Metabolic Engineering of Escherichia coli for Lactic Acid Production from Renewable Resources

2017 ◽  
pp. 125-145 ◽  
Author(s):  
Alfredo Martinez ◽  
María E. Rodríguez-Alegría ◽  
Maria Conceição Fernandes ◽  
Guillermo Gosset ◽  
Alejandra Vargas-Tah
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Metabolic Engineering ◽  
Renewable Resources ◽  
Acid Production ◽  
Lactic Acid Production

Inhibitory Activity of Cheese Whey Fermented with Kefir Grains

2011 ◽  
Vol 74 (1) ◽  
pp. 94-100 ◽  
Author(s):  
A. LONDERO ◽  
R. QUINTA ◽  
A. G. ABRAHAM ◽  
R. SERENO ◽  
G. DE ANTONI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Acid Production ◽  
Cheese Whey ◽  
Lactic Acid Production ◽  
Reaction Products ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Fermentation Products ◽  
E Coli ◽  
Kefir Grains

We investigated the chemical and microbiological compositions of three types of whey to be used for kefir fermentation as well as the inhibitory capacity of their subsequent fermentation products against 100 Salmonella sp. and 100 Escherichia coli pathogenic isolates. All the wheys after fermentation with 10% (wt/vol) kefir grains showed inhibition against all 200 isolates. The content of lactic acid bacteria in fermented whey ranged from 1.04 × 107 to 1.17 × 107 CFU/ml and the level of yeasts from 2.05 × 106 to 4.23 × 106 CFU/ml. The main changes in the chemical composition during fermentation were a decrease in lactose content by 41 to 48% along with a corresponding lactic acid production to a final level of 0.84 to 1.20% of the total reaction products. The MIC was a 30% dilution of the fermentation products for most of the isolates, while the MBC varied between 40 and 70%, depending on the isolate. The pathogenic isolates Salmonella enterica serovar Enteritidis 2713 and E. coli 2710 in the fermented whey lost their viability after 2 to 7 h of incubation. When pathogens were deliberately inoculated into whey before fermentation, the CFU were reduced by 2 log cycles for E. coli and 4 log cycles for Salmonella sp. after 24 h of incubation. The inhibition was mainly related to lactic acid production. This work demonstrated the possibility of using kefir grains to ferment an industrial by-product in order to obtain a natural acidic preparation with strong bacterial inhibitory properties that also contains potentially probiotic microorganisms.

D-Lactic Acid Production from Xylose in Engineered Escherichia coli SZ470

2013 ◽  
Vol 641-642 ◽  
pp. 721-724
Author(s):  
Zhao Min Zheng ◽  
Tian Tian ◽  
Jin Hua Wang ◽  
Yong Ze Wang ◽  
Sheng De Zhou
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Acid Production ◽  
Shake Flask ◽  
Lactic Acid Production ◽  
Lactate Production ◽  
Pyruvate Decarboxylase ◽  
Genetically Engineered ◽  
Acetate Production ◽  
A Minor

WD100, knocked out adhE of Escherichia coli SZ470 and inserted ldhA into Escherichia coli WD01, was genetically engineered to utilize xylose. D-lactate production was investigated for shake flask cultures with xylose. In 64h WD100 produce 10.1g/L D-lactate in the shaking flask And it consumed 25g/L xylose during the ending of fermentation.This volumetric productivity with xylose is 0.14 g·L-1·h-1.Because of pyruvate decarboxylase (poxB) expressed in flask fermention,acetate production was up to 4.7g/L.Succinate,formate,ethanol was also produced as a minor product during fermentation.

scholarly journals Production of biopolymer precursors beta-alanine and L-lactic acid from CO2 with metabolically versatile Rhodococcus opacus DSM 43205

2020 ◽  
Author(s):  
Laura Salusjärvi ◽  
Leo Ojala ◽  
Gopal Peddinti ◽  
Michael Lienemann ◽  
Paula Jouhten ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Lactate Dehydrogenase ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Water Electrolysis ◽  
Rhodococcus Opacus ◽  
Beta Alanine ◽  
Polymer Precursors ◽  
Autotrophic Bacteria

