Novel Reverse Phase HPLC Method for Simultaneous Determination of Ethynodiol Diacetate (EDA)/Ethinyl Estradiol (EE) in Pharmaceutical Dosage Form

2020 ◽  
Author(s):  
Srinivasa Reddy Chinta ◽  
Vijetha Ponnam ◽  
Naresh Kumar Katari ◽  
Sasikiran Goud Ediga ◽  
Vishnu Murthy Marisetti
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Ethinyl Estradiol ◽  
Hplc Method ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Ethynodiol Diacetate

Simultaneous determination of Sulphadoxine and Pyrimethamine by taking Tolterodine as an internal standard in Pharmaceutical dosage form by RP-HPLC

2021 ◽  
pp. 145-150
Author(s):  
Sushil D. Patil ◽  
Pravin B. Shelke ◽  
Priti Aher ◽  
Maswood Ahmed Hafizur Rahman
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Control Analysis ◽  
Pharmaceutical Dosage Form ◽  
Quality Control Analysis ◽  
Rp Hplc ◽  
Sulphadoxine Pyrimethamine

A simple, rapid, economic, sensitive and precise HPLC method has been developed for the simultaneous determination of Sulphadoxine and Pyrimethamine in pharmaceutical dosage form by taking Tolterodine as an internal standard. The method was carried out using Phenomenex C18 (4.6ID × 250mm; 5µm) column and mobile phase comprised of methanol and Phosphate Buffer in proportion of ratio 60:40 v/v. The flow rate was 1.0mL/min and detection was carried out at 276nm. The retention time of Sulphadoxine, Pyrimethamine and Tolterodine were found to be 2.967, 4.058 and 6.908 respectively. Linearity of Sulphadoxine and Pyrimethamine in the range of 2 to 12μg/mL and 4 to 24μg/mL respectively. The % recoveries of Sulphadoxine and Pyrimethamine were found to be in between 99.93% to 99. 96 % respectively. The proposed method is suitable for the routine quality control analysis for simultaneous determination of Sulphadoxine and Pyrimethamine was in bulk and pharmaceutical dosage form.

Simultaneous determination of metformin and glimepride in pharmaceutical dosage form by reverse-phase liquid chromatography

2005 ◽  
Vol 28 (16) ◽  
pp. 2076-2079 ◽  
Author(s):  
Bhaskar Laxmanrao Kolte ◽  
Bharat Baburao Raut ◽  
Adwait Anant Deo ◽  
Manoj Anil Bagool ◽  
Devanand Baburao Shinde
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Dosage Form ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Reverse Phase Liquid Chromatography ◽  
Phase Liquid

Development and Validation of a Stability-Indicating Reverse Phase HPLC-PDA Method for Determination of Canagliflozin in Bulk and Pharmaceutical Dosage Form

2016 ◽  
Vol 7 (1) ◽  
pp. 54-62 ◽  
Author(s):  
Ishpreet Kaur ◽  
Sharad Wakode ◽  
Harsharan Pal Singh ◽  
Satish Manachanda
Keyword(s):  
Dosage Form ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Development And Validation

scholarly journals Development and Validation of Reverse Phase HPLC Method for Simultaneous Estimation of Allopurinol and Lesinurad in its API and Pharmaceutical Dosage Form

2019 ◽  
Vol 4 (04) ◽  
pp. 50-57
Author(s):  
Sayma Khader ◽  
Ayesha Begum K ◽  
D. Ramakrishna
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Development And Validation ◽  
Tablet Dosage

A new, reliable, and validated reverse phase-high-performance liquid chromatography (HPLC) method was developed to quantify the amount of allopurinol and lesinurad simultaneously in solid (tablet) dosage form. A clear chromatographic division was attained on inertsil ODS (4.6 x 250 mm, 5 mm) column, and a mixture of 0.1% trifluoroacetic acid and methanol in the ratio of 40:60 v/v was used as mobile phase. The rate of flow was set at 1 mL/min, and UV detection was achieved at λmax of 255 nm. Injection volume was set to 20 μL. The correlation coefficient of 0.999 was established, and the accurateness was found to be 100.69 and 100.49 for both the drugs, respectively. Therefore, the developed method was simple, specific, precise, and stable. Hence, the method can be employed to estimate the said drugs in other pharmaceutical formulations.

Simultaneous Determination of Ursodeoxycholic Acid and Chenodeoxycholic Acid in Pharmaceutical Dosage Form by HPLC–UV Detection

2017 ◽  
Vol 100 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Mostafa A Khairy ◽  
Fotouh R Mansour
Keyword(s):  
Ursodeoxycholic Acid ◽  
Simultaneous Determination ◽  
Chenodeoxycholic Acid ◽  
Dosage Form ◽  
Reference Method ◽  
Reversed Phase ◽  
Hplc Method ◽  
Uv Detection ◽  
Pharmaceutical Dosage Form

Abstract A reversed-phase HPLC method was developed for the simultaneous determination of ursodeoxycholic acid (UDCA) and the epimeric isomer, chenodeoxycholic acid (CDCA), in their synthetic mixtures and in tablet dosage form. The proposed HPLC method uses a C18 column and mobile phase consisting of an acetonitrile–phosphate buffer mixture (pH 2.3, 100 mM; 50 + 50, v/v) at a flow rate of 2.0 mL/min with UV detection at 210 nm. The method was validated according to the International Conference on Harmonization guidelines; and linearity, range,accuracy, precision, robustness,and system suitability were studied. The LOD and LOQ were also calculated and found to be 1.23 and 3.73 μg/mL for UDCA and 0.83 and 2.52 μg/mL for CDCA, respectively. The method was adapted for UHPLC, in which baseline separation was achieved in <2.5 min. The assay results of Ursomix tablets by the developed method were statistically compared with those obtained by the reference method using t- and F-tests, and no significant differences were observed.

scholarly journals Analytical Method Development and validation for Simultaneous Determination of Atenolol and Nitrendipine in Pharmaceutical Dosage Form by RP-HPLC Method

2016 ◽  
Vol 03 (01) ◽  
pp. 14-22
Author(s):  
Prathap Boobalan ◽  
Srinivasa Rao Gorantla ◽  
Gobalakrishnan Pandurangan ◽  
Venkata Swathikrishna Kavuluru ◽  
Priyanka Vadthey
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Dosage Form ◽  
Method Development ◽  
Hplc Method ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc ◽  
Method Development And Validation ◽  
Development And Validation
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