Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR-Cas9 Activation

ChemBioChem ◽  
2018 ◽  
Vol 19 (16) ◽  
pp. 1716-1719 ◽  
Author(s):  
Yee Hwee Lim ◽  
Fong Tian Wong ◽  
Wan Lin Yeo ◽  
Kuan Chieh Ching ◽  
Yi Wee Lim ◽  
...  
2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Lingjuan Jiang ◽  
Lu Wang ◽  
Jihui Zhang ◽  
Hao Liu ◽  
Bin Hong ◽  
...  

2013 ◽  
Vol 67 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Wei-Ting Liu ◽  
Anne Lamsa ◽  
Weng Ruh Wong ◽  
Paul D Boudreau ◽  
Roland Kersten ◽  
...  

2016 ◽  
Vol 82 (6) ◽  
pp. 1898-1905 ◽  
Author(s):  
Peng-Hui Yuan ◽  
Ri-Cheng Zhou ◽  
Xuepeng Chen ◽  
Shuai Luo ◽  
Feng Wang ◽  
...  

ABSTRACTDaptomycin is a potent cyclic lipopeptide antibiotic. It is widely used against various Gram-positive bacterial pathogens. Historically, a poor understanding of the transcriptional regulation of daptomycin biosynthesis has limited the options for targeted genetic engineering toward titer improvement. Here, we isolated a TetR family transcriptional regulator, DepR1, from the industrial producerStreptomyces roseosporusSW0702 using a biotinylateddptEpromoter (dptEp) as a probe. The direct interaction between DepR1 anddptEpthen was confirmed by electrophoretic mobility shift assays and DNase I footprinting assays. The deletion ofdepR1led to a reduction indptEpactivity and the cessation of daptomycin production. TheΔdepR1mutant produced less red pigment and failed to sporulate on R5 medium. This suggests that DepR1 plays a positive role in the control of morphological differentiation. Moreover, DepR1 was positively autoregulated by directly binding to its own promoter. This might account for the positive feedback regulation of daptomycin production. Based on these positive effects, genetic engineering by overexpression ofdepR1raised daptomycin production and shortened the fermentation period both in flask and in fermentor.


2014 ◽  
Vol 106 ◽  
pp. 260-269 ◽  
Author(s):  
Guojian Liao ◽  
Longxiang Xie ◽  
Xin Li ◽  
Zhongyi Cheng ◽  
Jianping Xie

2010 ◽  
Vol 163 (4) ◽  
pp. 453-462 ◽  
Author(s):  
Wenyu Lu ◽  
Jinghua Fan ◽  
Jianping Wen ◽  
Zhendong Xia ◽  
Qinggele Caiyin

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