Mutants of Streptomyces roseosporus that express enhanced recombination within partially homologous genes

Abstract Background Night-break (NB) has been proven to repress flowering of short-day plants (SDPs). Long-noncoding RNAs (lncRNAs) play key roles in plant flowering. However, investigation of the relationship between lncRNAs and NB responses is still limited, especially in Chenopodium quinoa, an important short-day coarse cereal. Results In this study, we performed strand-specific RNA-seq of leaf samples collected from quinoa seedlings treated by SD and NB. A total of 4914 high-confidence lncRNAs were identified, out of which 91 lncRNAs showed specific responses to SD and NB. Based on the expression profiles, we identified 17 positive- and 7 negative-flowering lncRNAs. Co-expression network analysis indicated that 1653 mRNAs were the common targets of both types of flowering lncRNAs. By mapping these targets to the known flowering pathways in model plants, we found some pivotal flowering homologs, including 2 florigen encoding genes (FT (FLOWERING LOCUS T) and TSF (TWIN SISTER of FT) homologs), 3 circadian clock related genes (EARLY FLOWERING 3 (ELF3), LATE ELONGATED HYPOCOTYL (LHY) and ELONGATED HYPOCOTYL 5 (HY5) homologs), 2 photoreceptor genes (PHYTOCHROME A (PHYA) and CRYPTOCHROME1 (CRY1) homologs), 1 B-BOX type CONSTANS (CO) homolog and 1 RELATED TO ABI3/VP1 (RAV1) homolog, were specifically affected by NB and competed by the positive and negative-flowering lncRNAs. We speculated that these potential flowering lncRNAs may mediate quinoa NB responses by modifying the expression of the floral homologous genes. Conclusions Together, the findings in this study will deepen our understanding of the roles of lncRNAs in NB responses, and provide valuable information for functional characterization in future.

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Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles

Scientific Reports ◽

10.1038/s41598-021-82343-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Qiaoli Chen ◽

Ruizhi Zhang ◽

Danlei Li ◽

Feng Wang

Keyword(s):

Fat Metabolism ◽

Bursaphelenchus Xylophilus ◽

Pinewood Nematode ◽

Genetic Characteristics ◽

Highest Weight ◽

Homologous Genes ◽

Third Stage ◽

Highly Correlated ◽

Oil Red O Staining ◽

Oil Red O

AbstractThe third-stage dispersal juvenile (DJ3) of pinewood nematode (PWN) is highly associated with low-temperature survival and spread of the nematode. Oil-Red-O staining showed that its lipid content was significantly higher compared with other PWN stages. Weighted gene coexpression network analysis identified that genes in the pink module were highly related to DJ3 induced in the laboratory (DJ3-lab). These genes were arranged according to their gene significance (GS) to DJ3-lab. Of the top 30 genes with the highest GS, seven were found to be highly homologous to the cysteine protease family cathepsin 1 (CATH1). The top 30 genes with the highest weight value to each of the seven genes in the pink module were selected, and finally 35 genes were obtained. Between these seven CATH1 homologous genes and their 35 highly related genes, 15 were related to fat metabolism or autophagy. These autophagy-related genes were also found to be highly correlated with other genes in the pink module, suggesting that autophagy might be involved in the mechanism of longevity in DJ3 and the formation of DJ3 by regulating genes related to fat metabolism.

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Construction of hybrid xylE genes between the two duplicate homologous genes from TOL plasmid pWW53: comparison of the kinetic properties of the gene products

Journal of General Microbiology ◽

10.1099/00221287-136-8-1583 ◽

1990 ◽

Vol 136 (8) ◽

pp. 1583-1589 ◽

Cited By ~ 12

Author(s):

P. A. Williams ◽

S. J. Assinder ◽

L. E. Shaw

Keyword(s):

Kinetic Properties ◽

Gene Products ◽

Tol Plasmid ◽

Homologous Genes

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Isolation and Analysis of Cinnamic Acid 4-Hydroxylase Homologous Genes from a Hybrid Aspen,Populus kitakamiensis

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.60.1586 ◽

1996 ◽

Vol 60 (10) ◽

pp. 1586-1597 ◽

Cited By ~ 24

Author(s):

Shinya Kawai ◽

Ariko Mori ◽

Takahiro Shiokawa ◽

Shinya Kajita ◽

Yoshihiro Katayama ◽

...

Keyword(s):

Cinnamic Acid ◽

Hybrid Aspen ◽

Homologous Genes

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Prevalence of two homologous genes encoding glycosyltransferases of Helicobacter pylori in the United States and Japan

Journal of Gastroenterology and Hepatology ◽

10.1111/j.1440-1746.2011.06779.x ◽

2011 ◽

pp. no-no ◽

Cited By ~ 1

Author(s):

Miyuki Matsuda ◽

Seiji Shiota ◽

Osamu Matsunari ◽

Masahide Watada ◽

Kazunari Murakami ◽

...

