Assessment of Shock Pretreatment and Alkali Pretreatment on Corn Stover Using Enzymatic Hydrolysis

2021 ◽  
Author(s):  
Opeyemi Olokede ◽  
Shen‐chun Hsu ◽  
Simon Schiele ◽  
Huang Ju ◽  
Mark Holtzapple
2010 ◽  
Vol 162 (2) ◽  
pp. 390-390
Author(s):  
Xun He ◽  
Yelian Miao ◽  
Xuejian Jiang ◽  
Zidong Xu ◽  
Pingkai Ouyang

2009 ◽  
Vol 160 (8) ◽  
pp. 2449-2457 ◽  
Author(s):  
Xun He ◽  
Yelian Miao ◽  
Xuejian Jiang ◽  
Zidong Xu ◽  
Pingkai Ouyang

2020 ◽  
Vol 204 ◽  
pp. 106407 ◽  
Author(s):  
Shengxin An ◽  
Wenzhi Li ◽  
Fengyang Xue ◽  
Xu Li ◽  
Ying Xia ◽  
...  

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 494 ◽  
Author(s):  
Kang Hyun Lee ◽  
Ye Won Jang ◽  
Jeongho Lee ◽  
Seunghee Kim ◽  
Chulhwan Park ◽  
...  

Biorefinery, which utilizes carbon-neutral biomass as a resource, is attracting attention as a significant alternative in a modern society confronted with climate change. In this study, spent coffee grounds (SCGs) were used as the feedstock for lactic acid fermentation. In order to improve sugar conversion, alkali pretreatment was optimized by a statistical method, namely response surface methodology (RSM). The optimum conditions for the alkali pretreatment of SCGs were determined as follows: 75 °C, 3% potassium hydroxide (KOH) and a time of 2.8 h. The optimum conditions for enzymatic hydrolysis of pretreated SCGs were determined as follows: enzyme complex loading of 30-unit cellulase, 15-unit cellobiase and 50-unit mannanase per g biomass and a reaction time of 96 h. SCG hydrolysates were used as the carbon source for Lactobacillus cultivation, and the conversions of lactic acid by L. brevis ATCC 8287 and L. parabuchneri ATCC 49374 were 40.1% and 55.8%, respectively. Finally, the maximum lactic acid production by L. parabuchneri ATCC 49374 was estimated to be 101.2 g based on 1000 g of SCGs through the optimization of alkali pretreatment and enzymatic hydrolysis.


2019 ◽  
Vol 11 (11) ◽  
pp. 5847-5856 ◽  
Author(s):  
Ju Chen ◽  
Kokou Adjallé ◽  
Thanh Tung Lai ◽  
Simon Barnabé ◽  
Michel Perrier ◽  
...  

2017 ◽  
Vol 60 (4) ◽  
pp. 1025-1033
Author(s):  
Alicia A. Modenbach ◽  
Sue E. Nokes ◽  
Michael D. Montross ◽  
Barbara L. Knutson

Abstract. High-solids lignocellulosic pretreatment using NaOH followed by high-solids enzymatic hydrolysis was evaluated for an on-farm biochemical conversion process. Increasing the solids loadings for these processes has the potential for increasing glucose concentrations and downstream ethanol production; however, sequential processing at high-solids loading similar to an on-farm cellulose conversion system has not been studied. This research quantified the effects of high-solids pretreatment with NaOH and subsequent high-solids enzymatic hydrolysis on cellulose conversion. As expected, conversion efficiency was reduced; however, the highest glucose concentration (40.2 g L-1), and therefore the highest potential ethanol concentration, resulted from the high-solids combined pretreatment and hydrolysis. Increasing the enzyme dosage improved cellulose conversion from 9.6% to 36.8% when high-solids loadings were used in both unit operations; however, increasing NaOH loading and pretreatment time did not increase the conversion efficiency. The enzyme-to-substrate ratio had a larger impact on cellulose conversion than the NaOH pretreatment conditions studied, resulting in recommendations for an on-farm bioconversion system. Keywords: Corn stover, Enzymatic hydrolysis, Enzyme loading, High solids, Low solids, Sodium hydroxide.


2018 ◽  
Vol 124 ◽  
pp. 201-208 ◽  
Author(s):  
Jia-Qing Zhu ◽  
Wen-Chao Li ◽  
Lei Qin ◽  
Xiong Zhao ◽  
Si Chen ◽  
...  

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