Amino acids in compound feed when rearing replacement young quails

In the technology of production of quail eggs it is important to prepare the replacement young quails for egg laying since the timing of the beginning and duration of the productive period, the level of egg production, and the quality of eggs depend on it. A prerequisite for obtaining high productivity of laying quails in the future is a complete feeding during the rearing period. One solution to this problem is the use of synthetic amino acids in poultry feeding, which increase the biological value of the feed protein to meet the poultry’s protein needs. The results of research on the use of compound feed with an increased content of amino acids (lysine and methionine with cystine) by 15 and 20 % in the nutritional content of replacement young quails of Omskaya breed of meat and egg productivity have been presented in the article. It has been found that the use of experimental compound feed contributed to the increase in the live weight of quails of the experimental groups by 3,94 and 2,97 %, the decrease in the average daily feed consumption by 11,21 and 7,56 %, and expenditures of compound feed for 1 kg of gain by 14,54 and 8,31 %. The higher content of amino acids has increased the yield of effective young quails in the experimental groups by 4,09 and 7,07 %. It has been found in the result of carrying out anatomical cutting of carcasses that feeding quails with experimental compound feed had a stimulating effect on the development of egg-forming organs. The calculation of the economic efficiency of rearing replacement young quails has shown that the use of compound feed with the increase in amino acids by 15 and 20 % in compound feed reduced the expenditures of their rearing by 8,04 and 5,28 % and the prime-cost of one replacement female quail by 12,86 and 11,63 %.

RESULTS OF ORGANOLEPTIC AND TASTING EVALUATION OF POULTRY MEAT WHEN INTRODUCING THE REGULATORY COMPLEX "BYPASS" INTO THE DIET

Stimulation of the body of poultry by the regulatory complex "Bypass" allowed us to obtain a high-quality product. The removal of synthetic amino acids from the feed helps to improve the taste of the meat of broiler chickens of the «Ross-308» cross.

1 Introduction: Review of the Career: Gary L. Allee

Gary Allee’s professional career as a swine nutritionist spans from 1972 to 2020 in the peer-reviewed publication record. An evaluation of the major themes presented in his publications show a connectivity of work done at a “basic” science level leading to work on the application of this basic knowledge to meet pig production needs. It is interesting to explore the themes of Dr. Allee’s work and connect that to how pigs are fed on a practical basis today. Regarding sow nutrition work, Dr. Allee used his early work on lipogenesis to expand on the topic to understand how energy sources are able to cross the placental barrier and ultimately impact neonatal energy reserves. Further, Dr. Allee responded to availability changes in synthetic amino acids to the industry to explore opportunities to leverage these AA’s into sow diet formulations. In the area of nursery pig nutrition, his record explores the theme of lipid metabolism from lipogenesis, to ketogenesis to fat source and fatty acid profile implication to diet value. However, Dr. Allee picked up the themes of protein and lactose sources and implications on hypersensitivity and value of proteins and sugars targeted to the young pig all influencing practical diet formulations globally in young pig nutrition. In the area of the grow-finish pig, Gary’s work explored topics of dietary fat use, practical aspects of amino acid inclusion and the interaction of repartitioning approaches to lean deposition with nutrient requirements. This symposium is designed to highlight several key principals for success as a scientist that are evident in Dr. Allee’s career: a) importance of being a systems thinker in ability to take basic information and evolve it into practical implications; b) importance of being a global citizen in scientific professional development; and c) the impact that one has on people endures.

PSVI-17 The Effect of Dacitic Tuff Breccia Alone or in Combination with Poultry By-products on Nursery Pig Growth Performance

