scholarly journals Diagnostic concordance between whole slide imaging and conventional light microscopy in cytopathology: A systematic review

2019 ◽  
Vol 128 (1) ◽  
pp. 17-28 ◽  
Author(s):  
Ilaria Girolami ◽  
Liron Pantanowitz ◽  
Stefano Marletta ◽  
Matteo Brunelli ◽  
Claudia Mescoli ◽  
...  
Keyword(s):  
Systematic Review ◽  
Light Microscopy ◽  
Whole Slide Imaging ◽  
Diagnostic Concordance ◽  
Conventional Light Microscopy

scholarly journals The Diagnostic Concordance of Whole Slide Imaging and Light Microscopy: A Systematic Review

2016 ◽  
Vol 141 (1) ◽  
pp. 151-161 ◽  
Author(s):  
Edward Goacher ◽  
Rebecca Randell ◽  
Bethany Williams ◽  
Darren Treanor
Keyword(s):  
Systematic Review ◽  
Light Microscopy ◽  
Primary Diagnosis ◽  
Cochrane Library ◽  
Whole Slide Imaging ◽  
Tissue Samples ◽  
The Mean ◽  
Diagnostic Concordance ◽  
High Level ◽  
The Cochrane Library

Context.—Light microscopy (LM) is considered the reference standard for diagnosis in pathology. Whole slide imaging (WSI) generates digital images of cellular and tissue samples and offers multiple advantages compared with LM. Currently, WSI is not widely used for primary diagnosis. The lack of evidence regarding concordance between diagnoses rendered by WSI and LM is a significant barrier to both regulatory approval and uptake. Objective.—To examine the published literature on the concordance of pathologic diagnoses rendered by WSI compared with those rendered by LM. Data Sources.—We conducted a systematic review of studies assessing the concordance of pathologic diagnoses rendered by WSI and LM. Studies were identified following a systematic search of Medline (Medline Industries, Mundelein, Illinois), Medline in progress (Medline Industries), EMBASE (Elsevier, Amsterdam, the Netherlands), and the Cochrane Library (Wiley, London, England), between 1999 and March 2015. Conclusions.—Thirty-eight studies were included in the review. The mean diagnostic concordance of WSI and LM, weighted by the number of cases per study, was 92.4%. The weighted mean κ coefficient between WSI and LM was 0.75, signifying substantial agreement. Of the 30 studies quoting percentage concordance, 18 (60%) showed a concordance of 90% or greater, of which 10 (33%) showed a concordance of 95% or greater. This review found evidence to support a high level of diagnostic concordance. However, there were few studies, many were small, and they varied in quality, suggesting that further validation studies are still needed.

Whole slide imaging diagnostic concordance with light microscopy for breast needle biopsies

2014 ◽  
Vol 45 (8) ◽  
pp. 1713-1721 ◽  
Author(s):  
W. Scott Campbell ◽  
Steven H. Hinrichs ◽  
Subodh M. Lele ◽  
John J. Baker ◽  
Audrey J. Lazenby ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Whole Slide Imaging ◽  
Diagnostic Concordance

scholarly journals Validating Whole Slide Imaging Systems for Diagnostic Purposes in Pathology: Guideline Update From the College of American Pathologists in Collaboration With the American Society for Clinical Pathology and the Association for Pathology Informatics

2021 ◽  
Author(s):  
Andrew J. Evans ◽  
Richard W. Brown ◽  
Marilyn M. Bui ◽  
Elizabeth A. Chlipala ◽  
Christina Lacchetti ◽  
...  
Keyword(s):  
Systematic Review ◽  
Expert Panel ◽  
Good Practice ◽  
Digital Pathology ◽  
Clinical Pathology ◽  
Glass Slide ◽  
Whole Slide Imaging ◽  
Assessment Development ◽  
Evaluation Approach ◽  
Diagnostic Concordance

Context.— The original guideline, “Validating Whole Slide Imaging for Diagnostic Purposes in Pathology,” was published in 2013 and included 12 guideline statements. The College of American Pathologists convened an expert panel to update the guideline following standards established by the National Academies of Medicine for developing trustworthy clinical practice guidelines. Objective.— To assess evidence published since the release of the original guideline and provide updated recommendations for validating whole slide imaging (WSI) systems used for diagnostic purposes. Design.— An expert panel performed a systematic review of the literature. Frozen sections, anatomic pathology specimens (biopsies, curettings, and resections), and hematopathology cases were included. Cytology cases were excluded. Using the Grading of Recommendations Assessment, Development, and Evaluation approach, the panel reassessed and updated the original guideline recommendations. Results.— Three strong recommendations and 9 good practice statements are offered to assist laboratories with validating WSI digital pathology systems. Conclusions.— Systematic review of literature following release of the 2013 guideline reaffirms the use of a validation set of at least 60 cases, establishing intraobserver diagnostic concordance between WSI and glass slides and the use of a 2-week washout period between modalities. Although all discordances between WSI and glass slide diagnoses discovered during validation need to be reconciled, laboratories should be particularly concerned if their overall WSI–glass slide concordance is less than 95%.

