In vivo imaging of hepatocellular nitric oxide using a hepatocyte-targeting fluorescent sensor

2018 ◽  
Vol 54 (52) ◽  
pp. 7231-7234 ◽  
Author(s):  
Peisheng Zhang ◽  
Yong Tian ◽  
Hui Liu ◽  
Junyu Ren ◽  
Hong Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
In Vivo Imaging ◽  
Water Solubility ◽  
Fluorescent Sensor ◽  
High Sensitivity ◽  
No Sensor ◽  
Excellent Selectivity

A hepatocyte-targeting fluorescent NO sensor has been fabricated with good water solubility, excellent selectivity, and high sensitivity (∼1.62 nM).

Mitochondria-targeted colorimetric and fluorescent probes for hypochlorite and their applications for in vivo imaging

2014 ◽  
Vol 50 (63) ◽  
pp. 8640-8643 ◽  
Author(s):  
Ji-Ting Hou ◽  
Ming-Yu Wu ◽  
Kun Li ◽  
Jin Yang ◽  
Kang-Kang Yu ◽  
...  
Keyword(s):  
Response Time ◽  
Fluorescent Probes ◽  
In Vivo Imaging ◽  
High Sensitivity ◽  
Rapid Response ◽  
Excellent Selectivity

Two probes that exhibited high sensitivity and excellent selectivity toward ClO−among ROS with a rapid response time were presented, which could selectively detect ClO−in the mitochondria of the cells.

High-sensitivity in vivo imaging for tumors using a spectral up-conversion nanoparticle NaYF4: Yb3+, Er3+ in cooperation with a microtubulin inhibitor

Nanoscale ◽  
2012 ◽  
Vol 4 (13) ◽  
pp. 3901 ◽  
Author(s):  
Yanchun Wei ◽  
Qun Chen ◽  
Baoyan Wu ◽  
Aiguo Zhou ◽  
Da Xing
Keyword(s):  
In Vivo Imaging ◽  
High Sensitivity

scholarly journals Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Nami Kitajima ◽  
Kenji Takikawa ◽  
Hiroshi Sekiya ◽  
Kaname Satoh ◽  
Daisuke Asanuma ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Sensor ◽  
Atp Release ◽  
Extracellular Atp ◽  
Cysteine Residue ◽  
Spatiotemporal Resolution ◽  
Extracellular Signaling ◽  
Ratiometric Measurement ◽  
Atp Binding Protein

Adenosine 5’ triphosphate (ATP) is a ubiquitous extracellular signaling messenger. Here, we describe a method for in-vivo imaging of extracellular ATP with high spatiotemporal resolution. We prepared a comprehensive set of cysteine-substitution mutants of ATP-binding protein, Bacillus FoF1-ATP synthase ε subunit, labeled with small-molecule fluorophores at the introduced cysteine residue. Screening revealed that the Cy3-labeled glutamine-105 mutant (Q105C-Cy3; designated ATPOS) shows a large fluorescence change in the presence of ATP, with submicromolar affinity, pH-independence, and high selectivity for ATP over ATP metabolites and other nucleotides. To enable in-vivo validation, we introduced BoNT/C-Hc for binding to neuronal plasma membrane and Alexa Fluor 488 for ratiometric measurement. The resulting ATPOS complex binds to neurons in cerebral cortex of living mice, and clearly visualized a concentrically propagating wave of extracellular ATP release in response to electrical stimulation. ATPOS should be useful to probe the extracellular ATP dynamics of diverse biological processes in vivo.

C12. Performances and applicability of electrochemical NO-sensor for in vivo detection of nitric oxide in tumor-bearing mice

Nitric Oxide ◽  
2007 ◽  
Vol 17 ◽  
pp. 22-23
Author(s):  
S. Griveau ◽  
C. Dumézy ◽  
J. Seguin ◽  
G.G. Chabot ◽  
D. Scherman ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Tumor Bearing ◽  
In Vivo Detection ◽  
No Sensor

scholarly journals A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4768 ◽  
Author(s):  
Zongcheng Wang ◽  
Huihuang Zheng ◽  
Chengliang Zhang ◽  
Dongfang Tang ◽  
Qiyao Wu ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
High Sensitivity ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Quantitative Detection ◽  
Fast Reaction ◽  
Detection Range ◽  
Long Wavelength ◽  
Turn On

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.

Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions

2012 ◽  
Vol 51 (29) ◽  
pp. 7185-7189 ◽  
Author(s):  
Anwei Zhu ◽  
Qiang Qu ◽  
Xiangling Shao ◽  
Biao Kong ◽  
Yang Tian
Keyword(s):  
In Vivo Imaging ◽  
Copper Ions ◽  
Fluorescent Sensor ◽  
Dual Emission ◽  
Carbon Dot ◽  
Cellular Copper

scholarly journals A genetically encoded fluorescent sensor for in vivo imaging of GABA

2019 ◽  
Vol 16 (8) ◽  
pp. 763-770 ◽  
Author(s):  
Jonathan S. Marvin ◽  
Yoshiteru Shimoda ◽  
Vincent Magloire ◽  
Marco Leite ◽  
Takashi Kawashima ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Sensor

Activatable photoacoustic and fluorescent probe of nitric oxide for cellular and in vivo imaging

2018 ◽  
Vol 267 ◽  
pp. 403-411 ◽  
Author(s):  
Shichao Wang ◽  
Zhi Li ◽  
Yubin Liu ◽  
Gang Feng ◽  
Jun Zheng ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
In Vivo Imaging
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