Controlling trisulfide modification in recombinant monoclonal antibody produced in fed-batch cell culture

2012 ◽  
Vol 109 (10) ◽  
pp. 2523-2532 ◽  
Author(s):  
Rashmi Kshirsagar ◽  
Kyle McElearney ◽  
Alan Gilbert ◽  
Marty Sinacore ◽  
Thomas Ryll
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Fed Batch ◽  
Recombinant Monoclonal Antibody ◽  
Batch Cell

Principle of Express Selection of Leading Producing Clones of Monoclonal Antibodies in the Development of Stable CHO-Based Cell Lines

2019 ◽  
Vol 35 (4) ◽  
pp. 65-72
Author(s):  
V.I. Pavelko ◽  
I.A. Kirik ◽  
V.N. Bade ◽  
T.O. Malygina ◽  
R.A. Khamitov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Parallel Processing ◽  
Cell Lines ◽  
Cho Cells ◽  
Fed Batch ◽  
Cho Cell ◽  
Recombinant Monoclonal Antibody ◽  
Selection Of

Growth and productive characteristics of monoclonal cell lines based on CHO cells and producing a therapeutic protein have been monitored using the robot Ambr Tap Biosystems, which permitted to identify the leading line. Twenty four clones producing a recombinant monoclonal antibody were studied under the close to industrial conditions in a fed-batch culturing mode. The ambr®15 cell culture workstation controls 24 disposable mini bioreactors, and offers parallel processing and evaluation of multiple (24) experiments in an automated bench-top system. The volumetric productivity of 24 clones determined by ELISA was 120-450 mg /L. A protocol was shown to select a leader among producing clones for further research. producing clones, mini bioreactor; Ambr Tap Biosystems, fed-batch, monoclonal antibodies, CHO cell culture

When Good Intentions Go Awry: Modification of a Recombinant Monoclonal Antibody in Chemically Defined Cell Culture by Xylosone, an Oxidative Product of Ascorbic Acid

2015 ◽  
Vol 87 (15) ◽  
pp. 7529-7534 ◽  
Author(s):  
Chris Chumsae ◽  
Patrick Hossler ◽  
Haly Raharimampionona ◽  
Yu Zhou ◽  
Sean McDermott ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Ascorbic Acid ◽  
Recombinant Monoclonal Antibody

Investigation of metabolic variability observed in extended fed batch cell culture

2013 ◽  
Vol 29 (6) ◽  
pp. 1519-1527 ◽  
Author(s):  
Alan Gilbert ◽  
Kyle McElearney ◽  
Rashmi Kshirsagar ◽  
Martin S. Sinacore ◽  
Thomas Ryll
Keyword(s):  
Cell Culture ◽  
Fed Batch ◽  
Batch Cell

scholarly journals Impact of aeration strategies on fed-batch cell culture kinetics in a single-use 24-well miniature bioreactor

2014 ◽  
Vol 82 ◽  
pp. 105-116 ◽  
Author(s):  
J.P.J. Betts ◽  
S.R.C. Warr ◽  
G.B. Finka ◽  
M. Uden ◽  
M. Town ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fed Batch ◽  
Single Use ◽  
Batch Cell
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