Spectrofluorimetric investigation with green analytical procedures for estimation of bambuterol and terbutaline: Application to pharmaceutical dosage forms and content uniformity testing

Luminescence ◽  
2018 ◽  
Vol 34 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Samah Abo El Abass Mohamed ◽  
Mohamed I. El-Awady
Keyword(s):  
Dosage Forms ◽  
Content Uniformity ◽  
Pharmaceutical Dosage Forms ◽  
Content Uniformity Testing ◽  
Analytical Procedures

scholarly journals Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test

2016 ◽  
Vol 14 (1) ◽  
pp. 258-266 ◽  
Author(s):  
Sayed M Derayea ◽  
Mahmoud A Omar ◽  
Mohamed Aboel-Kasem Abdel-Lateef ◽  
Ahmed I. Hassan
Keyword(s):  
Fluorescence Quenching ◽  
Beta Blockers ◽  
Dosage Forms ◽  
Ion Pair ◽  
Content Uniformity ◽  
Eosin Y ◽  
Pharmaceutical Dosage Forms ◽  
Quenching Effect ◽  
Spectrofluorimetric Method

AbstractA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.

Simple spectrofluorimetric methods for determination of two pharmaceutically active compounds; salmeterol and trimebutine in their raw materials and pharmaceutical dosage forms in nano concentrations and testing of content uniformity

2018 ◽  
Vol 10 (37) ◽  
pp. 4511-4517
Author(s):  
M. I. Walash ◽  
Samah Abo El Abass ◽  
M. E. Fathy
Keyword(s):  
Aqueous Solution ◽  
Raw Materials ◽  
Dosage Forms ◽  
Fluorescence Properties ◽  
Content Uniformity ◽  
Pharmaceutically Active Compounds ◽  
Native Fluorescence ◽  
Active Compounds ◽  
Pharmaceutical Dosage Forms

Simple and rapid spectrofluorimetric methods based on the native fluorescence properties of salmeterol in its aqueous solution and enhanced native fluorescence of trimebutine were developed.

scholarly journals Determination of metoprolol in pure and pharmaceutical dosage forms by spectrofluorometry and high performance liquid chromatography

2011 ◽  
Vol 17 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Bilal Yilmaz ◽  
Kadem Meral ◽  
Ali Asci ◽  
Yavuz Organer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Solvent System ◽  
Dosage Forms ◽  
Content Uniformity ◽  
Pharmaceutical Dosage Forms ◽  
Relative Standard ◽  
Limit Of Quantitation

In this study, a new and rapid spectrofluorometry and high performance liquid chromatography (HPLC) methods were developed for determination of metoprolol in pure and pharmaceutical dosage forms. The solvent system, wavelength of detection and chromatographic conditions were optimized in order to maximize the sensitivity of both the proposed methods. The linearity was established over the concentration range of 50-4000 ng ml-1 for spectrofluorometry and 5.0-300 ng ml-1 for HPLC methods. The intra- and inter-day relative standard deviation (RSD) was less than 4.14 and 3.86% for spectrofluorometry and HPLC, respectively. Limit of quantitation was determined as 30 and 5.0 ng ml-1 for spectrofluorometry and HPLC, respectively. No interference was found from tablet excipients at the selected assay conditions. The methods were applied for the quality control of commercial metoprolol dosage forms to quantify the drug and to check the formulation content uniformity.

Spectrofluorometric determination of clonazepam in dosage forms: Application to content uniformity testing and human plasma

Luminescence ◽  
2017 ◽  
Vol 32 (7) ◽  
pp. 1299-1306 ◽  
Author(s):  
Fathalla Belal ◽  
Fawzia Ibrahim ◽  
Zainab Sheribah ◽  
Heba Alaa
Keyword(s):  
Human Plasma ◽  
Dosage Forms ◽  
Content Uniformity ◽  
Spectrofluorometric Determination ◽  
Content Uniformity Testing

scholarly journals Validation of a UV Spectrophotometric Method for the Determination of Melatonine in Solid Dosage Forms

2001 ◽  
Vol 84 (5) ◽  
pp. 1352-1357 ◽  
Author(s):  
Renato F Pérez ◽  
Igor G Lemus ◽  
Rony V Bocic ◽  
Mauricio V Pérez ◽  
Rubén García-Madrid
Keyword(s):  
Accurate Method ◽  
The United States ◽  
Dosage Forms ◽  
Content Uniformity ◽  
Solid Dosage Forms ◽  
Pharmaceutical Dosage Forms ◽  
Solid Dosage ◽  
Pharmaceutical Form ◽  
Ph Range

Abstract The aim of the work described in this paper was to provide a fast, easy, inexpensive, precise, and accurate method for the determination of melatonine in solid pharmaceutical dosage forms. The developed method is based on a UV first-derivative spectrophotometric determination, which exhibits excellent linearity in aqueous solutions (r2 = 0.996) for analyte concentrations of 1.5–4.5 mg/dL within a pH range of 5–9. Neither excipients present in the formulation nor indole adulterants, such as tryptophan (up to 5%), interfere with the assay. A study of variation parameters showed that sonication temperature was the main factor for successful determination. At temperatures of <45°C, the sample dissolved completely, and accurate spectrophotometric measurements were obtained. A study was conducted of all the parameters established by the United States Pharmacopeia, 23rd Rev., to validate an analytical method for a solid pharmaceutical form, i.e., linearity, range, accuracy, precision, and specificity. All the parameters were in accordance with the acceptance criteria of the Comité de Guías Oficiales de Validación de la Dirección General de Control de Insumos para la Salud de Méjico. In addition, robustness and content uniformity tests were performed to substantiate the usefulness of the method.

Spectrophotometric Determination of Alendronate Sodium by using Sodium-1,2-Naphthoquinone-4-Sulphonate

2012 ◽  
Vol 4 (4) ◽  
pp. 1563-1568
Author(s):  
Sagar Suman Panda ◽  
Ravi Kumar B V V ◽  
D Patanaik
Keyword(s):  
Spectrophotometric Method ◽  
Absorption Maximum ◽  
Dosage Form ◽  
Dosage Forms ◽  
Alendronate Sodium ◽  
Colored Complex ◽  
Pharmaceutical Dosage Forms ◽  
Recovery Study ◽  
Assay Conditions

A simple, precise and accurate spectrophotometric method was developed for analysis of the osteoporesis drug alendronate sodium (ALS). The method is based on reaction of the drug with sodium-1,2-naphthoquinone-4-sulphonate (NQS) in presence of alkali to form a brown colored complex giving absorption maximum at 525 nm. The drug obeyed Beer’s law in the range of 5-70 µg/ml with a correlation coefficient of 0.999. The LOD and LOQ values are 1.7 µg/ml and 5.0 µg/ml, respectively. The average recoveries for recovery study were found to be in the range of 99.37%-100.46%. The R.S.D. values for intraday and inter-day precision were found to be 0.48 and 0.62, respectively. The optimized assay conditions were applied successfully for determination of ALS in pharmaceutical dosage forms. No interference was observed from the excipients present in the dosage form. The method is statistically validated as per the ICH requirements.  

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