Anti-Ribosomal P Protein Autoantibodies From Patients With Neuropsychiatric Lupus Impair Memory in Mice

Objective Anti-ribosomal P antibodies (anti-P) are strongly associated with neuropsychiatric lupus. This study was designed to determine whether these antibodies are capable of causing electro-oscillogram (EOSG) and behavior alterations in rats. Methods IgG fraction anti-P positive and affinity-purified anti-P antibodies were injected intraventricularly in rats. Sequential cortical and subcortical EOSGs were analyzed during 30 days. IgG anti-Ro/SS-A and normal IgG were used as controls. Results All 13 animals injected with IgG anti-P demonstrated a high prevalence of polyspikes, diffusely distributed in hippocampal fields and cerebral cortex. These abnormalities persisted approximately a month. Remarkably, an identical electrical disturbance was observed with the inoculation of affinity-purified anti-P antibodies. The EOSG alterations were associated with behavioral disorders with varying degrees of severity in every animal injected with anti-P. In contrast, no changes in EOSG or behavioral disturbances were observed in the control group. Conclusion Our study indicates that anti-P antibodies can directly induce electrophysiological dysfunction in central nervous system particularly in hippocampus and cortex associated with behavior disturbances.

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Anti-ribosomal P protein antibody: an autoreactivity devoid of prognostic value in patients with autoimmune hepatitis

Liver International ◽

10.1111/liv.12585 ◽

2014 ◽

Vol 34 (9) ◽

pp. 1446-1446 ◽

Cited By ~ 3

Author(s):

Luigi Muratori ◽

Chiara Masi ◽

Paolo Muratori

Keyword(s):

Autoimmune Hepatitis ◽

Prognostic Value ◽

P Protein ◽

Ribosomal P

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Lupus Eritematosus Sistemik pada Pria

Majalah Kedokteran Andalas ◽

10.25077/mka.v43.i2.p159-168.2020 ◽

2020 ◽

Vol 43 (2) ◽

pp. 159

Author(s):

Zikry Aulia Hidayat

Keyword(s):

Lupus Erythematosus ◽

Vte Prophylaxis ◽

Report Result ◽

Oral Ulcers ◽

Methyl Prednisolone ◽

P Protein ◽

Laboratory Results ◽

Male Patients ◽

Ribosomal P ◽

Complex Autoimmune Disease

Objective: Systemic lupus erythematosus (SLE) is a complex autoimmune disease involving many systems. Highest incidence and prevalence of SLE is found in Northern America 23.2/100,000 population/year and 241/100,000 population. According to sex differences, SLE is predominantly occurs in women than men with ratio 15:1 to 22:1. This discrepancy often causes delay in diagnosing SLE in male patients. Method: Case report. Result: We reported a male patient aged 21 years with pain of his joints, hyperpigmentation lesions on his face, alopesia, oral ulcers and decrease of body weight. Laboratory results showed increases in AST, ALT and D-Dimer, and from ANA Profile examination we got several positives results such as RNP/Sm (RNP/Sm) (++), Sm (Sm) (+), Ro-52 recombinant (52) (+), PCNA (PCNA) (+), DsDNA (DNA) (+), Nucleosome (NUC) (+), Histone (HI) (++), Ribosomal-P-protein (RIB) (+++) dan AMA-M2 (M2) (+). This patient met SLE criteria based on ACR 1997, SLICC 2012 and EULAR/ACR 2018. Patient was given oral methyl prednisolone 16-16-8 mg and VTE prophylaxis with subcutaneous heparin 2x5000 IU. Conclusion: SLE occurs rarely in male patients than female patients and has more diverse manifestations.

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Structural and Functional Investigation and Pharmacological Mechanism of Trichosanthin, a Type 1 Ribosome-Inactivating Protein

Toxins ◽

10.3390/toxins10080335 ◽

2018 ◽

Vol 10 (8) ◽

pp. 335 ◽

Cited By ~ 1

Author(s):

Wei-Wei Shi ◽

Kam-Bo Wong ◽

Pang-Chui Shaw

Keyword(s):

Ribosomal Rna ◽

Ribosome Inactivating Protein ◽

Pharmacological Activities ◽

Pharmacological Mechanism ◽

Functional Studies ◽

P Protein ◽

Ribosomal Stalk ◽

Functional Investigation ◽

Ribosomal P

Trichosanthin (TCS) is an RNA N-glycosidase that depurinates adenine-4324 in the conserved α-sarcin/ricin loop (α-SRL) of rat 28 S ribosomal RNA (rRNA). TCS has only one chain, and is classified as type 1 ribosome-inactivating protein (RIP). Our structural studies revealed that TCS consists of two domains, with five conserved catalytic residues Tyr70, Tyr111, Glu160, Arg163 and Phe192 at the active cleft formed between them. We also found that the structural requirements of TCS to interact with the ribosomal stalk protein P2 C-terminal tail. The structural analyses suggest TCS attacks ribosomes by first binding to the C-terminal domain of ribosomal P protein. TCS exhibits a broad spectrum of biological and pharmacological activities including anti-tumor, anti-virus, and immune regulatory activities. This review summarizes an updated knowledge in the structural and functional studies and the mechanism of its multiple pharmacological effects.

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