In situ monitoring of 3D in vitro cell aggregation using an optical imaging system

2008 ◽  
Vol 100 (1) ◽  
pp. 159-167 ◽  
Author(s):  
N.B.E. Sawyer ◽  
L.K. Worrall ◽  
J.A. Crowe ◽  
S.L. Waters ◽  
K.M. Shakesheff ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Imaging System ◽  
Cell Aggregation ◽  
In Situ Monitoring ◽  
Optical Imaging System

scholarly journals The Development and Applications of a Dual Optical Imaging System for Studying Glioma Stem Cells

2019 ◽  
Vol 18 ◽  
pp. 153601211987089 ◽  
Author(s):  
Po-An Tai ◽  
Yen-Lin Liu ◽  
Ya-Ting Wen ◽  
Chien-Min Lin ◽  
Thanh-Tuan Huynh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Brain Tumor ◽  
Optical Imaging ◽  
Fluorescent Protein ◽  
Imaging System ◽  
High Rate ◽  
Glioma Stem Cells ◽  
Optical Imaging System

Glioblastoma multiforme represents one of the deadliest brain tumor types, manifested by a high rate of recurrence and poor prognosis. The presence of glioma stem cells (GSCs) can repopulate the tumor posttreatment and resist therapeutics. A better understanding of GSC biology is essential for developing more effective interventions. We established a CD133 promoter-driven dual reporter, expressing green fluorescent protein (GFP) and firefly luciferase (CD133-LG), capable for in vitro and in vivo imaging of CD133+ GSCs. We first demonstrated the reporter enabled in vitro analyses of GSCs. DBTRG-05MG (Denver Brain Tumor Research Group 05) carrying CD133-LG (DBTRG-05MG-CD133-LG) system reported increased GFP/luciferase activities in neurospheres. Additionally, we identified and isolated CD133+/GFP+ cells with increased tumorigenic properties, stemness markers, Notch1, β-catenin, and Bruton’s tyrosine kinase (Btk). Furthermore, prolonged temozolomide (TMZ) treatment enriched GSCs (reflected by increased percentage of CD133+ cells). Subsequently, Btk inhibitor, ibrutinib, suppressed GSC generation and stemness markers. Finally, we demonstrated real-time evaluation of anti-GSC function of ibrutinib in vivo with TMZ-enriched GSCs. Tumorigenesis was noninvasively monitored by bioluminescence imaging and mice that received ibrutinib showed a significantly lower tumor burden, indicating ibrutinib as a potential GSC inhibitor. In conclusion, we established a dual optical imaging system which enables the identification of CD133+ GSCs and screening for anti-GSC drugs.

scholarly journals A comparison between Zooglider and shipboard net and acoustic mesozooplankton sensing systems

2019 ◽  
Vol 41 (4) ◽  
pp. 521-533 ◽  
Author(s):  
Benjamin M Whitmore ◽  
Catherine F Nickels ◽  
Mark D Ohman
Keyword(s):  
Optical Imaging ◽  
Imaging System ◽  
Relative Magnitude ◽  
Thin Layers ◽  
Ambient Conditions ◽  
Frequency Distributions ◽  
Sensing Systems ◽  
Vertical Scale ◽  
Optical Imaging System

Abstract Some planktonic patches have markedly higher concentrations of organisms compared to ambient conditions and are <5 m in thickness (i.e. thin layers). Conventional net sampling techniques are unable to resolve this vertical microstructure, while optical imaging systems can measure it for limited durations. Zooglider, an autonomous zooplankton-sensing glider, uses a low-power optical imaging system (Zoocam) to resolve mesozooplankton at a vertical scale of 5 cm while making concurrent physical and acoustic measurements (Zonar). In March 2017, Zooglider was compared with traditional nets (MOCNESS) and ship-based acoustics (Simrad EK80). Zoocam recorded significantly higher vertically integrated abundances of smaller copepods and appendicularians, and larger gelatinous predators and mineralized protists, but similar abundances of chaetognaths, euphausiids, and nauplii. Differences in concentrations and size-frequency distributions are attributable to net extrusion and preservation artifacts, suggesting advantages of in situ imaging of organisms by Zooglider. Zoocam detected much higher local concentrations of copepods and appendicularians (53 000 and 29 000 animals m−3, respectively) than were resolvable by nets. The EK80 and Zonar at 200 kHz agreed in relative magnitude and distribution of acoustic backscatter. The profiling capability of Zooglider allows for deeper high-frequency acoustic sampling than conventional ship-based acoustics.

scholarly journals Dual-mode optical imaging system for fluorescence image-guided surgery

2014 ◽  
Vol 39 (13) ◽  
pp. 3830 ◽  
Author(s):  
Nan Zhu ◽  
Suman Mondal ◽  
Shengkui Gao ◽  
Samuel Achilefua ◽  
Viktor Gruev ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Imaging System ◽  
Image Guided Surgery ◽  
Dual Mode ◽  
Fluorescence Image ◽  
Guided Surgery ◽  
Image Guided ◽  
Optical Imaging System

scholarly journals High speed magneto-optical imaging system to investigate motion characteristics of arc plasma in enclosed chamber

2018 ◽  
Vol 26 (18) ◽  
pp. 23156 ◽  
Author(s):  
Guogang Zhang ◽  
Zhiqiang Zhang ◽  
Youdang Xu ◽  
Jianhua Wang
Keyword(s):  
Optical Imaging ◽  
High Speed ◽  
Imaging System ◽  
Arc Plasma ◽  
Optical Imaging System ◽  
Enclosed Chamber ◽  
Motion Characteristics
Sign in / Sign up

Export Citation Format

Share Document