scholarly journals Scalable expansion of multipotent adult progenitor cells as three-dimensional cell aggregates

2010 ◽  
Vol 108 (2) ◽  
pp. 364-375 ◽  
Author(s):  
K. Subramanian ◽  
Y. Park ◽  
C.M. Verfaillie ◽  
W.S. Hu
Keyword(s):  
Progenitor Cells ◽  
Three Dimensional ◽  
Cell Aggregates ◽  
Multipotent Adult Progenitor Cells ◽  
Dimensional Cell

Models of Cell Interaction Based on Differential Adhesion

1984 ◽  
Vol 106 (1) ◽  
pp. 36-41 ◽  
Author(s):  
S. Childress
Keyword(s):  
Three Dimensional ◽  
Numerical Algorithms ◽  
Cell Types ◽  
Cell Interaction ◽  
Cell Aggregates ◽  
Biological Cells ◽  
Mechanical Interaction ◽  
Cell Sheets ◽  
Differential Adhesion ◽  
Dimensional Cell

Recent efforts to model the mechanical interaction of aggregates of biological cells are reviewed. Differential adhesion is discussed as a means of creating a field of stress equivalent to tension elements at interfaces between unlike cell types. Several numerical algorithms are described and applied to shortening and folding of cell sheets, three-dimensional monolayers, and two-dimensional cell aggregates.

Three-dimensional cell aggregates composed of HUVECs and cbMSCs for therapeutic neovascularization in a mouse model of hindlimb ischemia

Biomaterials ◽  
2013 ◽  
Vol 34 (8) ◽  
pp. 1995-2004 ◽  
Author(s):  
Ding-Yuan Chen ◽  
Hao-Ji Wei ◽  
Kun-Ju Lin ◽  
Chieh-Cheng Huang ◽  
Chung-Chi Wang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Three Dimensional ◽  
Cell Aggregates ◽  
Hindlimb Ischemia ◽  
Dimensional Cell

scholarly journals A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement

Lab on a Chip ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 179-189 ◽  
Author(s):  
Andreas R. Thomsen ◽  
Christine Aldrian ◽  
Peter Bronsert ◽  
Yi Thomann ◽  
Norbert Nanko ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Volume Measurement ◽  
Cell Aggregates ◽  
Microwell Array ◽  
Dimensional Cell

Miniaturised conical measures for cell aggregates.

Re-Differentiation of Human Meniscus Fibrochondrocytes Differs in Three-Dimensional Cell Aggregates and Decellularized Human Meniscus Matrix Scaffolds

2019 ◽  
Vol 48 (3) ◽  
pp. 968-979
Author(s):  
Yan Liang ◽  
Alexander R. A. Szojka ◽  
Enaam Idrees ◽  
Melanie Kunze ◽  
Aillette Mulet-Sierra ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Cell Aggregates ◽  
Dimensional Cell

scholarly journals The Anti-Proliferative Effect of PI3K/mTOR and ERK Inhibition in Monolayer and Three-Dimensional Ovarian Cancer Cell Models

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 395
Author(s):  
Elizabeth Dunn ◽  
Kenny Chitcholtan ◽  
Peter Sykes ◽  
Ashley Garrill
Keyword(s):  
Ovarian Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Alternative Pathway ◽  
Three Dimensional ◽  
Cell Aggregates ◽  
Erk Inhibition ◽  
Inhibitor Combination ◽  
Dimensional Cell

Most ovarian cancer patients are diagnosed with advanced stage disease, which becomes unresponsive to chemotherapeutic treatments. The PI3K/AKT/mTOR and the RAS/RAF/MEK/ERK kinase signaling pathways are attractive targets for potential therapeutic inhibitors, due to the high frequency of mutations to PTEN, PIK3CA, KRAS and BRAF in several ovarian cancer subtypes. However, monotherapies targeting one of these pathways have shown modest effects in clinical trials. This limited efficacy of the agents could be due to upregulation and increased signaling via the adjacent alternative pathway. In this study, the efficacy of combined PI3K/mTOR (BEZ235) and ERK inhibition (SCH772984) was investigated in four human ovarian cancer cell lines, grown as monolayer and three-dimensional cell aggregates. The inhibitor combination reduced cellular proliferation in a synergistic manner in OV-90 and OVCAR8 monolayers and in OV-90, OVCAR5 and SKOV3 aggregates. Sensitivity to the inhibitors was reduced in three-dimensional cell aggregates in comparison to monolayers. OV-90 cells cultured in large spheroids were sensitive to the inhibitors and displayed a robust synergistic antiproliferative response to the inhibitor combination. In contrast, OVCAR8 spheroids were resistant to the inhibitors. These findings suggest that combined PI3K/mTOR and ERK inhibition could be a useful strategy for overcoming treatment resistance in ovarian cancer and warrants further preclinical investigation. Additionally, in some cell lines the use of different three-dimensional models can influence cell line sensitivity to PI3K/mTOR and RAS/RAF/MEK/ERK pathway inhibitors.

CYTOKINE-AUGMENTED CULTURE OF HAEMATOPOIETIC PROGENITOR CELLS IN A NOVEL THREE-DIMENSIONAL CELL GROWTH MATRIX

Cytokine ◽  
2001 ◽  
Vol 13 (6) ◽  
pp. 349-358 ◽  
Author(s):  
Naheed Banu ◽  
Michael Rosenzweig ◽  
Helen Kim ◽  
Jim Bagley ◽  
Mark Pykett
Keyword(s):  
Cell Growth ◽  
Progenitor Cells ◽  
Three Dimensional ◽  
Dimensional Cell
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