Physiological effects of pH gradients onEscherichia coliduring plasmid DNA production

2015 ◽  
Vol 113 (3) ◽  
pp. 598-611 ◽  
Author(s):  
José T. Cortés ◽  
Noemí Flores ◽  
Francisco Bolívar ◽  
Alvaro R. Lara ◽  
Octavio T. Ramírez
Keyword(s):  
Plasmid Dna ◽  
Physiological Effects ◽  
Ph Gradients ◽  
Dna Production ◽  
Effects Of Ph

Preliminary studies on medium and fermentation strategy design for plasmid DNA production

2007 ◽  
Vol 131 (2) ◽  
pp. S166-S167
Author(s):  
Filomena Silva ◽  
Luís Passarinha ◽  
Fani Sousa ◽  
João Queiróz ◽  
Fernanda Domingues
Keyword(s):  
Plasmid Dna ◽  
Dna Production ◽  
Fermentation Strategy ◽  
Strategy Design

Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use

2003 ◽  
Vol 79 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Carsten Voss ◽  
Torsten Schmidt ◽  
Martin Schleef ◽  
Karl Friehs ◽  
Erwin Flaschel
Keyword(s):  
Cell Density ◽  
Batch Culture ◽  
Ammonium Chloride ◽  
Plasmid Dna ◽  
High Cell Density ◽  
High Cell ◽  
Dna Production

Physiological effects of plasmid DNA transformation on Azotobacter vinelandii

1986 ◽  
Vol 32 (2) ◽  
pp. 145-148 ◽  
Author(s):  
Baernard R. Glick ◽  
Heather E. Brooks ◽  
J. J. Pasternak
Keyword(s):  
Plasmid Dna ◽  
Azotobacter Vinelandii ◽  
Genetic Manipulation ◽  
A Priori ◽  
Broad Host Range ◽  
Physiological Effects ◽  
Dna Transformation ◽  
Physiological Changes ◽  
Physiological Processes ◽  
Broad Host Range Plasmid

Genetic transformation of Azotobacter vinelandii by the introduction of broad-host-range plasmid DNA (i.e., pRK2501, RSF1010, or pGSS15) causes a number of physiological changes. As shown here, the capacity for nitrogen fixation, mean cell size, and synthesis of siderophores are decreased, whereas the production of capsular slime is enhanced. These findings suggest that the presence of plasmid DNA imposes a "metabolic load" on Azotobacter vinelandii. Therefore, it cannot be assumed a priori that the introduction of plasmid DNA into Azotobacter vinelandii will not disrupt some normal physiological processes. The implications of these findings are discussed, specifically in the context of developing Azotobacter vinelandii as an effective bacterial fertilizer by genetic manipulation.

Enhanced plasmid DNA production by enzyme-controlled glucose release and an engineered Escherichia coli

2015 ◽  
Vol 38 (4) ◽  
pp. 651-657 ◽  
Author(s):  
Elisa A. Ramírez ◽  
Daniela Velázquez ◽  
Alvaro R. Lara
Keyword(s):  
Escherichia Coli ◽  
Plasmid Dna ◽  
Glucose Release ◽  
Dna Production ◽  
Engineered Escherichia Coli

scholarly journals De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production

2012 ◽  
Vol 97 (2) ◽  
pp. 611-620 ◽  
Author(s):  
Geisa A. L. Gonçalves ◽  
Duarte M. F. Prazeres ◽  
Gabriel A. Monteiro ◽  
Kristala L. J. Prather
Keyword(s):  
Plasmid Dna ◽  
De Novo ◽  
E Coli ◽  
Dna Production

Medium design for plasmid DNA production based on stoichiometric model

2001 ◽  
Vol 36 (11) ◽  
pp. 1085-1093 ◽  
Author(s):  
Zhijun Wang ◽  
Guowei Le ◽  
Yonghui Shi ◽  
Grzegorz Węgrzyn
Keyword(s):  
Plasmid Dna ◽  
Stoichiometric Model ◽  
Dna Production ◽  
Medium Design
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