Spectrofluorometric determination of clonazepam in dosage forms: Application to content uniformity testing and human plasma

Luminescence ◽  
2017 ◽  
Vol 32 (7) ◽  
pp. 1299-1306 ◽  
Author(s):  
Fathalla Belal ◽  
Fawzia Ibrahim ◽  
Zainab Sheribah ◽  
Heba Alaa
Keyword(s):  
Human Plasma ◽  
Dosage Forms ◽  
Content Uniformity ◽  
Spectrofluorometric Determination ◽  
Content Uniformity Testing

scholarly journals Spectrofluorometric Determination of Fluvoxamine in Dosage Forms, Spiked Plasma, and Real Human Plasma by Derivatization with Fluorescamine

2007 ◽  
Vol 90 (2) ◽  
pp. 376-383 ◽  
Author(s):  
Nahed El-Enany
Keyword(s):  
Human Plasma ◽  
Reaction Pathway ◽  
Biological Fluids ◽  
Pharmaceutical Formulations ◽  
Dosage Forms ◽  
Spectrofluorometric Determination ◽  
Spiked Plasma ◽  
Experimental Parameters ◽  
Good Agreement

Abstract A sensitive, simple, and selective spectrofluorometric method was developed for the determination of fluvoxamine (FXM) in pharmaceutical formulations and biological fluids. The method is based upon the reaction between the drug and fluorescamine in borate buffer of pH 8.0 to yield a highly fluorescent derivative that is measured at 481 nm after excitation at 383 nm. The different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized. The method was applied for the determination of the drug over the concentration range of 0.11.1 μg/mL with a detection limit of 0.01 μg/mL (2 10-8 M). The proposed method was successfully applied to the analysis of commercial tablets. The results obtained were in good agreement with those obtained using a reported spectrophotometric method. The method was applied for the determination of FXM in spiked human plasma with recovery (n = 4) of 97.32 1.23%, while that in real human plasma (n = 3) was 90.79 2.73%. A proposal for the reaction pathway is presented.

Spectrophotometric and Spectrofluorometric Determination of Heptaminol and mexiletine in Their Dosage Forms

1997 ◽  
Vol 30 (11) ◽  
pp. 2029-2043 ◽  
Author(s):  
Abdel Fattah M. El Walily ◽  
Fawzy A. El-Yazbi ◽  
Saeid F. Belal ◽  
Omayma Abdel-Razak
Keyword(s):  
Dosage Forms ◽  
Spectrofluorometric Determination
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