scholarly journals Sequential enrichment of sulfated glycans by strong anion-exchange chromatography prior to mass spectrometric measurements

2010 ◽  
Vol 21 (3) ◽  
pp. 348-357 ◽  
Author(s):  
Ming Lei ◽  
Milos V. Novotny ◽  
Yehia Mechref
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Mass Spectrometric ◽  
Strong Anion Exchange ◽  
Spectrometric Measurements

scholarly journals Heparin Isomeric Oligosaccharide Separation Using Volatile Salt Strong Anion Exchange Chromatography

2016 ◽  
Vol 88 (23) ◽  
pp. 11542-11550 ◽  
Author(s):  
Rebecca L. Miller ◽  
Scott E. Guimond ◽  
Maitreyi Shivkumar ◽  
Jemma Blocksidge ◽  
James A. Austin ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Strong Anion Exchange

Characterization of Low-Molecular-Weight Heparins by Strong Anion-Exchange Chromatography

2017 ◽  
Vol 100 (6) ◽  
pp. 1706-1714 ◽  
Author(s):  
Radosław Sadowski ◽  
Renata Gadzała-Kopciuch ◽  
Tomasz Kowalkowski ◽  
Paweł Widomski ◽  
Ludwik Jujeczka ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Quantitative Composition ◽  
Low Molecular Weight ◽  
Strong Anion Exchange ◽  
Post Hoc ◽  
First Time

Abstract Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest. In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin). For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs. In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman–Keuls post hoc test).

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