Efficient and easily scalable protein folding strong anion exchange chromatography for renaturation and simultaneous purification of recombinant human asparaginase from E. coli

2018 ◽  
Vol 34 (4) ◽  
pp. 1036-1044 ◽  
Author(s):  
Rajesh Kumar Kante ◽  
Sandeep Vemula ◽  
Maheswara Reddy Mallu ◽  
Srinivasa Reddy Ronda
Keyword(s):  
Protein Folding ◽  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
E Coli ◽  
Strong Anion Exchange

scholarly journals Heparin Isomeric Oligosaccharide Separation Using Volatile Salt Strong Anion Exchange Chromatography

2016 ◽  
Vol 88 (23) ◽  
pp. 11542-11550 ◽  
Author(s):  
Rebecca L. Miller ◽  
Scott E. Guimond ◽  
Maitreyi Shivkumar ◽  
Jemma Blocksidge ◽  
James A. Austin ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Strong Anion Exchange

scholarly journals Biodiversity Of Bacteriophages From Lysed Batch Culture Of Recombinant Escherichia Coli BL21(DE3) Examined By Anion Exchange Chromatography And TEM

2020 ◽  
Author(s):  
Alla Kushkina ◽  
Fedor Tovkach
Keyword(s):  
Anion Exchange ◽  
Batch Culture ◽  
Restriction Analysis ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Structural Defects ◽  
E Coli ◽  
Main Portion ◽  
Phage Capsid ◽  
Phage Population

AbstractIt was found that the phage population assaulted a batch culture of an industrial recombinant derivative of E. coli BL21(DE3) was attenuated that manifested in producing pinprick-type plaques and their inability to propagation in subsequent passages. Because of this, the goals of the present research were to evaluate biodiversity and scrutinize the possible virion structural defects of the attenuated phage population prior to pure phage lines isolation. The anion exchange chromatography (AEC) was chosen as the principal method allowing to get a comparative phage population profile based on the virion net surface charge, as well as to treat the 5-liter virion-contained sample and collect high-quality concentrated and separated phage fractions to further analysis. The isolate consisted of a mix of two phages belonging to Myoviridae (A2-morphotype) and Siphoviridae (B1-morphotype) families. By restriction analysis, the main portion of this phage pool (about 99% of all virions) was identified as the primary population of myophage Lw1, which possessed its own intra-population biodiversity (heterogeneity). It consisted of a major and two minor subpopulations that differed by phage capsid size and shape. The subpopulation III consisted of aberrant tubby-phages with triprolate (expanded at both sides) capsids having low aspect ratio.

Characterization of Low-Molecular-Weight Heparins by Strong Anion-Exchange Chromatography

2017 ◽  
Vol 100 (6) ◽  
pp. 1706-1714 ◽  
Author(s):  
Radosław Sadowski ◽  
Renata Gadzała-Kopciuch ◽  
Tomasz Kowalkowski ◽  
Paweł Widomski ◽  
Ludwik Jujeczka ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Quantitative Composition ◽  
Low Molecular Weight ◽  
Strong Anion Exchange ◽  
Post Hoc ◽  
First Time

Abstract Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest. In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin). For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs. In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman–Keuls post hoc test).

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