Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cells derived from human follicular fluid to oocyte‐like cell

2020 ◽  
Vol 45 (1) ◽  
pp. 127-139
Author(s):  
Mahin Taheri ◽  
Ghasem Saki ◽  
Roshan Nikbakht ◽  
Ali R. Eftekhari
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein ◽  
Follicular Fluid ◽  
Human Follicular Fluid ◽  
Morphogenetic Protein ◽  
Bone Morphogenetic Protein 15

scholarly journals Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell

2019 ◽  
Author(s):  
Mahin Taheri Moghadam ◽  
Ali Reza Eftekhari Moghadam ◽  
Ghasem Saki ◽  
Roshan Nikbakht
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Follicular Fluid ◽  
Germ Line ◽  
Stem Cell Markers ◽  
Human Follicular Fluid ◽  
Differentiation Potential ◽  
Morphogenetic Protein ◽  
Bone Morphogenetic Protein 15

Background. To study the effect of Bone morphogenetic protein 15 on differentiation potential of mesenchymal stem cell derived from human follicular fluid to oocyte like cell. Methods. Human FF derived cells were collected from 78 women in assisted fertilization program, and cultured in differentiation medium containing human recombinant BMP15 for 21 days. Mesenchymal stem cells and OLCs were characterized by real-time PCR and immunocytochemistry (ICC) staining. Results. MSCs expressed germ line stem cell markers, such as OCT4 and NANOG. After 15 days, OLCs formed and expressed zona pellucida markers (ZP2, ZP3), and reached 20 – 30 µm in diameters. Ten days after induction with BMP15, round cells remarkably developed, and the maximum size of OLCs reached 115 µm. Finally, a decrease ranging from 0.04 to 4.5 in the expression of pluripotency and oocyte specific markers was observed in the cells cultured in BMP15 supplemented medium. Our work demonstrates, FF derived MSCs have an innate potency to differentiate into OLCs, and BMP15 is effective in stimulating the differentiation of these cells, which may give an in vitro model to examine human germ cell development.

scholarly journals Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell

2019 ◽  
Author(s):  
Mahin Taheri Moghadam ◽  
Ali Reza Eftekhari Moghadam ◽  
Ghasem Saki ◽  
Roshan Nikbakht
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Follicular Fluid ◽  
Germ Line ◽  
Stem Cell Markers ◽  
Human Follicular Fluid ◽  
Differentiation Potential ◽  
Morphogenetic Protein ◽  
Bone Morphogenetic Protein 15

Background. To study the effect of Bone morphogenetic protein 15 on differentiation potential of mesenchymal stem cell derived from human follicular fluid to oocyte like cell. Methods. Human FF derived cells were collected from 78 women in assisted fertilization program, and cultured in differentiation medium containing human recombinant BMP15 for 21 days. Mesenchymal stem cells and OLCs were characterized by real-time PCR and immunocytochemistry (ICC) staining. Results. MSCs expressed germ line stem cell markers, such as OCT4 and NANOG. After 15 days, OLCs formed and expressed zona pellucida markers (ZP2, ZP3), and reached 20 – 30 µm in diameters. Ten days after induction with BMP15, round cells remarkably developed, and the maximum size of OLCs reached 115 µm. Finally, a decrease ranging from 0.04 to 4.5 in the expression of pluripotency and oocyte specific markers was observed in the cells cultured in BMP15 supplemented medium. Our work demonstrates, FF derived MSCs have an innate potency to differentiate into OLCs, and BMP15 is effective in stimulating the differentiation of these cells, which may give an in vitro model to examine human germ cell development.

scholarly journals Adenovirus-mediated bone morphogenetic protein-2 promotes osteogenic differentiation in human mesenchymal stem cells in vitro

2017 ◽  
Vol 14 (1) ◽  
pp. 377-382 ◽  
Author(s):  
Hong Cao ◽  
Zhi-Bo Sun ◽  
Lei Zhang ◽  
Wei Qian ◽  
Chun-Yang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Bone Morphogenetic Protein ◽  
Human Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

Bone morphogenetic protein-2 regulates proliferation of human mesenchymal stem cells

2003 ◽  
Vol 11 (5) ◽  
pp. 354-360 ◽  
Author(s):  
Kozo Akino ◽  
Takao Mineta ◽  
Masashi Fukui ◽  
Tohru Fujii ◽  
Sadanori Akita
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein ◽  
Human Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

scholarly journals CXCL12/CXCR4 Signal Axis Plays an Important Role in Mediating Bone Morphogenetic Protein 9-induced Osteogenic Differentiation of Mesenchymal Stem Cells

2013 ◽  
Vol 10 (9) ◽  
pp. 1181-1192 ◽  
Author(s):  
Chen Liu ◽  
Yaguang Weng ◽  
Taixian Yuan ◽  
Hong Zhang ◽  
Huili Bai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Bone Morphogenetic Protein ◽  
Morphogenetic Protein ◽  
Bone Morphogenetic Protein 9

The Additive Effect of Mesenchymal Stem Cells and Bone Morphogenetic Protein 2 on γ-Irradiated Bone Marrow in Mice

2011 ◽  
Vol 61 (3) ◽  
pp. 539-550 ◽  
Author(s):  
Shuibing Liu ◽  
Peizhen Hu ◽  
Ying Hou ◽  
Peng Li ◽  
Xubo Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein ◽  
Additive Effect ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein
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