scholarly journals Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell

2019 ◽  
Author(s):  
Mahin Taheri Moghadam ◽  
Ali Reza Eftekhari Moghadam ◽  
Ghasem Saki ◽  
Roshan Nikbakht
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Follicular Fluid ◽  
Germ Line ◽  
Stem Cell Markers ◽  
Human Follicular Fluid ◽  
Differentiation Potential ◽  
Morphogenetic Protein ◽  
Bone Morphogenetic Protein 15

Background. To study the effect of Bone morphogenetic protein 15 on differentiation potential of mesenchymal stem cell derived from human follicular fluid to oocyte like cell. Methods. Human FF derived cells were collected from 78 women in assisted fertilization program, and cultured in differentiation medium containing human recombinant BMP15 for 21 days. Mesenchymal stem cells and OLCs were characterized by real-time PCR and immunocytochemistry (ICC) staining. Results. MSCs expressed germ line stem cell markers, such as OCT4 and NANOG. After 15 days, OLCs formed and expressed zona pellucida markers (ZP2, ZP3), and reached 20 – 30 µm in diameters. Ten days after induction with BMP15, round cells remarkably developed, and the maximum size of OLCs reached 115 µm. Finally, a decrease ranging from 0.04 to 4.5 in the expression of pluripotency and oocyte specific markers was observed in the cells cultured in BMP15 supplemented medium. Our work demonstrates, FF derived MSCs have an innate potency to differentiate into OLCs, and BMP15 is effective in stimulating the differentiation of these cells, which may give an in vitro model to examine human germ cell development.

scholarly journals Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell

2019 ◽  
Author(s):  
Mahin Taheri Moghadam ◽  
Ali Reza Eftekhari Moghadam ◽  
Ghasem Saki ◽  
Roshan Nikbakht
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Follicular Fluid ◽  
Germ Line ◽  
Stem Cell Markers ◽  
Human Follicular Fluid ◽  
Differentiation Potential ◽  
Morphogenetic Protein ◽  
Bone Morphogenetic Protein 15

Background. To study the effect of Bone morphogenetic protein 15 on differentiation potential of mesenchymal stem cell derived from human follicular fluid to oocyte like cell. Methods. Human FF derived cells were collected from 78 women in assisted fertilization program, and cultured in differentiation medium containing human recombinant BMP15 for 21 days. Mesenchymal stem cells and OLCs were characterized by real-time PCR and immunocytochemistry (ICC) staining. Results. MSCs expressed germ line stem cell markers, such as OCT4 and NANOG. After 15 days, OLCs formed and expressed zona pellucida markers (ZP2, ZP3), and reached 20 – 30 µm in diameters. Ten days after induction with BMP15, round cells remarkably developed, and the maximum size of OLCs reached 115 µm. Finally, a decrease ranging from 0.04 to 4.5 in the expression of pluripotency and oocyte specific markers was observed in the cells cultured in BMP15 supplemented medium. Our work demonstrates, FF derived MSCs have an innate potency to differentiate into OLCs, and BMP15 is effective in stimulating the differentiation of these cells, which may give an in vitro model to examine human germ cell development.

Differentiation of human pluripotent teratocarcinoma stem cells induced by bone morphogenetic protein-2

1998 ◽  
Vol 10 (8) ◽  
pp. 551 ◽  
Author(s):  
Martin F. Pera ◽  
Daniella Herszfeld
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Retinoic Acid ◽  
Cell Line ◽  
Bone Morphogenetic Protein ◽  
Morphological Changes ◽  
Bone Morphogenetic Protein 2 ◽  
Stem Cell Markers ◽  
Potent Inducer ◽  
Morphogenetic Protein

Pluripotent human teratocarcinoma stem cells cultured in vitro provide a resource for the study of early embryonic development in man, as well as a means for discovery of novel factors controlling cell differentiation and commitment. We previously reported that the human teratocarcinoma stem cell line GCT 27X-1 could be induced to differentiate into an endodermal progenitor cell by treatment with high doses of retinoic acid. A search for polypeptide inducers of differentiation in this system has identified bone morphogenetic protein-2 (BMP-2) as a potent inducer of differentiation. In cell line GCT 27X-1, treatment with BMP-2 reduces proliferation, induces morphological changes similar to obtained following treatment with retinoic acid, and causes a decrease in the expression of transcripts for the stem cell markers CD30 and Oct-4. Preliminary immunochemical studies indicate that the differentiated cells produced by BMP-2 are endodermal precursors with a pattern of marker expression similar to that found in retinoic acid treated cells. Models of endoderm differentiation in humans will be useful for identifying the molecules which mediate cell interactions in development, and in achieving directed differentiation of cells for use in transplantation.

scholarly journals Mesenchymal Stem Cell-mediated Gene Delivery of Bone Morphogenetic Protein-2 in an Articular Fracture Model

2007 ◽  
Vol 15 (8) ◽  
pp. 1543-1550 ◽  
Author(s):  
Terri Zachos ◽  
Alisha Diggs ◽  
Steven Weisbrode ◽  
Jeffrey Bartlett ◽  
Alicia Bertone
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Mesenchymal Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Fracture Model ◽  
Bone Morphogenetic Protein 2 ◽  
Articular Fracture ◽  
Morphogenetic Protein
Sign in / Sign up

Export Citation Format

Share Document