Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system

2008 ◽  
Vol 101 (2) ◽  
pp. 422-427 ◽  
Author(s):  
Jin-Ho Ahn ◽  
Taek-Jin Kang ◽  
Dong-Myung Kim
Keyword(s):  
Protein Synthesis ◽  
Recombinant Proteins ◽  
Mrna Stability ◽  
Expression Level ◽  
Synthesis System ◽  
Free Protein ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

A Cell-Free Protein Synthesis System from Insect Cells

2009 ◽  
pp. 31-42 ◽  
Author(s):  
Toru Ezure ◽  
Takashi Suzuki ◽  
Masamitsu Shikata ◽  
Masaaki Ito ◽  
Eiji Ando
Keyword(s):  
Protein Synthesis ◽  
Insect Cells ◽  
Synthesis System ◽  
Free Protein ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

Translational incorporation of multiple unnatural amino acids in a cell-free protein synthesis system

2014 ◽  
Vol 19 (3) ◽  
pp. 426-432 ◽  
Author(s):  
Su-Jin Oh ◽  
Kyung-Ho Lee ◽  
Ho-Cheol Kim ◽  
Christy Catherine ◽  
Hyungdon Yun ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Unnatural Amino Acids ◽  
Synthesis System ◽  
Free Protein ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

scholarly journals A cell-free protein synthesis system for high-throughput proteomics

2002 ◽  
Vol 99 (23) ◽  
pp. 14652-14657 ◽  
Author(s):  
T. Sawasaki ◽  
T. Ogasawara ◽  
R. Morishita ◽  
Y. Endo
Keyword(s):  
Protein Synthesis ◽  
High Throughput ◽  
Synthesis System ◽  
Free Protein ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

scholarly journals Cloning-Independent Expression and Analysis of ω-Transaminases by Use of a Cell-Free Protein Synthesis System

2010 ◽  
Vol 76 (18) ◽  
pp. 6295-6298 ◽  
Author(s):  
Yong-Chan Kwon ◽  
Kyung-Ho Lee ◽  
Ho-Cheol Kim ◽  
Kyuboem Han ◽  
Joo-Hyun Seo ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Function ◽  
Free Expression ◽  
Synthesis System ◽  
Free Protein ◽  
Enzyme Substrate ◽  
Information Gap ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

ABSTRACT Herewith we report the expression and screening of microbial enzymes without involving cloning procedures. Computationally predicted putative ω-transaminase (ω-TA) genes were PCR amplified from the bacterial colonies and expressed in a cell-free protein synthesis system for subsequent analysis of their enzymatic activity and substrate specificity. Through the cell-free expression analysis of the putative ω-TA genes, a number of enzyme-substrate pairs were identified in a matter of hours. We expect that the proposed strategy will provide a universal platform for bridging the information gap between nucleotide sequence and protein function to accelerate the discovery of novel enzymes.

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