AbstractHydrogen oxidizing autotrophic bacteria are promising hosts for CO2 conversion into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium Rhodococcus opacus strain DSM 43205 for synthesis of polymer precursors. Aspartate decarboxylase (panD) or lactate dehydrogenase (ldh) were expressed for beta-alanine or L-lactic acid production, respectively. The heterotrophic cultivations on glucose produced 25 mg L-1 beta-alanine and 742 mg L-1 L-lactic acid, while autotrophic cultivations with CO2, H2 and O2 resulted in the production of 1.8 mg L-1 beta-alanine and 146 mg L-1 L-lactic acid. Beta-alanine was also produced at 345 µg L-1 from CO2 in electrobioreactors, where H2 and O2 were provided by water electrolysis. This work demonstrates that R. opacus DSM 43205 can be readily engineered to produce chemicals from CO2 and provides base for its further metabolic engineering.

scholarly journals Lactic acid production to purification: A review

BioResources ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 4364-4383 ◽  
Author(s):  
Andrea Komesu ◽  
Johnatt Allan Rocha de Oliveira ◽  
Luiza Helena da Silva Martins ◽  
Maria Regina Wolf Maciel ◽  
Rubens Maciel Filho
Keyword(s):  
Lactic Acid ◽  
Renewable Resources ◽  
Building Block ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Department Of Energy ◽  
Pharmaceutical Chemical ◽  
Acid Properties ◽  
Naturally Occurring ◽  
The U.S

Lactic acid is a naturally occurring organic acid that can be used in a wide variety of industries, such as the cosmetic, pharmaceutical, chemical, food, and, most recently, the medical industries. It can be made by the fermentation of sugars obtained from renewable resources, which means that it is an eco-friendly product that has attracted a lot of attention in recent years. In 2010, the U.S. Department of Energy issued a report that listed lactic acid as a potential building block for the future. Bearing the importance of lactic acid in mind, this review summarizes information about lactic acid properties and applications, as well as its production and purification processes.

D-Lactic acid production from Cistus ladanifer residues: Co-fermentation of pentoses and hexoses by Escherichia coli JU15

2022 ◽  
Vol 177 ◽  
pp. 114519
Author(s):  
Júnia Alves-Ferreira ◽  
Florbela Carvalheiro ◽  
Luís C. Duarte ◽  
Ana R.P. Ferreira ◽  
Alfredo Martinez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Cistus Ladanifer

Metabolic engineering of Pichia pastoris for L-lactic acid production using glycerin as carbon source

2015 ◽  
Author(s):  
Nadia Skorupa Parachin ◽  
Pollyne Lima ◽  
Nadielle Melo ◽  
Lucas Carvalho ◽  
Virgililio Castro ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Pichia Pastoris ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production

scholarly journals Recent Advances in D-Lactic Acid Production from Renewable Resources

2019 ◽  
Vol 57 (3) ◽  
pp. 293-304 ◽  
Author(s):  
Maria Alexandri ◽  
Roland Schneider ◽  
Kerstin Mehlmann ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
Renewable Resources ◽  
Acid Production ◽  
Food Packaging ◽  
Lactic Acid Production ◽  
Vital Role ◽  
Promising Alternative ◽  
Current State ◽  
Alternative Feedstocks ◽  
Significant Attention

The production of biodegradable polymers as alternatives to petroleum-based plastics has gained significant attention in the past years. To this end, polylactic acid (PLA) constitutes a promising alternative, finding various applications from food packaging to pharmaceuticals. Recent studies have shown that D-lactic acid plays a vital role in the production of heat-resistant PLA. At the same time, the utilization of renewable resources is imperative in order to decrease the production cost. This review aims to provide a synopsis of the current state of the art regarding D-lactic acid production via fermentation, focusing on the exploitation of waste and byproduct streams. An overview of potential downstream separation schemes is also given. Additionally, three case studies are presented and discussed, reporting the obtained results utilizing acid whey, coffee mucilage and hydrolysate from rice husks as alternative feedstocks for D-lactic acid production.

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