scholarly journals Efflux Pumps Might Not Be the Major Drivers of QAC Resistance in Methicillin-Resistant Staphylococcus aureus

ChemBioChem ◽  
2017 ◽  
Vol 18 (16) ◽  
pp. 1573-1577 ◽  
Author(s):  
Megan C. Jennings ◽  
Megan E. Forman ◽  
Stephanie M. Duggan ◽  
Kevin P. C. Minbiole ◽  
William M. Wuest
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pumps ◽  
Methicillin Resistant

Inside Cover: Efflux Pumps Might Not Be the Major Drivers of QAC Resistance in Methicillin-Resistant Staphylococcus aureus (ChemBioChem 16/2017)

ChemBioChem ◽  
2017 ◽  
Vol 18 (16) ◽  
pp. 1560-1560
Author(s):  
Megan C. Jennings ◽  
Megan E. Forman ◽  
Stephanie M. Duggan ◽  
Kevin P. C. Minbiole ◽  
William M. Wuest
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pumps ◽  
Methicillin Resistant

scholarly journals Fluoroquinolone resistant mechanisms in methicillin-resistant Staphylococcus aureus clinical isolates in Cairo, Egypt

2013 ◽  
Vol 7 (11) ◽  
pp. 796-803 ◽  
Author(s):  
Rasha A Hashem ◽  
Aymen S Yassin ◽  
Hamdallah H Zedan ◽  
Magdy A Amin
Keyword(s):  
Health Care ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pump ◽  
Efflux Pumps ◽  
Clinical Isolates ◽  
Local Health ◽  
Methicillin Resistant ◽  
Persistent Problem ◽  
Health Care Settings

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a persistent problem in community and health care settings. Fluoroquinolones are among the drugs of choice used to treat MRSA infections. This study aims to identify different mechanisms of fluoroquinolne resistance in local MRSA random sampling isolates in Cairo, Egypt. Methodology: A total of 94 clinical isolates of S. aureus were collected from two major University hospitals in Cairo. Identification was confirmed by appropriate morphological, cultural, and biochemical tests. The antibiotic susceptibility pattern was determined for all isolates. The possible involvement of efflux pumps in mediating fluoroquinolone resistance as well as changes in the quinolone resistance determining region (QRDR) of gyrA and gyrB genes were investigated Results: A total of 45 isolates were found to be MRSA, among which 26 isolates were found to be fluoroquinolone-resistant. The MIC values of the tested fluoroquinolones in the presence of the efflux pump inhibitors omeprazole and piperine were reduced. Measuring the uptake of ciprofloxacin upon the addition of the efflux pump inhibitor omeprazole, an increased level of accumulation was observed. Non-synonymous and silent mutations were detected in the QRDR of gyrA and gyrB genes. Conclusions: These results shed light on some of the resistance patterns of MRSA strains isolated from local health care settings in Cairo, Egypt. The resistance of these MRSA towards fluoroquinolones does not depend only on mutation in target genes; other mechanisms of resistance such as the permeability effect, efflux pumps and decreased availability of quinolones at the target site can also be involved.

scholarly journals Distribution of Antiseptic Resistance GenesqacA, qacB, andsmrin Methicillin-Resistant Staphylococcus aureus Isolated in Toronto, Canada, from 2005 to 2009

2011 ◽  
Vol 55 (6) ◽  
pp. 2999-3001 ◽  
Author(s):  
Jean Longtin ◽  
Christine Seah ◽  
Krystal Siebert ◽  
Allison McGeer ◽  
Andrew Simor ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Intensive Care ◽  
Intensive Care Units ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pumps ◽  
Chlorhexidine Gluconate ◽  
Methicillin Resistant ◽  
Content Type ◽  
Genes Encoding

ABSTRACTDecreased susceptibility to chlorhexidine gluconate (CHDN) in methicillin-resistantStaphylococcus aureus(MRSA) is associated with theqacA,qacB, andsmrgenes, encoding efflux pumps. A total of 334 MRSA isolates were collected from two Canadian intensive care units between 2005 and 2009. We identified theqacABgenes in 7 strains (2%; 2qacAgenes and 5qacBgenes) and thesmrgene in 23 (7%) strains. CHDN minimal bactericidal concentrations were slightly higher for strains harboringsmrgenes.

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