.68, p < 0.01). SIF and T were significantly linearly correlated at hourly (R2 = 0.53, p < 0.001) and daily (R2 = 0.67, p < 0.001) timescales in the October. Air temperature and PAR were the major moderating factors for the relationship between SIF and T in the fall transition. Soil water content (SWC) influenced the SIF-T relationship at an hourly scale. Soil temperature and VPD’s effect on the SIF-T relationship was evident at a daily scale. This study can help extend the possibility of constraining ecosystem T by SIF at an unprecedented spatiotemporal resolution during season transitions.

Receptor-Ribosome Coupling: A Link Between Extrinsic Signals and mRNA Translation in Neuronal Compartments

Axons receive extracellular signals that help to guide growth and synapse formation during development and to maintain neuronal function and survival during maturity. These signals relay information via cell surface receptors that can initiate local intracellular signaling at the site of binding, including local messenger RNA (mRNA) translation. Direct coupling of translational machinery to receptors provides an attractive way to activate this local mRNA translation and change the local proteome with high spatiotemporal resolution. Here, we first discuss the increasing evidence that different external stimuli trigger translation of specific subsets of mRNAs in axons via receptors and thus play a prominent role in various processes in both developing and mature neurons. We then discuss the receptor-mediated molecular mechanisms that regulate local mRNA translational with a focus on direct receptor-ribosome coupling. We advance the idea that receptor-ribosome coupling provides several advantages over other translational regulation mechanisms and is a common mechanism in cell communication. Expected final online publication date for the Annual Review of Neuroscience, Volume 45 is July 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Red Light Optogenetics in Neuroscience

Optogenetics, a field concentrating on controlling cellular functions by means of light-activated proteins, has shown tremendous potential in neuroscience. It possesses superior spatiotemporal resolution compared to the surgical, electrical, and pharmacological methods traditionally used in studying brain function. A multitude of optogenetic tools for neuroscience have been created that, for example, enable the control of action potential generation via light-activated ion channels. Other optogenetic proteins have been used in the brain, for example, to control long-term potentiation or to ablate specific subtypes of neurons. In in vivo applications, however, the majority of optogenetic tools are operated with blue, green, or yellow light, which all have limited penetration in biological tissues compared to red light and especially infrared light. This difference is significant, especially considering the size of the rodent brain, a major research model in neuroscience. Our review will focus on the utilization of red light-operated optogenetic tools in neuroscience. We first outline the advantages of red light for in vivo studies. Then we provide a brief overview of the red light-activated optogenetic proteins and systems with a focus on new developments in the field. Finally, we will highlight different tools and applications, which further facilitate the use of red light optogenetics in neuroscience.