Dacitic tuff breccia (DTB) and poultry by-products (PBP) were added to nursery pig diets to evaluate their effects on growth performance in a 35 d experiment. A randomized complete block design based on BW and sex was used. Newly weaned pigs (N=564, 20.1 d of age, 6.18±0.006 kg initial BW) allotted to four diets (15 replicates/treatment; 9 or 10 pigs/pen): 1) Negative control (NC; no DTB or PBP), 2) NC+DTB (0.5% inclusion), 3) NC+PBP (4% chicken by-product meal + 2% feather meal – replacing fishmeal, corn, and synthetic amino acids in the NC), 4) NC+DTB+PBP. Phase 1–3 were each 7-d and Phase 4 was from d21-35. During week one there were no differences (P >0.10) among treatments. In week 2, pigs fed DTB tended to increase ADG (323 vs. 303 g/d; P=0.068) and ADFI (376 vs. 356 g/d; P=0.055) compared to pigs not fed DTB and pigs fed PBP tended to have increased ADG (P=0.093). In week 3 pigs fed PBP had reduced ADG (460 vs 483 g/d; P=0.011) and G:F (0.689 vs. 0.723; P< 0.001), and there tended to be an interaction for G:F (P=0.083) with DTB improving G:F when fed in combination with PBP but reducing G:F in the control diet. In week 4, feeding PBP decreased ADG (384 vs 415 g/d; P< 0.01) and ADFI (629 vs 666 g/d; P< 0.01) compared to pigs fed no PBP. There were no differences among treatments during week 5. For Phase 4 (d21-35) pigs fed PBP had decreased ADG (479 vs 497 g/d; P=0.041) and tended to have reduced ADFI (P=0.092) compared to pigs fed no PBP. Overall (d 0–35), there were no significant differences among treatments. In conclusion, DTB improved nursery pig growth performance early, however, pigs fed PBP had decreased ADG and feed efficiency late in the nursery period.

GC-MS Discrimination of Citrulline from Ornithine and Homocitrulline from Lysine by Chemical Derivatization: Evidence of Formation of N5-Carboxy-ornithine and N6-Carboxy-lysine

Derivatization of amino acids by 2 M HCl/CH3OH (60 min, 80 °C) followed by derivatization of the intermediate methyl esters with pentafluoropropionic anhydride (PFPA) in ethyl acetate (30 min, 65 °C) is a useful two-step derivatization procedure (procedure A) for their quantitative measurement in biological samples by gas chromatography-mass spectrometry (GC-MS) as methyl ester pentafluoropropionic (PFP) derivatives, (Me)m-(PFP)n. This procedure allows in situ preparation of trideutero-methyl esters PFP derivatives, (d3Me)m-(PFP)n, from synthetic amino acids and 2 M HCl/CD3OD for use as internal standards. However, procedure A converts citrulline (Cit) to ornithine (Orn) and homocitrulline (hCit) to lysine (Lys) due to the instability of their carbamide groups under the acidic conditions of the esterification step. In the present study, we investigated whether reversing the order of the two-step derivatization may allow discrimination and simultaneous analysis of these amino acids. Pentafluoropropionylation (30 min, 65 °C) and subsequent methyl esterification (30 min, 80 °C), i.e., procedure B, of Cit resulted in the formation of six open and cyclic reaction products. The most abundant product is likely to be N5-Carboxy-Orn. The second most abundant product was confirmed to be Orn. The most abundant reaction product of hCit was confirmed to be Lys, with the minor reaction product likely being N6-Carboxy-Lys. Mechanisms are proposed for the formation of the reaction products of Cit and hCit via procedure B. It is assumed that at the first derivatization step, amino acids form (N,O)-PFP derivatives including mixed anhydrides. At the second derivatization step, the Cit-(PFP)4 and hCit-(PFP)4 are esterified on their C1-Carboxylic groups and on their activated Nureido groups. Procedure B also allows in situ preparation of (d3Me)m-(PFP)n from synthetic amino acids for use as internal standards. It is demonstrated that the derivatization procedure B enables discrimination between Cit and Orn, and between hCit and Lys. The utility of procedure B to measure simultaneously these amino acids in biological samples such as plasma and urine remains to be demonstrated. Further work is required to optimize the derivatization conditions of procedure B for biological amino acids.

Sustainable Electrochemical Decarboxylative Acetoxylation of Aminoacids in Batch and Continuous Flow

Introduction of acetoxy groups to organic molecules is important for the preparation of many active ingredients and synthetic intermediates. A commonly used and attractive strategy is the oxidative decarboxylation of aliphatic carboxylic acids, which entails the generation of a new C(sp3)-O bond. This reaction has been traditionally carried out using excess amounts of harmful lead(IV) acetate. A sustainable alternative to stoichiometric oxidants is the Hofer-Moest reaction, which relies in the 2-electron anodic oxidation of the carboxylic acid. However, examples showing electrochemical acetoxylation of amino acids are scarce. Herein we present a general and scalable procedure for the anodic decarboxylative acetoxylation of amino acids in batch and continuous flow mode. The procedure has been applied to the derivatization of several natural and synthetic amino acids, including key intermediates for the synthesis of active pharmaceutical ingredients. Good to excellent yields were obtained in all cases. Transfer of the process from batch to a continuous flow cell signficantly increased reaction throughput and space-time yield, with excellent product yields obtained even in a single-pass. The sustainability of the electrochemical protocol has been examined by evaluating its green metrics. Comparison with the conventional method demonstrates that an electrochemical approach has a significant positive effect on the greenness of the process

