Hydrolysis of milk/whey lactose by β galactosidase: A comparative study of stirred batch process and packed bed reactor prepared with calcium alginate entrapped enzyme

2009 ◽  
Vol 48 (1) ◽  
pp. 576-580 ◽  
Author(s):  
Toshiba Haider ◽  
Qayyum Husain
Keyword(s):  
Comparative Study ◽  
Calcium Alginate ◽  
Batch Process ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Milk Whey ◽  
Hydrolysis Of

Modeling and Optimization of Lipase-Catalyzed Partial Hydrolysis for Diacylglycerol Production in Packed Bed Reactor

2016 ◽  
Vol 12 (7) ◽  
pp. 681-689 ◽  
Author(s):  
Eng-Tong Phuah ◽  
Yee-Ying Lee ◽  
Teck-Kim Tang ◽  
Oi-Ming Lai ◽  
Thomas Shean-Yaw Choong ◽  
...  
Keyword(s):  
Immobilized Enzyme ◽  
Rhizomucor Miehei ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Partial Hydrolysis ◽  
Optimal Conditions ◽  
Lack Of Fit ◽  
Bed Height ◽  
Hydrolysis Of ◽  
Quadratic Models

Abstract Response surface methodology (RSM) was employed to optimize the process variables namely packed bed height (cm) and flow rates (ml/min) on diacylglycerol (DAG) production via partial hydrolysis of palm oil using immobilized Rhizomucor miehei lipase in packed bed reactor (PBR). Quadratic models were successfully developed for both DAG(y) and unhydrolyzed triacylglycerol ((un)TAG) with determination coefficient (R2) of 0.9931 and 0.9986, respectively coupled with insignificant lack of fit (p > 0.05). Optimal conditions for DAG synthesis were evaluated to be 10 cm packed bed height and 3.8 ml/min flow rate. Immobilized enzyme can be reused up to 10 times without significant changes in enzymatic activity. The partial hydrolysis under studied was found to be mass transfer-controlled.

scholarly journals Development of a bioreactor system for the remediation of endosulphan

2018 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
K. Jesitha ◽  
P. S. Harikumar
Keyword(s):  
Detection Limit ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Alginate Beads ◽  
Soil Samples ◽  
Packed Bed Reactor ◽  
Contaminated Water ◽  
Initial Concentration ◽  
Bioreactor System ◽  
Ca Alginate

Abstract A bioreactor system that consisted of Pseudomonas fluorescens cells immobilised in calcium-alginate beads was utilised to remediate endosulphan contaminated water and soil. A packed bed reactor system was designed for the bio-degradation of endosulphan in artificially spiked water samples (initial concentration of endosulphan: 350 µg/L). Reactor studies with cell-immobilised Ca-alginate beads were conducted after checking their efficiency through batch and column degradation studies. The results showed that the concentration of toxic isomers of endosulphan (endosulphan alpha and endosulphan beta) was below the limit in the bioreactor during the 7th day of the experiment. Experiments conducted with contaminated soil samples (initial concentration of endosulphan: 1,000 μg/kg) indicated that the toxic isomers of endosulphan degraded to below the detection limit within 10 days and monitoring of endosulphan residues on the 14th day revealed that almost complete degradation of metabolites of endosulphan had occurred. The bioreactor system designed can be scaled up for remediation of endosulphan in contaminated areas.

Hydrolysis of carbonyl sulfide over modified Al2O3 by platinum and barium in a packed-bed reactor

2019 ◽  
pp. 1-10 ◽  
Author(s):  
K. Nimthuphariyha ◽  
A. Usmani ◽  
N. Grisdanurak ◽  
E. Kanchanatip ◽  
M. Yan ◽  
...  
Keyword(s):  
Carbonyl Sulfide ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Hydrolysis Of

Hydrolysis of milk lactose in a packed bed reactor system using immobilized yeast cells

2010 ◽  
Vol 86 (1) ◽  
pp. 42-46 ◽  
Author(s):  
Reeba Panesar ◽  
Parmjit S. Panesar ◽  
Ram S. Singh ◽  
John F. Kennedy
Keyword(s):  
Packed Bed ◽  
Reactor System ◽  
Packed Bed Reactor ◽  
Yeast Cells ◽  
Immobilized Yeast ◽  
Immobilized Yeast Cells ◽  
Hydrolysis Of
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