Dual-color reporter switching system to discern dimer formations of G-protein-coupled receptors using Cre/loxP site-specific recombination in yeast

2016 ◽  
Vol 113 (10) ◽  
pp. 2178-2190 ◽  
Author(s):  
Yasuyuki Nakamura ◽  
Takamichi Hashimoto ◽  
Jun Ishii ◽  
Akihiko Kondo
Keyword(s):  
G Protein ◽  
Loxp Site ◽  
G Protein Coupled Receptors ◽  
Switching System ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Dual Color ◽  
G Protein Coupled

scholarly journals Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation

2018 ◽  
Vol 217 (12) ◽  
pp. 4253-4266 ◽  
Author(s):  
Hannes Maib ◽  
Filipe Ferreira ◽  
Stéphane Vassilopoulos ◽  
Elizabeth Smythe
Keyword(s):  
G Protein ◽  
Light Chain ◽  
Transferrin Receptor ◽  
G Protein Coupled Receptors ◽  
Light Chains ◽  
Coated Pits ◽  
Selective Uptake ◽  
Site Specific ◽  
Specific Manner ◽  
G Protein Coupled

Clathrin light chains (CLCs) control selective uptake of a range of G protein–coupled receptors (GPCRs), although the mechanism by which this occurs has remained elusive thus far. In particular, site-specific phosphorylation of CLCb controls the uptake of the purinergic GPCR P2Y12, but it is dispensable for the constitutive uptake of the transferrin receptor (TfR). We demonstrate that phosphorylation of CLCb is required for the maturation of clathrin-coated pits (CCPs) through the transition of flat lattices into invaginated buds. This transition is dependent on efficient clathrin exchange regulated by CLCb phosphorylation and mediated through auxilin. Strikingly, this rearrangement is required for the uptake of P2Y12 but not TfR. These findings link auxilin-mediated clathrin exchange to early stages of CCP invagination in a cargo-specific manner. This supports a model in which CCPs invaginate with variable modes of curvature depending on the cargo they incorporate.

scholarly journals Site-Specific Fluorescent Labeling of Purified G-Protein-Coupled Receptors Using Genetically-Encoded Unnatural Amino Acids

2010 ◽  
Vol 98 (3) ◽  
pp. 291a-292a
Author(s):  
Shixin Ye ◽  
Manija A. Kazmi ◽  
Terence Duarte ◽  
Saranga Naganathan ◽  
Thomas P. Sakmar ◽  
...  
Keyword(s):  
Amino Acids ◽  
G Protein ◽  
Unnatural Amino Acids ◽  
Fluorescent Labeling ◽  
G Protein Coupled Receptors ◽  
Site Specific ◽  
G Protein Coupled

scholarly journals Dual-Color Fluorescent Labeling of G Protein-Coupled Receptors

2012 ◽  
Vol 102 (3) ◽  
pp. 518a
Author(s):  
He Tian ◽  
Saranga Naganathan ◽  
Shixin Ye ◽  
Thomas P. Sakmar ◽  
Thomas Huber
Keyword(s):  
G Protein ◽  
Fluorescent Labeling ◽  
G Protein Coupled Receptors ◽  
Dual Color ◽  
G Protein Coupled

scholarly journals Site-Specific Fluorescent Labeling of G Protein-Coupled Receptors

2011 ◽  
Vol 100 (3) ◽  
pp. 256a
Author(s):  
He Tian ◽  
Saranga Naganathan ◽  
Shixin Ye ◽  
Thomas P. Sakmar ◽  
Thomas Huber
Keyword(s):  
G Protein ◽  
Fluorescent Labeling ◽  
G Protein Coupled Receptors ◽  
Site Specific ◽  
G Protein Coupled
Sign in / Sign up

Export Citation Format

Share Document