Comparison of immobilized and free enzyme systems in industrial production of short‐chain fructooligosaccharides from sucrose using a techno‐economic approach

2019 ◽  
Vol 13 (5) ◽  
pp. 1274-1288 ◽  
Author(s):  
Oscar K. K. Bedzo ◽  
Mohsen Mandegari ◽  
Johann F. Görgens
Keyword(s):  
Industrial Production ◽  
Free Enzyme ◽  
Short Chain ◽  
Economic Approach ◽  
Enzyme Systems

Chemical profiling of edible seaweed (Ochrophyta) extracts and assessment of their in vitro effects on cell-free enzyme systems and on the viability of glutamate-injured SH-SY5Y cells

2018 ◽  
Vol 116 ◽  
pp. 196-206 ◽  
Author(s):  
Fátima Fernandes ◽  
Mariana Barbosa ◽  
David M. Pereira ◽  
Isabel Sousa-Pinto ◽  
Patrícia Valentão ◽  
...  
Keyword(s):  
Free Enzyme ◽  
Edible Seaweed ◽  
Chemical Profiling ◽  
Enzyme Systems ◽  
In Vitro Effects

scholarly journals Do Rumen Bacteroidetes Utilize an Alternative Mechanism for Cellulose Degradation?

mBio ◽  
2014 ◽  
Vol 5 (4) ◽  
Author(s):  
A. E. Naas ◽  
A. K. Mackenzie ◽  
J. Mravec ◽  
J. Schückel ◽  
W. G. T. Willats ◽  
...  
Keyword(s):  
Cellulose Degradation ◽  
Preliminary Evidence ◽  
Free Enzyme ◽  
Lignocellulose Degradation ◽  
Alternative Mechanism ◽  
Natural Ecosystems ◽  
Enzyme Systems ◽  
Polysaccharide Utilization Locus

ABSTRACT Uncultured and therefore uncharacterized Bacteroidetes lineages are ubiquitous in many natural ecosystems which specialize in lignocellulose degradation. However, their metabolic contribution remains mysterious, as well-studied cultured Bacteroidetes have been shown to degrade only soluble polysaccharides within the human distal gut and herbivore rumen. We have interrogated a reconstructed genome from an uncultured Bacteroidetes phylotype that dominates a switchgrass-associated community within the cow rumen. Importantly, this characterization effort has revealed the first preliminary evidence for polysaccharide utilization locus (PUL)-catalyzed conversion of cellulose. Based on these findings, we propose a further expansion of the PUL paradigm and the saccharolytic capacity of rumen Bacteroidetes species to include cellulose, the most abundant terrestrial polysaccharide on Earth. Moreover, the perspective of a cellulolytic PUL lays the foundation for PULs to be considered an alternative mechanism for cellulose degradation, next to cellulosomes and free-enzyme systems.

Chitosanase Immobilization Using Composite Carrier of Sodium Alginate/Cellulose

2011 ◽  
Vol 236-238 ◽  
pp. 2371-2377 ◽  
Author(s):  
Yan Wang ◽  
Chen Lu Zhang ◽  
Ping Li ◽  
Pei Gen Zhou ◽  
Xiao Rong Pan
Keyword(s):  
Thermal Stability ◽  
Sodium Alginate ◽  
Industrial Production ◽  
Free Enzyme ◽  
Optimum Ratio ◽  
Single Carrier ◽  
Km Value ◽  
Composite Carrier

Sodium alginate and cellulose were combined to use as a composite carrier forPseudomonassp. CUY8 chitosanase immobilization. For free enzyme, immobilized chitosanase within different carriers of sodium alginate and composite carrier, Km values were 1.919, 9.27, and 5.91µM, respectively. The increase of Km value of immobilized chitosanase with composite carrier was lower than that of single carrier. This indicates that the composite carrier of sodium alginate/ cellulose improves the affinity of chitosanase to the substrate. Furthermore, chitosanase immobilization using composite carrier shows improved thermal stability ranging from 65 to 80°C, and enzyme residual activities were more than 75%. The effects of ratio of enzyme to substrate on chitooligosaccharides (COS) production were determined, and COS yields with composite carrier was 68% at optimum ratio of 1:1. Since the immobilization process using composite carrier is simple and effective, this method could be used for the industrial production of COS.

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