ChemInform Abstract: A Practical Approach to the Implementation of Selectivity in Homonuclear Multidimensional NMR with Frequency Selective-Filtering Techniques. Application to the Chemical Structure Elucidation of Complex Oligosaccharides

We performed a high-content screening (HCS) assay aiming to discover bioactive molecules with proteasome inhibitory activity. By structural elucidation, we identified six compounds purified from soft coral Clavularia flava, which potentiates proteasome inhibition. Chemical structure elucidation revealed they are dolabellane- and secosteroid-based compounds including a new dolabellane, clavinflol C (1), three known dolabellanes, stolonidiol (2), stolonidiol-17-acetate (3), and clavinflol B (4) as well as two new secosteroids, 3β,11-dihydroxy-24-methyl-9,11-secocholest-5-en-9,23-dione (5) and 3β,11-dihydroxy-24-methylene-9,11-secocholest-5-en-9,23-dione (6). All six compounds show less cytotoxicity than those of known proteasome inhibitors, bortezomib and MG132. In summary, the high-content measurements of control inhibitors, bortezomib and MG132, manifest the highest ratio >2 in high-content measurement. Of the isolated compounds, 2 and 5 showed higher activity, followed by 3 and 6, and then 1 and 4 exhibited moderate inhibition.

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Sequential Interconnected Interpenetrating Polymer Networks of Polyurethane and Polystyrene. 1. Synthesis and Chemical Structure Elucidation

Macromolecules ◽

10.1021/ma00094a024 ◽

1994 ◽

Vol 27 (16) ◽

pp. 4583-4594 ◽

Cited By ~ 14

Author(s):

S. B. Pandit ◽

V. M. Nadkarni

Keyword(s):

Structure Elucidation ◽

Chemical Structure ◽

Polymer Networks ◽

Interpenetrating Polymer Networks

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Chemical structure elucidation of total lignins in woods. Part II: Analysis of a fraction of residual wood left after MWL isolation and solubilized in lithium chloride/N,N-dimethylacetamide

Holzforschung ◽

10.1515/hf.2006.110 ◽

2006 ◽

Vol 60 (6) ◽

pp. 653-658 ◽

Cited By ~ 9

Author(s):

Hiroyuki Furuno ◽

Toshiyuki Takano ◽

Shuichi Hirosawa ◽

Hiroshi Kamitakahara ◽

Fumiaki Nakatsubo

Keyword(s):

Lithium Chloride ◽

Structure Elucidation ◽

Chemical Structure ◽

Soluble Fraction ◽

Elution Curve ◽

Mass Region ◽

Milled Wood Lignin ◽

Cellulose Solvent ◽

Lignin Fraction ◽

Partial Degradation

Abstract The residual wood meal left after extraction of milled wood lignin (MWL) was extracted with lithium chloride/N,N-dimethylacetamide, which is a well-known cellulose solvent, to afford a soluble fraction (cellulose-lignin fraction; CL) in 36.7% yield. The UV elution curve of CL acetate has the same profile as its refractive index (RI) elution curve. After partial degradation of CL by cellulase, the UV elution curve of CL acetate shifted to the low-molecular-mass region in a similar fashion as its RI elution curve. These results indicate that the lignin in CL (CL lignin) is chemically bonded to cellulose. On the other hand, half of the CL lignin was removed by xylanase treatment. It was concluded that approximately half of the CL lignin existed as a lignin-cellulose-xylan complex.

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