The combination of miR-122 overexpression and Let-7f silencing induces hepatic differentiation of adipose tissue-derived stem cells

2017 ◽  
Vol 41 (10) ◽  
pp. 1083-1092 ◽  
Author(s):  
Nahid Davoodian ◽  
Abbas S. Lotfi ◽  
Masoud Soleimani ◽  
Hori Ghaneialvar
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Hepatic Differentiation

Let-7f microRNA negatively regulates hepatic differentiation of human adipose tissue-derived stem cells

2014 ◽  
Vol 70 (3) ◽  
pp. 781-789 ◽  
Author(s):  
Nahid Davoodian ◽  
Abbas S. Lotfi ◽  
Masoud Soleimani ◽  
Seyed Javad Mola ◽  
Sare Arjmand
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Hepatic Differentiation

MicroRNA-122 Overexpression Promotes Hepatic Differentiation of Human Adipose Tissue-Derived Stem Cells

2014 ◽  
Vol 115 (9) ◽  
pp. 1582-1593 ◽  
Author(s):  
Nahid Davoodian ◽  
Abbas S. Lotfi ◽  
Masoud Soleimani ◽  
Seyed Javad Mowla
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Hepatic Differentiation

Development of Simple Protocol for Generation of Functionally Active Hepatocyte-like Cells from Human Adipose Tissue-derived Stem Cells

2019 ◽  
Author(s):  
Sahere Rouzbehan ◽  
Nahid Davoodian ◽  
Ali Jamshidi ◽  
Ali Atashabparvar ◽  
Najmeh Davoodian
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Growth Factor ◽  
Human Adipose Tissue ◽  
Culture Conditions ◽  
Alpha Fetoprotein ◽  
Oncostatin M ◽  
Hepatic Differentiation ◽  
Differentiated Cells ◽  
Simple Protocol

Background and Aims: Human adipose tissue-derived stem cells (hASCs) are considered as an attractive source of regenerative stem cells, mainly because of their higher proliferation rate, more accessibility and hepatocyte like properties as compared to mesenchymal stem cells isolated from other tissues. Numerous studies have described the beneficial use of adipose tissue-derived stem cells for generating hepatocyte-like cells. However, due to the lack of appropriate culture conditions, most of the produced cells exhibit poor functionality. The aim of the present study was to establish a new protocol for the efficient hepatic differentiation of hASCs. Materials and Methods: hASCs were cultured in hepatic differentiation medium containing fibroblast growth factor 4, hepatocyte growth factor, dexamethasone and oncostatin M using a three-step protocol up to 21 days. Then, the functionality of the treated cells was evaluated by analyzing specific hepatocyte genes and biochemical markers at various time points. Results: A significant upregulation in albumin, alpha-fetoprotein, cytokeratin 18 and hepatocyte nuclear factor-4α expressions was observed in differentiated cells relative to day 1 of differentiation protocol. Moreover, the finding of glycogen deposits increased urea production and positive immunofluorescence staining for albumin and alpha-fetoprotein in hepatocyte-like cells suggesting that most of the cells differentiate into hepatocyte-like cells. Conclusions: Our report has provided a simple protocol for differentiation of hASCs into more functional hepatocyte-like cells.

Transcriptional characterization of Wnt pathway during sequential hepatic differentiation of human embryonic stem cells and adipose tissue-derived stem cells

2013 ◽  
Vol 434 (2) ◽  
pp. 235-240 ◽  
Author(s):  
Jeonghoon Heo ◽  
Eun-Kyung Ahn ◽  
Hae-Gyeong Jeong ◽  
Young-Ho Kim ◽  
Sun-Hee Leem ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Wnt Pathway ◽  
Embryonic Stem ◽  
Hepatic Differentiation

Hepatic differentiation of human adipose tissue-derived mesenchymal stem cells and adverse effects of arsanilic acid and acetaminophen during in vitro hepatic developmental stage

2015 ◽  
Vol 31 (3) ◽  
pp. 149-159 ◽  
Author(s):  
Mi-Jeong Kwon ◽  
Seok-Jin Kang ◽  
Young-Il Park ◽  
Yool-Hee Yang ◽  
Sa-Ik Bang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Adverse Effects ◽  
Developmental Stage ◽  
Human Adipose Tissue ◽  
Hepatic Differentiation ◽  
Arsanilic Acid

Insulin‐like growth factor‐I promotes hepatic differentiation of human adipose tissue‐derived stem cells

2019 ◽  
Vol 43 (5) ◽  
pp. 476-485 ◽  
Author(s):  
Zahra Shabani Azandaryani ◽  
Nahid Davoodian ◽  
Afshin Samiei ◽  
Sahere Rouzbehan
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Growth Factor ◽  
Human Adipose Tissue ◽  
Insulin Like Growth Factor ◽  
Hepatic Differentiation ◽  
Factor I ◽  
Growth Factor I