scholarly journals A Comparative Study Between Smartphone-Based Microscopy and Conventional Light Microscopy in 1021 Dermatopathology Specimens

2016 ◽  
Vol 140 (1) ◽  
pp. 86-90 ◽  
Author(s):  
Richard R. Jahan-Tigh ◽  
Garrett M. Chinn ◽  
Ronald P. Rapini
Keyword(s):  
Light Microscopy ◽  
Cell Carcinoma ◽  
Sensitivity And Specificity ◽  
Performance Characteristics ◽  
University Hospital ◽  
Inflammatory Conditions ◽  
Skin Cancers ◽  
And Performance ◽  
Nonmelanoma Skin Cancers ◽  
Conventional Light Microscopy

Context The incorporation of high-resolution cameras into smartphones has allowed for a variety of medical applications including the use of lens attachments that provide telescopic, macroscopic, and dermatoscopic data, but the feasibility and performance characteristics of such a platform for use in dermatopathology have not been described. Objective To determine the diagnostic performance of a smartphone microscope compared to traditional light microscopy in dermatopathology specimens. Design A simple smartphone microscope constructed with a 3-mm ball lens was used to prospectively evaluate 1021 consecutive dermatopathology cases in a blinded fashion. Referred, consecutive specimens from the community were evaluated at a single university hospital. The performance characteristics of the smartphone platform were calculated by using conventional light microscopy as the gold standard. The sensitivity and specificity for the diagnosis of melanoma, nonmelanoma skin cancers, and other miscellaneous conditions by the phone microscopy platform, as compared with traditional light microscopy, were calculated. Results For basal cell carcinoma (n = 136), the sensitivity and specificity of smartphone microscopy were 95.6% and 98.1%, respectively. The sensitivity and specificity for squamous cell carcinoma (n = 94) were 89.4% and 97.3%, respectively. The lowest sensitivity was found in melanoma (n = 15) at 60%, although the specificity was high at 99.1%. The accuracy of diagnosis of inflammatory conditions and other neoplasms was variable. Conclusions Mobile phone–based microscopy has excellent performance characteristics for the inexpensive diagnosis of nonmelanoma skin cancers in a setting where a traditional microscope is not available.

scholarly journals Cyanoprokaryotes biodiversity in nine shallow Bulgarian wetlands

2019 ◽  
Vol 2 ◽  
Author(s):  
Blagoy Uzunov ◽  
Maya Stoyneva-Gärtner ◽  
Georg Gärtner ◽  
Mariana Radkova ◽  
Katerina Stefanova
Keyword(s):  
Light Microscopy ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Molecular Methods ◽  
Geographical Pattern ◽  
Algal Toxins ◽  
The Real ◽  
Total Phytoplankton ◽  
Toxigenic Strains ◽  
Conventional Light Microscopy

The results from the investigation of the phytoplankton in nine shallow wetlands, situated in Central and Eastern Bulgaria, conducted in the frame of recent projects related to identifying harmful algal blooms and algal toxins in the country are demonstrated. Therefore, the focus is on the cyanoprokaryotes and their toxigenic species in particular. The species were identified using conventional light microscopy, while for distinguishing the toxigenic species and strains PCR-based molecular methods were applied. Cyanoprokaryotes comprised about 30% of the total phytoplankton biodiversity of the studied wetlands and were unevenly spread among them. The presence and spread of toxigenic strains in the studied wetlands also did not show any strong geographical pattern. Taxonomic problems related with the evaluation of the real cyanoprokaryotic biodiversity and potential alien or invasive character of the morphospecies are discussed.

Confocal Microscopy in Comparison with Electron and Conventional Light Microscopy

1987 ◽  
pp. 59-81 ◽  
Author(s):  
H.T.M. VAN DER VOORT ◽  
J.A.C. VALKENBURG ◽  
E.A. VAN SPRONSEN ◽  
C.L. WOLDRINGH ◽  
G.J. BRAKENHOFF
Keyword(s):  
Confocal Microscopy ◽  
Light Microscopy ◽  
Conventional Light Microscopy
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