scholarly journals Author response: Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

2020 ◽  
Author(s):  
Nami Kitajima ◽  
Kenji Takikawa ◽  
Hiroshi Sekiya ◽  
Kaname Satoh ◽  
Daisuke Asanuma ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Fluorescent Sensor ◽  
Extracellular Atp ◽  
Author Response ◽  
Hybrid Type ◽  
The Brain

scholarly journals Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Nami Kitajima ◽  
Kenji Takikawa ◽  
Hiroshi Sekiya ◽  
Kaname Satoh ◽  
Daisuke Asanuma ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Sensor ◽  
Atp Release ◽  
Extracellular Atp ◽  
Cysteine Residue ◽  
Spatiotemporal Resolution ◽  
Extracellular Signaling ◽  
Ratiometric Measurement ◽  
Atp Binding Protein

Adenosine 5’ triphosphate (ATP) is a ubiquitous extracellular signaling messenger. Here, we describe a method for in-vivo imaging of extracellular ATP with high spatiotemporal resolution. We prepared a comprehensive set of cysteine-substitution mutants of ATP-binding protein, Bacillus FoF1-ATP synthase ε subunit, labeled with small-molecule fluorophores at the introduced cysteine residue. Screening revealed that the Cy3-labeled glutamine-105 mutant (Q105C-Cy3; designated ATPOS) shows a large fluorescence change in the presence of ATP, with submicromolar affinity, pH-independence, and high selectivity for ATP over ATP metabolites and other nucleotides. To enable in-vivo validation, we introduced BoNT/C-Hc for binding to neuronal plasma membrane and Alexa Fluor 488 for ratiometric measurement. The resulting ATPOS complex binds to neurons in cerebral cortex of living mice, and clearly visualized a concentrically propagating wave of extracellular ATP release in response to electrical stimulation. ATPOS should be useful to probe the extracellular ATP dynamics of diverse biological processes in vivo.

scholarly journals A fluorescent sensor for the real-time measurement of extracellular oxytocin dynamics in the brain

2021 ◽  
Author(s):  
Daisuke Ino ◽  
Hiroshi Hibino ◽  
Masaaki Nishiyama
Keyword(s):  
Real Time ◽  
Dynamic Range ◽  
Fluorescent Sensor ◽  
Viral Gene ◽  
Affinity Ligand ◽  
Fluorescence Measurements ◽  
Animal Behaviors ◽  
Green Fluorescent ◽  
The Brain

Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe MTRIAOT, a G protein-coupled receptor-based green fluorescent OT sensor with a large dynamic range, optimal affinity, ligand specificity to OT orthologs, minimal effects on downstream signaling, and long-term fluorescence stability. By combining viral gene delivery and fiber photometry-mediated fluorescence measurements, we demonstrated the utility of MTRIAOT for real-time detection of brain OT dynamics in living mice. Importantly, MTRIAOT-mediated measurements revealed "OT oscillation," a hitherto unknown rhythmic change in OT levels in the brain. MTRIAOT will allow the analysis of OT dynamics in a wide variety of physiological and pathological processes.

Development of Radioiodinated Benzofuran Derivatives for in Vivo Imaging of Prion Deposits in the Brain

2019 ◽  
Vol 5 (12) ◽  
pp. 2003-2013 ◽  
Author(s):  
Takeshi Fuchigami ◽  
Masao Kawasaki ◽  
Ryusuke Koyama ◽  
Mari Nakaie ◽  
Takehiro Nakagaki ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
The Brain

In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation

APOPTOSIS ◽  
2010 ◽  
Vol 16 (2) ◽  
pp. 198-207 ◽  
Author(s):  
Matteo Scabini ◽  
Fabio Stellari ◽  
Paolo Cappella ◽  
Sara Rizzitano ◽  
Gemma Texido ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Caspase 3 ◽  
Early Stage

Novel quinoxaline derivatives for in vivo imaging of β-amyloid plaques in the brain

2011 ◽  
Vol 21 (14) ◽  
pp. 4193-4196 ◽  
Author(s):  
Mengchao Cui ◽  
Masahiro Ono ◽  
Hiroyuki Kimura ◽  
Boli Liu ◽  
Hideo Saji
Keyword(s):  
In Vivo Imaging ◽  
Amyloid Plaques ◽  
Quinoxaline Derivatives ◽  
Β Amyloid ◽  
The Brain

2021 Frank Stinchfield Award: A novel cemented hip hemiarthroplasty infection model with real-time in vivo imaging in rats

2021 ◽  
Vol 103-B (7 Supple B) ◽  
pp. 9-16
Author(s):  
William J. Hadden ◽  
Mazen Ibrahim ◽  
Mariam Taha ◽  
Kerstin Ure ◽  
Yun Liu ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Biofilm Formation ◽  
Joint Infection ◽  
Volumetric Analysis ◽  
In Vivo Model ◽  
Infection Model ◽  
Sprague Dawley ◽  
Hip Hemiarthroplasty

Aims The aims of this study were to develop an in vivo model of periprosthetic joint infection (PJI) in cemented hip hemiarthroplasty, and to monitor infection and biofilm formation in real-time. Methods Sprague-Dawley rats underwent cemented hip hemiarthroplasty via the posterior approach with pre- and postoperative gait assessments. Infection with Staphylococcus aureus Xen36 was monitored with in vivo photoluminescent imaging in real-time. Pre- and postoperative gait analyses were performed and compared. Postmortem micro (m) CT was used to assess implant integration; field emission scanning electron microscopy (FE-SEM) was used to assess biofilm formation on prosthetic surfaces. Results All animals tolerated surgery well, with preservation of gait mechanics and weightbearing in control individuals. Postoperative in vivo imaging demonstrated predictable evolution of infection with logarithmic signal decay coinciding with abscess formation. Postmortem mCT qualitative volumetric analysis showed high contact area and both cement-bone and cement-implant interdigitation. FE-SEM revealed biofilm formation on the prosthetic head. Conclusion This study demonstrates the utility of a new, high-fidelity model of in vivo PJI using cemented hip hemiarthroplasty in rats. Inoculation with bioluminescent bacteria allows for non-invasive, real-time monitoring of infection. Cite this article: Bone Joint J 2021;103-B(7 Supple B):9–16.

Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice

2006 ◽  
Vol 1 (1) ◽  
pp. 476-485 ◽  
Author(s):  
Blandine Franke-Fayard ◽  
Andrew P Waters ◽  
Chris J Janse
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Malaria Parasites ◽  
Rodent Malaria
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