Sensitive, wide‐range and high‐throughput quantification of cyclosporine in whole blood using ultra‐performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody‐conjugated magnetic immunoassay

2021 ◽  
Author(s):  
Takuma Watanabe ◽  
Ryota Tanaka ◽  
Hiroyuki Ono ◽  
Yosuke Suzuki ◽  
Ryosuke Tatsuta ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Throughput ◽  
Whole Blood ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Wide Range

Simple high-throughput analytical method using ultra-performance liquid chromatography coupled with tandem mass spectrometry to quantify total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in urine

2015 ◽  
Vol 53 (8) ◽  
Author(s):  
Jun-Young Yang ◽  
Hyun-Kyong Ahn ◽  
Si-Won Lee ◽  
You-Jung Han ◽  
Young-Jun Oh ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Throughput ◽  
Tobacco Smoke ◽  
Analytical Method ◽  
Ultra Performance Liquid Chromatography ◽  
Urinary Concentration ◽  
Tandem Mass ◽  
Biomarker Of Exposure

AbstractSince the urinary concentration of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) is a reliable biomarker of exposure to tobacco smoke, we developed a relatively simple high-throughput chromatographic method to quantify total urinary NNAL concentrations in the general population.The high-throughput analytical method was developed using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) to identify and quantify total urinary NNAL concentrations in 10 non-smokers and 15 otherwise healthy smokers.Loss of nitric oxide atAn UPLC-MS/MS analytical method to quantify total urinary NNAL concentrations in smokers that does not require sample derivatization is presented herein. The method could be useful in clarifying the toxicities associated with human exposure to cigarette smoking. However, quantification might be adversely affected by co-eluting interfering compounds or selective ion suppression or enhancement as a result of having only one ion transition to monitor NNAL and NNAL-methyl-

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