Sustainable Electrochemical Decarboxylative Acetoxylation of Aminoacids in Batch and Continuous Flow

Introduction of acetoxy groups to organic molecules is important for the preparation of many active ingredients and synthetic intermediates. A commonly used and attractive strategy is the oxidative decarboxylation of aliphatic carboxylic acids, which entails the generation of a new C(sp3)-O bond. This reaction has been traditionally carried out using excess amounts of harmful lead(IV) acetate. A sustainable alternative to stoichiometric oxidants is the Hofer-Moest reaction, which relies in the 2-electron anodic oxidation of the carboxylic acid. However, examples showing electrochemical acetoxylation of amino acids are scarce. Herein we present a general and scalable procedure for the anodic decarboxylative acetoxylation of amino acids in batch and continuous flow mode. The procedure has been applied to the derivatization of several natural and synthetic amino acids, including key intermediates for the synthesis of active pharmaceutical ingredients. Good to excellent yields were obtained in all cases. Transfer of the process from batch to a continuous flow cell signficantly increased reaction throughput and space-time yield, with excellent product yields obtained even in a single-pass. The sustainability of the electrochemical protocol has been examined by evaluating its green metrics. Comparison with the conventional method demonstrates that an electrochemical approach has a significant positive effect on the greenness of the process

PERFORMANCE AND CARCASS EVALUATION OF BROILER CHICKS FED SHRIMP WASTE MEAL SUPPLEMENTED WITH SYNTHETIC AMINO ACIDS

A feeding trial was conducted using 104 one week-old anak broilier chicks to evaluate the replacement value of shrimp waste meal (SWM) for fish meal (FM) in broiler diets. Fish meal contribution to the dietary crude protein of the brioiler dietsat was replaced with SWM 0, 33, 66 and 100% of graded levels. Weight gain and feedconverstion ratio were significantly (P > 0.05) reduced with SWM replacing FM at 100% at both the starter and finisher phases. No significant differences (P >0.05) were observed for feed consumption. mortality increased with an increased levle of SWM in the diet at the starter phase. Liver weight increased (P <0.05) with increased level of SWM significantly (P <0.05) increased the abdominal fat. Result of this present study show that the SWM could partially replace 66% of the crude protein of FM in broiler diets without negatively affecting performance or carcass quality

ADIPOSE TISSUE AS THE MOST IMPORTANT ELEMENT DETERMINING THE QUALITY OF PORK

The article presents the materials of research conducted on fattening growing young pigs of large white breed, in the diet of which synthetic amino acids were used. As a result of experiments, the high efficiency of their use was confirmed. The positive effect of the use of synthetic amino acids on the formation of more optimal indicators of the chemical composition of fat is proved. Experiments have shown that the most effective productive effect of feed was shown in group IV animals that received a basic diet balanced in amino acids due to the introduction of synthetic lysine and feed methionine.

Fine-tuning of lysine side chain modulates the activity of histone lysine methyltransferases

Histone lysine methyltransferases (KMTs) play an important role in epigenetic gene regulation and have emerged as promising targets for drug discovery. However, the scope and limitation of KMT catalysis on substrates possessing substituted lysine side chains remain insufficiently explored. Here, we identify new unnatural lysine analogues as substrates for human methyltransferases SETD7, SETD8, G9a and GLP. Two synthetic amino acids that possess a subtle modification on the lysine side chain, namely oxygen at the γ position (KO, oxalysine) and nitrogen at the γ position (KN, azalysine) were incorporated into histone peptides and tested as KMTs substrates. Our results demonstrate that these lysine analogues are mono-, di-, and trimethylated to a different extent by trimethyltransferases G9a and GLP. In contrast to monomethyltransferase SETD7, SETD8 exhibits high specificity for both lysine analogues. These findings are important to understand the substrate scope of KMTs and to develop new chemical probes for biomedical applications.