ChemInform Abstract: Biomarkers for Drug Discovery: Important Aspects of in vitro Assay Design for HTS and HCS Bioassays

ChemInform ◽  
2008 ◽  
Vol 39 (33) ◽  
Author(s):  
William A. Irwin ◽  
Dubravko Jelic ◽  
Roberto Antolovic
Keyword(s):  
Drug Discovery ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Assay Design

scholarly journals Non-invasive monitoring of drug action: A new live in vitro assay design for Chagas’ disease drug discovery

2020 ◽  
Vol 14 (7) ◽  
pp. e0008487
Author(s):  
Anna F. Fesser ◽  
Olivier Braissant ◽  
Francisco Olmo ◽  
John M. Kelly ◽  
Pascal Mäser ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Chagas Disease ◽  
Drug Action ◽  
In Vitro Assay ◽  
Invasive Monitoring ◽  
Vitro Assay ◽  
Non Invasive ◽  
Assay Design

AN IN VITRO ASSAY OF CORTICOTROPHIN

1955 ◽  
Vol 33 (2) ◽  
pp. 209-216 ◽  
Author(s):  
K. W. McKerns ◽  
E. Nordstrand
Keyword(s):  
Dose Response ◽  
In Vitro Assay ◽  
Assay Method ◽  
Response Curves ◽  
Vitro Assay ◽  
Rat Adrenals ◽  
Assay Design ◽  
Dose Response Curves ◽  
Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

AN IN VITRO ASSAY OF CORTICOTROPHIN

1955 ◽  
Vol 33 (1) ◽  
pp. 209-216
Author(s):  
K. W. McKerns ◽  
E. Nordstrand
Keyword(s):  
Dose Response ◽  
In Vitro Assay ◽  
Assay Method ◽  
Response Curves ◽  
Vitro Assay ◽  
Rat Adrenals ◽  
Assay Design ◽  
Dose Response Curves ◽  
Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

Predicting Phospholipidosis: A Fluorescence Noncell Based in Vitro Assay for the Determination of Drug–Phospholipid Complex Formation in Early Drug Discovery

2011 ◽  
Vol 83 (18) ◽  
pp. 6980-6987 ◽  
Author(s):  
Liping Zhou ◽  
Gina Geraci ◽  
Sloan Hess ◽  
Linhong Yang ◽  
Jianling Wang ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Complex Formation ◽  
In Vitro Assay ◽  
Phospholipid Complex ◽  
Vitro Assay ◽  
Early Drug

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

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