ChemInform Abstract: 2H-Pyran-2-one and 2H-Furan-2-one Derivatives from the Plant Endophytic Fungus Pestalotiopsis fici.

ChemInform ◽  
2014 ◽  
Vol 45 (13) ◽  
pp. no-no
Author(s):  
Shuchun Liu ◽  
Xiangyu Liu ◽  
Liangdong Guo ◽  
Yongsheng Che ◽  
Ling Liu
2017 ◽  
Vol 101 (4) ◽  
pp. 1593-1604 ◽  
Author(s):  
Shu Zhang ◽  
Xiu-Na Wang ◽  
Xiao-Ling Zhang ◽  
Xing-Zhong Liu ◽  
Yong-Jie Zhang

Fitoterapia ◽  
2013 ◽  
Vol 85 ◽  
pp. 114-118 ◽  
Author(s):  
Shuchun Liu ◽  
Liangdong Guo ◽  
Yongsheng Che ◽  
Ling Liu

RSC Advances ◽  
2015 ◽  
Vol 5 (96) ◽  
pp. 78708-78711 ◽  
Author(s):  
Ling Liu ◽  
Chen Zhao ◽  
Li Li ◽  
Liangdong Guo ◽  
Yongsheng Che

The endophytic fungus Pestalotiopsis fici produced pestalotriols A (1) and B (2) possessing the unique spiro[2.5]octane skeleton.


ChemInform ◽  
2016 ◽  
Vol 47 (6) ◽  
pp. no-no
Author(s):  
Ling Liu ◽  
Chen Zhao ◽  
Li Li ◽  
Liangdong Guo ◽  
Yongsheng Che

ChemInform ◽  
2016 ◽  
Vol 47 (28) ◽  
Author(s):  
Bo Wang ◽  
Zhuowei Zhang ◽  
Liangdong Guo ◽  
Ling Liu

2021 ◽  
Vol 7 (10) ◽  
pp. 809
Author(s):  
Xinran Xu ◽  
Runye Huang ◽  
Wen-Bing Yin

Endophytic fungi are emerging as attractive producers of natural products with diverse bioactivities and novel structures. However, difficulties in the genetic manipulation of endophytic fungi limit the search of novel secondary metabolites. In this study, we improved the polyethylene glycol (PEG)-mediated protoplast transformation method by introducing the CRISPR/Cas9 system into endophytic fungus Pestalotiopsis fici. Using this approach, we performed genome editing such as site-specific gene insertion, dual-locus mutations, and long DNA fragment deletions in P. fici efficiently. The average efficiency for site-specific gene insertion and two-site gene editing was up to 48.0% and 44.4%, respectively. In addition, the genetic manipulation time with long DNA fragment (5–10 kb) deletion was greatly shortened to one week in comparison with traditional methods such as Agrobacterium tumefaciens-mediated transformation (ATMT). Taken together, the development of the CRISPR/Cas9 system in the endophytic fungus will accelerate the discovery of novel natural products and further biological study.


2009 ◽  
Vol 17 (2) ◽  
pp. 606-613 ◽  
Author(s):  
Ling Liu ◽  
Shuchun Liu ◽  
Xulin Chen ◽  
Liangdong Guo ◽  
Yongsheng Che

2008 ◽  
Vol 16 (11) ◽  
pp. 6021-6026 ◽  
Author(s):  
Ling Liu ◽  
Renrong Tian ◽  
Shuchun Liu ◽  
Xulin Chen ◽  
Liangdong Guo ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document