Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. IV. Immobilization of two thermostable ?-glycosidases and optimization of a packed-bed reactor for lactose conversion

2002 ◽  
Vol 77 (6) ◽  
pp. 619-631 ◽  
Author(s):  
Inge Petzelbauer ◽  
Bernhard Kuhn ◽  
Barbara Splechtna ◽  
Klaus D. Kulbe ◽  
Bernd Nidetzky
1989 ◽  
Vol 34 (2) ◽  
pp. 242-251 ◽  
Author(s):  
G. Gonzàlez ◽  
G. Caminal ◽  
C. de Mas ◽  
J. Lòpez-Santín

2016 ◽  
Vol 12 (7) ◽  
pp. 681-689 ◽  
Author(s):  
Eng-Tong Phuah ◽  
Yee-Ying Lee ◽  
Teck-Kim Tang ◽  
Oi-Ming Lai ◽  
Thomas Shean-Yaw Choong ◽  
...  

Abstract Response surface methodology (RSM) was employed to optimize the process variables namely packed bed height (cm) and flow rates (ml/min) on diacylglycerol (DAG) production via partial hydrolysis of palm oil using immobilized Rhizomucor miehei lipase in packed bed reactor (PBR). Quadratic models were successfully developed for both DAG(y) and unhydrolyzed triacylglycerol ((un)TAG) with determination coefficient (R2) of 0.9931 and 0.9986, respectively coupled with insignificant lack of fit (p > 0.05). Optimal conditions for DAG synthesis were evaluated to be 10 cm packed bed height and 3.8 ml/min flow rate. Immobilized enzyme can be reused up to 10 times without significant changes in enzymatic activity. The partial hydrolysis under studied was found to be mass transfer-controlled.


Author(s):  
K. Nimthuphariyha ◽  
A. Usmani ◽  
N. Grisdanurak ◽  
E. Kanchanatip ◽  
M. Yan ◽  
...  

2010 ◽  
Vol 86 (1) ◽  
pp. 42-46 ◽  
Author(s):  
Reeba Panesar ◽  
Parmjit S. Panesar ◽  
Ram S. Singh ◽  
John F. Kennedy

2020 ◽  
Vol 123 ◽  
pp. 345-353
Author(s):  
T. Bavaro ◽  
I. Benucci ◽  
A. Pedrali ◽  
G. Marrubini ◽  
M. Esti ◽  
...  

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