Expanded bed adsorption as a primary recovery step for the isolation of the insulin precursor MI3 process development and scale up

2005 ◽  
Vol 93 (1) ◽  
pp. 14-20 ◽  
Author(s):  
Peter Brixius ◽  
Inger Mollerup ◽  
Ole Elvang Jensen ◽  
Markus Halfar ◽  
Jörg Thömmes ◽  
...  
Keyword(s):  
Process Development ◽  
Scale Up ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Insulin Precursor

Expanded bed adsorption at production scale: Scale-up verification, process example and sanitization of column and adsorbent

1997 ◽  
Vol 16 (2) ◽  
pp. 57 ◽  
Author(s):  
A.-K. Barnfield Frej ◽  
H. J. Johansson ◽  
S. Johansson ◽  
P. Leijon
Keyword(s):  
Scale Up ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Production Scale ◽  
Verification Process

scholarly journals Scaling-up production of plant endophytes in bioreactors: concepts, challenges and perspectives

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Seedhabadee Ganeshan ◽  
Seon Hwa Kim ◽  
Vladimir Vujanovic
Keyword(s):  
Crop Production ◽  
Process Development ◽  
Hazard Analysis ◽  
Scale Up ◽  
Process Technology ◽  
Lack Of Information ◽  
Agriculture Industry ◽  
Start Up ◽  
Plant Growth Promoting ◽  
Agriculture And Food

AbstractThe benefit of microorganisms to humans, animals, insects and plants is increasingly recognized, with intensified microbial endophytes research indicative of this realization. In the agriculture industry, the benefits are tremendous to move towards sustainable crop production and minimize or circumvent the use of chemical fertilizers and pesticides. The research leading to the identification of potential plant endophytes is long and arduous and for many researchers the challenge is ultimately in scale-up production. While many of the larger agriculture and food industries have their own scale-up and manufacturing facilities, for many in academia and start-up companies the next steps towards production have been a stumbling block due to lack of information and understanding of the processes involved in scale-up fermentation. This review provides an overview of the fermentation process from shake flask cultures to scale-up and the manufacturing steps involved such as process development optimization (PDO), process hazard analysis (PHA), pre-, in- and post-production (PIP) challenges and finally the preparation of a technology transfer package (TTP) to transition the PDO to manufacturing. The focus is on submerged liquid fermentation (SLF) and plant endophytes production by providing original examples of fungal and bacterial endophytes, plant growth promoting Penicillium sp. and Streptomyces sp. bioinoculants, respectively. We also discuss the concepts, challenges and future perspectives of the scale-up microbial endophyte process technology based on the industrial and biosafety research platform for advancing a massive production of next-generation biologicals in bioreactors.

Expanded bed adsorption as a fast technique for the large-scale purification of the complete isoform pool of Ber e 1, the major allergen from Brazil nuts

2006 ◽  
Vol 50 (3) ◽  
pp. 275-281 ◽  
Author(s):  
Evelien L. van Boxtel ◽  
Gerrit A. van Koningsveld ◽  
Stef J. Koppelman ◽  
Lambertus A. M. van den Broek ◽  
Alfons G. J. Voragen ◽  
...  
Keyword(s):  
Large Scale ◽  
Major Allergen ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Brazil Nuts

Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies

1999 ◽  
Vol 34 (2) ◽  
pp. 159-165 ◽  
Author(s):  
J. Feuser ◽  
M. Halfar ◽  
D. Lütkemeyer ◽  
N. Ameskamp ◽  
M.-R. Kula ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Culture Broth ◽  
Expanded Bed Adsorption ◽  
Expanded Bed

scholarly journals A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery

2009 ◽  
Vol 52 (2) ◽  
pp. 427-436 ◽  
Author(s):  
João Batista Severo Jr. ◽  
Roberto Rodrigues de Souza ◽  
José Carlos Curvelo Santana ◽  
Elias Basile Tambourgi
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Binding Capacity ◽  
Ion Exchange Resin ◽  
Exchange Chromatography ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Bed Height ◽  
Salt Addition ◽  
Bed Expansion

In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.

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