Metabolic engineering as a tool for enhanced lactic acid production

2014 ◽  
Vol 32 (12) ◽  
pp. 637-644 ◽  
Author(s):  
Bikram P. Upadhyaya ◽  
Linda C. DeVeaux ◽  
Lew P. Christopher
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Acid Production ◽  
Lactic Acid Production

scholarly journals Production of biopolymer precursors beta-alanine and L-lactic acid from CO2 with metabolically versatile Rhodococcus opacus DSM 43205

2020 ◽  
Author(s):  
Laura Salusjärvi ◽  
Leo Ojala ◽  
Gopal Peddinti ◽  
Michael Lienemann ◽  
Paula Jouhten ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Lactate Dehydrogenase ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Water Electrolysis ◽  
Rhodococcus Opacus ◽  
Beta Alanine ◽  
Polymer Precursors ◽  
Autotrophic Bacteria

AbstractHydrogen oxidizing autotrophic bacteria are promising hosts for CO2 conversion into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium Rhodococcus opacus strain DSM 43205 for synthesis of polymer precursors. Aspartate decarboxylase (panD) or lactate dehydrogenase (ldh) were expressed for beta-alanine or L-lactic acid production, respectively. The heterotrophic cultivations on glucose produced 25 mg L-1 beta-alanine and 742 mg L-1 L-lactic acid, while autotrophic cultivations with CO2, H2 and O2 resulted in the production of 1.8 mg L-1 beta-alanine and 146 mg L-1 L-lactic acid. Beta-alanine was also produced at 345 µg L-1 from CO2 in electrobioreactors, where H2 and O2 were provided by water electrolysis. This work demonstrates that R. opacus DSM 43205 can be readily engineered to produce chemicals from CO2 and provides base for its further metabolic engineering.

Metabolic engineering of Pichia pastoris for L-lactic acid production using glycerin as carbon source

2015 ◽  
Author(s):  
Nadia Skorupa Parachin ◽  
Pollyne Lima ◽  
Nadielle Melo ◽  
Lucas Carvalho ◽  
Virgililio Castro ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Pichia Pastoris ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production

Metabolic Engineering of Escherichia coli for Lactic Acid Production from Renewable Resources

2017 ◽  
pp. 125-145 ◽  
Author(s):  
Alfredo Martinez ◽  
María E. Rodríguez-Alegría ◽  
Maria Conceição Fernandes ◽  
Guillermo Gosset ◽  
Alejandra Vargas-Tah
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Metabolic Engineering ◽  
Renewable Resources ◽  
Acid Production ◽  
Lactic Acid Production

Improvement of d ‐Lactic Acid Production in Saccharomyces cerevisiae Under Acidic Conditions by Evolutionary and Rational Metabolic Engineering

2017 ◽  
Vol 12 (10) ◽  
pp. 1700015 ◽  
Author(s):  
Seung‐Ho Baek ◽  
Eunice Y. Kwon ◽  
Sang‐Jeong Bae ◽  
Bo‐Ram Cho ◽  
Seon‐Young Kim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Metabolic Engineering ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acidic Conditions

Metabolic engineering approaches for lactic acid production

2006 ◽  
Vol 41 (5) ◽  
pp. 991-1000 ◽  
Author(s):  
Sudheer K. Singh ◽  
Syed U. Ahmed ◽  
Ashok Pandey
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Acid Production ◽  
Lactic Acid Production

scholarly journals Metabolic Engineering ofLactobacillus plantarumfor Directl‐Lactic Acid Production From Raw Corn Starch

2018 ◽  
Vol 13 (5) ◽  
pp. 1700517 ◽  
Author(s):  
Kenji Okano ◽  
Gentaro Uematsu ◽  
Shinji Hama ◽  
Tsutomu Tanaka ◽  
Hideo Noda ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Metabolic Engineering ◽  
Acid Production ◽  
Corn Starch ◽  
Lactic Acid Production
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