Magnetic levitation assisted biofabrication, culture and manipulation of 3D cellular structures using a ring magnet based setup

Author(s):  
Muge Anil‐Inevi ◽  
Kerem Delikoyun ◽  
Gulistan Mese ◽  
H. Cumhur Tekin ◽  
Engin Ozcivici





2015 ◽  
Vol 21 (2) ◽  
pp. 168-176 ◽  
Author(s):  
Li Yang

Purpose – This paper aims to demonstrate the design and verification of a 3D reticulate octahedral cellular structure using both analytical modeling and additive manufacturing. Traditionally, it has been difficult to develop and verify designs for 3D cellular structures due to their design complexity. Design/methodology/approach – Unit cell modeling approach was used to model the octahedral cellular structure. By applying structural symmetry simplification, the cellular structure was simplified into a representative geometry that could be further designed with a standard beam theory. The verification samples were fabricated with EBM process using Ti6Al4V as materials, and compressive testing were performed to evaluate their properties. In addition, designs with different number of unit cells were investigated to evaluate their size effect. Findings – Explicit mechanical property design (including modulus and compressive strength) of the octahedral cellular structure was realized via parametric equations driven by geometrical designs and material types. In addition, it was verified both numerically and experimentally that the octahedral cellular structure exhibit unusual size effect, which is highly predictable. Unlike some of the other cellular structures, the octahedral cellular structure exhibits softening behavior when the number of unit cell increases between the sandwich skins, which could be explained by the upsetting effect commonly observed in bulk deformation processes. Originality/value – This paper established a more comprehensive understanding in the design of octahedral cellular structures, which could enable this type of structure to be designed for sandwich structures with higher fidelity. Therefore, this study not only demonstrated an efficient methodology to design 3D cellular structures using additive manufacturing, but also facilitated the development of design for an additive manufacturing theory.



Author(s):  
Kenichi Arai ◽  
Shintaroh Iwanaga ◽  
Makoto Nakamura


Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Sarah Mishriki ◽  
Srivatsa Aithal ◽  
Tamaghna Gupta ◽  
Rakesh P. Sahu ◽  
Fei Geng ◽  
...  

Fibroblasts (mouse, NIH/3T3) are combined with MDA-MB-231 cells to accelerate the formation and improve the reproducibility of 3D cellular structures printed with magnetic assistance. Fibroblasts and MDA-MB-231 cells are cocultured to produce 12.5 : 87.5, 25 : 75, and 50 : 50 total population mixtures. These mixtures are suspended in a cell medium containing a paramagnetic salt, Gd-DTPA, which increases the magnetic susceptibility of the medium with respect to the cells. A 3D monotypic MDA-MB-231 cellular structure is printed within 24 hours with magnetic assistance, whereas it takes 48 hours to form a similar structure through gravitational settling alone. The maximum projected areas and circularities, and cellular ATP levels of the printed structures are measured for 336 hours. Increasing the relative amounts of the fibroblasts mixed with the MDA-MB-231 cells decreases the time taken to form the structures and improves their reproducibility. Structures produced through gravitational settling have larger maximum projected areas and cellular ATP, but are deemed less reproducible. The distribution of individual cell lines in the cocultured 3D cellular structures shows that printing with magnetic assistance yields 3D cellular structures that resemble in vivo tumors more closely than those formed through gravitational settling. The results validate our hypothesis that (1) fibroblasts act as a “glue” that supports the formation of 3D cellular structures, and (2) the structures are produced more rapidly and with greater reproducibility with magnetically assisted printing than through gravitational settling alone. Printing of 3D cellular structures with magnetic assistance has applications relevant to drug discovery, lab-on-chip devices, and tissue engineering.



2017 ◽  
Vol 124 ◽  
pp. 46-56 ◽  
Author(s):  
Zixing Lu ◽  
Qingsong Wang ◽  
Xiang Li ◽  
Zhenyu Yang


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