Estrogen receptor-?, but not estrogen receptor-?, is expressed in prolactin neurons of the female rat paraventricular and supraoptic nuclei: Comparison with other neuropeptides

2005 ◽  
Vol 484 (1) ◽  
pp. 28-42 ◽  
Author(s):  
Shotaro Suzuki ◽  
Robert J. Handa
2003 ◽  
Vol 284 (1) ◽  
pp. E184-E192 ◽  
Author(s):  
Chris Stirone ◽  
Sue P. Duckles ◽  
Diana N. Krause

The cerebral vasculature is an important target tissue for estrogen, as evidenced by significant effects of estrogen on vascular reactivity and protein levels of endothelial nitric oxide synthase and prostacyclin synthase. However, the presence, localization, and regulation of estrogen receptors in the cerebral vasculature have not been investigated. In this study, we identified the presence of estrogen receptor-α (ER-α) in female rat cerebral blood vessels and localized this receptor to both smooth muscle and endothelial cells by use of immunohistochemistry and confocal microscopy. With immunoblot analysis, multiple forms of ER-α were detected at 110, 93, 82, 50, and 45 kDa in addition to a relatively weak band corresponding to the 66-kDa putative unmodified receptor. The 82-kDa band was identified as Ser118-phosphorylated ER-α, whereas the 50-kDa band lacks the normal NH2 terminus, suggestive of an ER-α splice variant. Lower molecular mass bands persisted after in vivo inhibition of 26S proteasome activity with lactacystin, whereas the 110- and 93-kDa bands increased. All forms of ER-α in cerebral vessels were decreased after ovariectomy but significantly increased after chronic estrogen exposure in vivo.


2016 ◽  
Vol 124 (5) ◽  
pp. 601-610 ◽  
Author(s):  
Janina Helle ◽  
Manuela I. Bader ◽  
Annekathrin M. Keiler ◽  
Oliver Zierau ◽  
Günter Vollmer ◽  
...  

1994 ◽  
Vol 6 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Robert E. Watson ◽  
Robert K. Hutchinson ◽  
M. Chris Langub ◽  
John W. Landis ◽  
Shikha Seksaria ◽  
...  

2010 ◽  
Vol 122 (5) ◽  
pp. 310-317 ◽  
Author(s):  
Laura S. Lubbers ◽  
Peter T. Zafian ◽  
Claris Gautreaux ◽  
Marisa Gordon ◽  
Stephen E. Alves ◽  
...  

2011 ◽  
Vol 31 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Nanette J. Tomicek ◽  
Jennifer L. Miller-Lee ◽  
J. Craig Hunter ◽  
Donna H. Korzick

2000 ◽  
Vol 875 (1-2) ◽  
pp. 56-65 ◽  
Author(s):  
Eun-Joo Lee ◽  
Constance T Moore ◽  
Somaya Hosny ◽  
Adrian Centers ◽  
Lothar Jennes

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