Keyword(s):

United States ◽

Helicobacter Pylori ◽

The United States ◽

Homologous Genes ◽

Genes Encoding

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Quantitating Aortic Atherosclerosis in Rabbits and Mice

Microscopy and Microanalysis ◽

10.1017/s1431927600008473 ◽

1997 ◽

Vol 3 (S2) ◽

pp. 317-318

Author(s):

David A. Sanan ◽

Dale L. Newland

Keyword(s):

Gene Knockout ◽

Laboratory Mouse ◽

Lipoprotein Metabolism ◽

Wild Type Mouse ◽

Mouse Strains ◽

Chow Diet ◽

Homologous Genes ◽

Normal Chow ◽

Metabolism Gene ◽

Knockout Technology

Build-up of visible atherosclerotic plaque in the arteries is readily quantifiable. The mouse and the rabbit provide useful models for understanding the pathogenesis of atherosclerosis by investigating the effects of genetic and dietary perturbations.Although the wild type mouse does not develop atherosclerosis, atherosclerosis susceptibility genes have been identified in some laboratory mouse strains which do. Furthermore, transgenic technology and gene targeting have produced genetically modified mice that express various apolipoproteins, enzymes and cofactors involved in human lipoprotein metabolism. Gene “knockout” technology allows transgene expression without interference from homologous genes. One notable “knockout” mouse, deficient in apolipoprotein E, develops spontaneous atherosclerosis on a normal chow diet. Transgenic modulations of the atherosclerotic responses of these highly susceptible mice are more pronounced and easily measured. Small, cheap and fast breeding, mice are convenient animal models. But to make mice susceptible to atherosclerosis, their genetic background has to be so drastically altered that the resulting lipoprotein metabolism may not model the human metabolism accurately enough.

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Localization, turnover and conservation of gp15/400 in different stages ofBrugia malayi

Parasitology ◽

10.1017/s0031182000067810 ◽

1993 ◽

Vol 107 (4) ◽

pp. 449-457 ◽

Cited By ~ 10

Author(s):

M. E. Selkirk ◽

W. F. Gregory ◽

R. E. Jenkins ◽

R. M. Maizels

Keyword(s):

Protein Complex ◽

Cdna Probe ◽

The Body ◽

Mammalian Host ◽

Major Protein ◽

Homologous Genes ◽

Acanthocheilonema Viteae ◽

Filarial Nematodes ◽

The Matrix

SUMMARYThe expression of a protein complex designated gp15/400, previously identified via extrinsic iodination of adultBrugia malayi, was examined by labelling all stages found in the mammalian host and immunoprecipitation with a specific antibody raised to a recombinant protein. In this way, gp15/400 could be detected in L3, L4, adult worms and microfilariae recovered from jirds and labelled with Bolton–Hunter reagent. Metabolic labelling indicated that gp15/400 was released into culture medium when adult worms were maintainedin vitro, but at a rate slower than that of gp29, the major soluble cuticular glycoprotein. Immuno-electron microscopy showed that the protein complex was broadly distributed in different tissues, although it was not detectable in the cuticle of adult worms. Dense labelling was observed in the matrix of the basal laminae bordering the hypodermis, somatic musculature and oesophagus, and lower but significant labelling was seen in the cells overlying these extracellular matrices. Hybridization of genomic DNA with a cDNA probe encoding gp15/400 indicated that homologous genes were present inDirofilaria immitisandAcanthocheilonema viteae. The failure to detect related genes in non-filarial nematodes was presumed to be due to divergence beyond the practical limits of detection by nucleic acid probes, as antibody reagents showed that the protein cross-reacted immunologically with ABA-1, a major protein allergen from the body fluid ofAscaris.

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Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1421475112 ◽

2015 ◽

Vol 112 (18) ◽

pp. 5850-5855 ◽

Cited By ~ 82

Author(s):

Yongli Qiao ◽

Jinxia Shi ◽

Yi Zhai ◽

Yingnan Hou ◽

Wenbo Ma

Keyword(s):

Small Rna ◽

Rna Silencing ◽

Effector Proteins ◽

Helicase Domain ◽

Small Interfering Rnas ◽

Developmental Defects ◽

Interacting Protein ◽

Homologous Genes ◽

Unidentified Component ◽

Virulence Target

A broad range of parasites rely on the functions of effector proteins to subvert host immune response and facilitate disease development. The notorious Phytophthora pathogens evolved effectors with RNA silencing suppression activity to promote infection in plant hosts. Here we report that the Phytophthora Suppressor of RNA Silencing 1 (PSR1) can bind to an evolutionarily conserved nuclear protein containing the aspartate–glutamate–alanine–histidine-box RNA helicase domain in plants. This protein, designated PSR1-Interacting Protein 1 (PINP1), regulates the accumulation of both microRNAs and endogenous small interfering RNAs in Arabidopsis. A null mutation of PINP1 causes embryonic lethality, and silencing of PINP1 leads to developmental defects and hypersusceptibility to Phytophthora infection. These phenotypes are reminiscent of transgenic plants expressing PSR1, supporting PINP1 as a direct virulence target of PSR1. We further demonstrate that the localization of the Dicer-like 1 protein complex is impaired in the nucleus of PINP1-silenced or PSR1-expressing cells, indicating that PINP1 may facilitate small RNA processing by affecting the assembly of dicing complexes. A similar function of PINP1 homologous genes in development and immunity was also observed in Nicotiana benthamiana. These findings highlight PINP1 as a previously unidentified component of RNA silencing that regulates distinct classes of small RNAs in plants. Importantly, Phytophthora has evolved effectors to target PINP1 in order to promote infection.

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