Adipose tissue-derived stem cells versus bone marrow-derived stem cells as an angiogenic therapy after ischemic limb injury in the adult male albino rat

2018 ◽  
Vol 41 (1) ◽  
pp. 172-191
Author(s):  
Mohamed Mohamed ◽  
Mona Ali ◽  
Maha Abo-gazia ◽  
Rania Galhom ◽  
Amira farage
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Adult Male ◽  
Albino Rat ◽  
Angiogenic Therapy ◽  
Ischemic Limb ◽  
Limb Injury

Comparison of the Effect of Adipocyte-derived Stem Cells and Curcumin Nanoliposomes with Phenytoin on Open Cutaneous Wound Healing in Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Mohammadreza Ebrahimzade ◽  
Mohammad Mirdoraghi ◽  
Ameneh Alikarami ◽  
Sahar Heidari ◽  
Tayebeh Rastegar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Tissue ◽  
Healing Time ◽  
Daily Work ◽  
Expression Of Genes ◽  
Skin Ulcers ◽  
Immobility Time ◽  
Liposomal Nanoparticles ◽  
Chromosome Staining

Background: Reducing the healing time of wounds can decrease the patient`s immobility time and their medical costs,leading a faster return of the patients to daily work. Objective: To compare the effect of adipose-derived stem cells and curcumin-containing liposomal nanoparticles with phenytoin on wound healing. Method: After anesthesia of the rats, open skin ulcers were made by a bistoury blade.Subsequently,stem cells were re-moved from the adipose tissue of theupper border of the epididymis. Then,the originality of stem cells was confirmed by the flow cytometry. The fusion method was used to prepare the liposome;and also nanoliposomal particles wereconfirmedby using the DLS microscope.The percentage of recovery and the cell count was measured with IMAGEJ.The expression of genes was assessed by PCR. The number of fibro blasts was counted by immuno histo chemistry techniques.The amount of collagen was determined by Tri-chromosome staining and the number of capillaries was enumerated byH & E staining. Results: The expression of TGF-β1 gene, vascular number, wound healing rate and the numberof fibroblasts increased significantly in adipose tissue-derived stem cells and curcumin nanoliposome groups(p<0.05);the wound surface was also decreased significantly(p<0.05). Conclusion: Based on the results of our research, adipose tissue-derived stem cells and curcumin nanoliposomescan heal wounds efficiently.

scholarly journals Comparative analysis of mouse bone marrow and adipose tissue mesenchymal stem cells for critical limb ischemia cell therapy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pegah Nammian ◽  
Seyedeh-Leili Asadi-Yousefabad ◽  
Sajad Daneshi ◽  
Mohammad Hasan Sheikhha ◽  
Seyed Mohammad Bagher Tabei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Therapy ◽  
Femoral Artery ◽  
Critical Limb Ischemia ◽  
Limb Ischemia

Abstract Introduction Critical limb ischemia (CLI) is the most advanced form of peripheral arterial disease (PAD) characterized by ischemic rest pain and non-healing ulcers. Currently, the standard therapy for CLI is the surgical reconstruction and endovascular therapy or limb amputation for patients with no treatment options. Neovasculogenesis induced by mesenchymal stem cells (MSCs) therapy is a promising approach to improve CLI. Owing to their angiogenic and immunomodulatory potential, MSCs are perfect candidates for the treatment of CLI. The purpose of this study was to determine and compare the in vitro and in vivo effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) on CLI treatment. Methods For the first step, BM-MSCs and AT-MSCs were isolated and characterized for the characteristic MSC phenotypes. Then, femoral artery ligation and total excision of the femoral artery were performed on C57BL/6 mice to create a CLI model. The cells were evaluated for their in vitro and in vivo biological characteristics for CLI cell therapy. In order to determine these characteristics, the following tests were performed: morphology, flow cytometry, differentiation to osteocyte and adipocyte, wound healing assay, and behavioral tests including Tarlov, Ischemia, Modified ischemia, Function and the grade of limb necrosis scores, donor cell survival assay, and histological analysis. Results Our cellular and functional tests indicated that during 28 days after cell transplantation, BM-MSCs had a great effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared with AT-MSCs and control groups. Conclusions Allogeneic BM-MSC transplantation resulted in a more effective recovery from critical limb ischemia compared to AT-MSCs transplantation. In fact, BM-MSC transplantation could be considered as a promising therapy for diseases with insufficient angiogenesis including hindlimb ischemia.

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